Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1361 and EX28DRAFT_1362 are separated by 10 nucleotides EX28DRAFT_1362 and EX28DRAFT_1363 are separated by 213 nucleotides EX28DRAFT_1363 and EX28DRAFT_1364 are separated by 23 nucleotides
EX28DRAFT_1361: EX28DRAFT_1361 - hypothetical protein, at 1,379,154 to 1,380,269
_1361
EX28DRAFT_1362: EX28DRAFT_1362 - hypothetical protein, at 1,380,280 to 1,380,963
_1362
EX28DRAFT_1363: EX28DRAFT_1363 - Domain of unknown function (DUF1852), at 1,381,177 to 1,382,151
_1363
EX28DRAFT_1364: EX28DRAFT_1364 - Methionine synthase II (cobalamin-independent), at 1,382,175 to 1,383,203
_1364
Position (kb)
1381
1382
1383 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1380.207 kb on - strand at 1380.219 kb on + strand at 1380.257 kb on - strand at 1380.257 kb on - strand at 1380.275 kb on + strand at 1380.296 kb on + strand at 1380.352 kb on - strand, within EX28DRAFT_1362 at 1380.352 kb on - strand, within EX28DRAFT_1362 at 1380.461 kb on + strand, within EX28DRAFT_1362 at 1380.484 kb on - strand, within EX28DRAFT_1362 at 1380.489 kb on + strand, within EX28DRAFT_1362 at 1380.510 kb on + strand, within EX28DRAFT_1362 at 1380.510 kb on + strand, within EX28DRAFT_1362 at 1380.546 kb on - strand, within EX28DRAFT_1362 at 1380.608 kb on - strand, within EX28DRAFT_1362 at 1380.647 kb on - strand, within EX28DRAFT_1362 at 1380.726 kb on + strand, within EX28DRAFT_1362 at 1380.749 kb on - strand, within EX28DRAFT_1362 at 1380.749 kb on - strand, within EX28DRAFT_1362 at 1380.767 kb on + strand, within EX28DRAFT_1362 at 1380.785 kb on - strand, within EX28DRAFT_1362 at 1380.813 kb on - strand, within EX28DRAFT_1362 at 1380.831 kb on + strand, within EX28DRAFT_1362 at 1380.831 kb on + strand, within EX28DRAFT_1362 at 1380.832 kb on - strand, within EX28DRAFT_1362 at 1380.832 kb on - strand, within EX28DRAFT_1362 at 1380.882 kb on + strand, within EX28DRAFT_1362 at 1380.898 kb on + strand at 1380.898 kb on + strand at 1380.918 kb on + strand at 1380.918 kb on + strand at 1380.951 kb on + strand at 1381.044 kb on - strand at 1381.128 kb on + strand at 1381.195 kb on + strand at 1381.196 kb on - strand at 1381.196 kb on - strand at 1381.196 kb on - strand at 1381.196 kb on - strand at 1381.197 kb on + strand at 1381.197 kb on + strand at 1381.197 kb on + strand at 1381.228 kb on + strand at 1381.228 kb on + strand at 1381.311 kb on + strand, within EX28DRAFT_1363 at 1381.513 kb on - strand, within EX28DRAFT_1363 at 1381.525 kb on + strand, within EX28DRAFT_1363 at 1381.789 kb on + strand, within EX28DRAFT_1363 at 1381.985 kb on - strand, within EX28DRAFT_1363 at 1382.036 kb on - strand, within EX28DRAFT_1363 at 1382.092 kb on + strand at 1382.093 kb on - strand at 1382.140 kb on + strand at 1382.140 kb on + strand at 1382.141 kb on - strand at 1382.141 kb on - strand at 1382.141 kb on - strand at 1382.141 kb on - strand at 1382.150 kb on - strand at 1382.171 kb on + strand at 1382.171 kb on + strand at 1382.171 kb on + strand at 1382.172 kb on - strand at 1382.393 kb on + strand, within EX28DRAFT_1364 at 1382.394 kb on - strand, within EX28DRAFT_1364 at 1382.610 kb on + strand, within EX28DRAFT_1364 at 1382.613 kb on - strand, within EX28DRAFT_1364 at 1382.613 kb on - strand, within EX28DRAFT_1364 at 1382.674 kb on + strand, within EX28DRAFT_1364 at 1382.921 kb on + strand, within EX28DRAFT_1364 at 1382.921 kb on + strand, within EX28DRAFT_1364 at 1382.982 kb on - strand, within EX28DRAFT_1364 at 1383.059 kb on + strand, within EX28DRAFT_1364
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 1,380,207 - -0.2 1,380,219 + -0.2 1,380,257 - -2.5 1,380,257 - +0.2 1,380,275 + +1.5 1,380,296 + -0.7 1,380,352 - EX28DRAFT_1362 0.11 -2.1 1,380,352 - EX28DRAFT_1362 0.11 +1.6 1,380,461 + EX28DRAFT_1362 0.26 -0.7 1,380,484 - EX28DRAFT_1362 0.30 +0.4 1,380,489 + EX28DRAFT_1362 0.31 -0.4 1,380,510 + EX28DRAFT_1362 0.34 -2.3 1,380,510 + EX28DRAFT_1362 0.34 -3.7 1,380,546 - EX28DRAFT_1362 0.39 +1.6 1,380,608 - EX28DRAFT_1362 0.48 -0.9 1,380,647 - EX28DRAFT_1362 0.54 -1.3 1,380,726 + EX28DRAFT_1362 0.65 -0.3 1,380,749 - EX28DRAFT_1362 0.69 -0.7 1,380,749 - EX28DRAFT_1362 0.69 -4.0 1,380,767 + EX28DRAFT_1362 0.71 -0.2 1,380,785 - EX28DRAFT_1362 0.74 +0.1 1,380,813 - EX28DRAFT_1362 0.78 +0.8 1,380,831 + EX28DRAFT_1362 0.81 -0.5 1,380,831 + EX28DRAFT_1362 0.81 -2.2 1,380,832 - EX28DRAFT_1362 0.81 -0.2 1,380,832 - EX28DRAFT_1362 0.81 -0.1 1,380,882 + EX28DRAFT_1362 0.88 +0.3 1,380,898 + +0.5 1,380,898 + -0.5 1,380,918 + -0.0 1,380,918 + -0.4 1,380,951 + +1.4 1,381,044 - -0.7 1,381,128 + +0.2 1,381,195 + -2.0 1,381,196 - -0.7 1,381,196 - -0.7 1,381,196 - -0.6 1,381,196 - -0.4 1,381,197 + -0.5 1,381,197 + +1.7 1,381,197 + -3.1 1,381,228 + -1.1 1,381,228 + -0.9 1,381,311 + EX28DRAFT_1363 0.14 -0.2 1,381,513 - EX28DRAFT_1363 0.34 -0.7 1,381,525 + EX28DRAFT_1363 0.36 -0.9 1,381,789 + EX28DRAFT_1363 0.63 -0.8 1,381,985 - EX28DRAFT_1363 0.83 +0.3 1,382,036 - EX28DRAFT_1363 0.88 -1.3 1,382,092 + -0.7 1,382,093 - -1.4 1,382,140 + -2.7 1,382,140 + +0.3 1,382,141 - -0.3 1,382,141 - -0.1 1,382,141 - +2.0 1,382,141 - -0.3 1,382,150 - -1.0 1,382,171 + -0.6 1,382,171 + -3.1 1,382,171 + +0.0 1,382,172 - -1.0 1,382,393 + EX28DRAFT_1364 0.21 -2.0 1,382,394 - EX28DRAFT_1364 0.21 -0.4 1,382,610 + EX28DRAFT_1364 0.42 -0.3 1,382,613 - EX28DRAFT_1364 0.43 -1.5 1,382,613 - EX28DRAFT_1364 0.43 -0.4 1,382,674 + EX28DRAFT_1364 0.48 +1.8 1,382,921 + EX28DRAFT_1364 0.72 +0.3 1,382,921 + EX28DRAFT_1364 0.72 -1.1 1,382,982 - EX28DRAFT_1364 0.78 -0.7 1,383,059 + EX28DRAFT_1364 0.86 -1.0
Or see this region's nucleotide sequence