Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1908 and EX28DRAFT_1909 are separated by 81 nucleotides EX28DRAFT_1909 and EX28DRAFT_1910 are separated by 116 nucleotides
EX28DRAFT_1908: EX28DRAFT_1908 - Nucleoside permease, at 1,916,442 to 1,917,626
_1908
EX28DRAFT_1909: EX28DRAFT_1909 - Protein of unknown function (DUF3574), at 1,917,708 to 1,918,127
_1909
EX28DRAFT_1910: EX28DRAFT_1910 - sodium/proline symporter, at 1,918,244 to 1,919,752
_1910
Position (kb)
1917
1918
1919 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1916.902 kb on - strand, within EX28DRAFT_1908 at 1916.995 kb on - strand, within EX28DRAFT_1908 at 1916.995 kb on - strand, within EX28DRAFT_1908 at 1916.996 kb on + strand, within EX28DRAFT_1908 at 1916.996 kb on + strand, within EX28DRAFT_1908 at 1916.997 kb on - strand, within EX28DRAFT_1908 at 1917.062 kb on + strand, within EX28DRAFT_1908 at 1917.062 kb on + strand, within EX28DRAFT_1908 at 1917.163 kb on - strand, within EX28DRAFT_1908 at 1917.165 kb on - strand, within EX28DRAFT_1908 at 1917.259 kb on + strand, within EX28DRAFT_1908 at 1917.260 kb on - strand, within EX28DRAFT_1908 at 1917.292 kb on - strand, within EX28DRAFT_1908 at 1917.292 kb on - strand, within EX28DRAFT_1908 at 1917.295 kb on + strand, within EX28DRAFT_1908 at 1917.296 kb on - strand, within EX28DRAFT_1908 at 1917.296 kb on - strand, within EX28DRAFT_1908 at 1917.296 kb on - strand, within EX28DRAFT_1908 at 1917.296 kb on - strand, within EX28DRAFT_1908 at 1917.356 kb on + strand, within EX28DRAFT_1908 at 1917.356 kb on + strand, within EX28DRAFT_1908 at 1917.357 kb on - strand, within EX28DRAFT_1908 at 1917.357 kb on - strand, within EX28DRAFT_1908 at 1917.676 kb on + strand at 1917.676 kb on + strand at 1917.677 kb on - strand at 1917.677 kb on - strand at 1917.900 kb on + strand, within EX28DRAFT_1909 at 1917.994 kb on - strand, within EX28DRAFT_1909 at 1918.092 kb on + strand at 1918.147 kb on + strand at 1918.147 kb on + strand at 1918.148 kb on - strand at 1918.148 kb on - strand at 1918.149 kb on + strand at 1918.150 kb on - strand at 1918.150 kb on - strand at 1918.241 kb on + strand at 1918.245 kb on + strand at 1918.246 kb on - strand at 1918.282 kb on - strand at 1918.622 kb on - strand, within EX28DRAFT_1910 at 1918.622 kb on - strand, within EX28DRAFT_1910 at 1918.681 kb on - strand, within EX28DRAFT_1910 at 1918.931 kb on - strand, within EX28DRAFT_1910 at 1919.011 kb on - strand, within EX28DRAFT_1910
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 1,916,902 - EX28DRAFT_1908 0.39 +0.3 1,916,995 - EX28DRAFT_1908 0.47 -1.2 1,916,995 - EX28DRAFT_1908 0.47 -0.6 1,916,996 + EX28DRAFT_1908 0.47 -0.6 1,916,996 + EX28DRAFT_1908 0.47 -0.9 1,916,997 - EX28DRAFT_1908 0.47 -0.7 1,917,062 + EX28DRAFT_1908 0.52 +0.3 1,917,062 + EX28DRAFT_1908 0.52 +0.2 1,917,163 - EX28DRAFT_1908 0.61 -0.2 1,917,165 - EX28DRAFT_1908 0.61 +0.3 1,917,259 + EX28DRAFT_1908 0.69 +0.4 1,917,260 - EX28DRAFT_1908 0.69 -1.9 1,917,292 - EX28DRAFT_1908 0.72 -0.6 1,917,292 - EX28DRAFT_1908 0.72 -1.2 1,917,295 + EX28DRAFT_1908 0.72 +1.6 1,917,296 - EX28DRAFT_1908 0.72 -0.1 1,917,296 - EX28DRAFT_1908 0.72 +0.7 1,917,296 - EX28DRAFT_1908 0.72 +0.7 1,917,296 - EX28DRAFT_1908 0.72 -1.0 1,917,356 + EX28DRAFT_1908 0.77 +0.4 1,917,356 + EX28DRAFT_1908 0.77 -3.2 1,917,357 - EX28DRAFT_1908 0.77 +1.0 1,917,357 - EX28DRAFT_1908 0.77 -0.4 1,917,676 + -0.4 1,917,676 + -0.5 1,917,677 - +0.1 1,917,677 - -0.3 1,917,900 + EX28DRAFT_1909 0.46 -1.1 1,917,994 - EX28DRAFT_1909 0.68 -0.4 1,918,092 + -0.2 1,918,147 + +0.9 1,918,147 + -0.4 1,918,148 - -0.4 1,918,148 - +0.7 1,918,149 + -0.6 1,918,150 - +3.3 1,918,150 - +1.3 1,918,241 + -0.1 1,918,245 + +0.3 1,918,246 - +0.4 1,918,282 - -0.1 1,918,622 - EX28DRAFT_1910 0.25 +0.7 1,918,622 - EX28DRAFT_1910 0.25 -0.1 1,918,681 - EX28DRAFT_1910 0.29 +1.5 1,918,931 - EX28DRAFT_1910 0.46 +0.4 1,919,011 - EX28DRAFT_1910 0.51 -0.1
Or see this region's nucleotide sequence