Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1238 and EX28DRAFT_1239 are separated by 236 nucleotides EX28DRAFT_1239 and EX28DRAFT_1240 are separated by 102 nucleotides EX28DRAFT_1240 and EX28DRAFT_1241 are separated by 94 nucleotides EX28DRAFT_1241 and EX28DRAFT_1242 are separated by 23 nucleotides
EX28DRAFT_1238: EX28DRAFT_1238 - Uncharacterized protein conserved in bacteria, at 1,257,459 to 1,258,802
_1238
EX28DRAFT_1239: EX28DRAFT_1239 - hypothetical protein, at 1,259,039 to 1,259,227
_1239
EX28DRAFT_1240: EX28DRAFT_1240 - Stress-induced bacterial acidophilic repeat motif, at 1,259,330 to 1,259,515
_1240
EX28DRAFT_1241: EX28DRAFT_1241 - Uncharacterized protein conserved in bacteria, at 1,259,610 to 1,260,103
_1241
EX28DRAFT_1242: EX28DRAFT_1242 - hypothetical protein, at 1,260,127 to 1,260,330
_1242
Position (kb)
1259
1260 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1258.078 kb on + strand, within EX28DRAFT_1238 at 1258.079 kb on - strand, within EX28DRAFT_1238 at 1258.079 kb on - strand, within EX28DRAFT_1238 at 1258.140 kb on - strand, within EX28DRAFT_1238 at 1258.201 kb on + strand, within EX28DRAFT_1238 at 1258.201 kb on + strand, within EX28DRAFT_1238 at 1258.287 kb on - strand, within EX28DRAFT_1238 at 1258.513 kb on + strand, within EX28DRAFT_1238 at 1258.514 kb on - strand, within EX28DRAFT_1238 at 1258.514 kb on - strand, within EX28DRAFT_1238 at 1258.514 kb on - strand, within EX28DRAFT_1238 at 1258.516 kb on + strand, within EX28DRAFT_1238 at 1258.516 kb on + strand, within EX28DRAFT_1238 at 1258.550 kb on + strand, within EX28DRAFT_1238 at 1258.550 kb on + strand, within EX28DRAFT_1238 at 1258.654 kb on - strand, within EX28DRAFT_1238 at 1258.654 kb on - strand, within EX28DRAFT_1238 at 1258.656 kb on + strand, within EX28DRAFT_1238 at 1258.656 kb on + strand, within EX28DRAFT_1238 at 1258.656 kb on + strand, within EX28DRAFT_1238 at 1258.657 kb on - strand, within EX28DRAFT_1238 at 1258.664 kb on + strand, within EX28DRAFT_1238 at 1258.665 kb on - strand, within EX28DRAFT_1238 at 1258.665 kb on - strand, within EX28DRAFT_1238 at 1258.686 kb on + strand at 1258.687 kb on - strand at 1258.687 kb on - strand at 1258.687 kb on - strand at 1258.718 kb on - strand at 1258.738 kb on + strand at 1258.738 kb on + strand at 1258.752 kb on - strand at 1258.752 kb on - strand at 1258.824 kb on - strand at 1258.826 kb on + strand at 1259.153 kb on - strand, within EX28DRAFT_1239 at 1259.294 kb on + strand at 1259.309 kb on + strand at 1259.309 kb on + strand at 1259.312 kb on - strand at 1259.587 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 1,258,078 + EX28DRAFT_1238 0.46 -0.4 1,258,079 - EX28DRAFT_1238 0.46 +0.7 1,258,079 - EX28DRAFT_1238 0.46 +0.2 1,258,140 - EX28DRAFT_1238 0.51 -0.2 1,258,201 + EX28DRAFT_1238 0.55 +0.5 1,258,201 + EX28DRAFT_1238 0.55 -0.3 1,258,287 - EX28DRAFT_1238 0.62 -1.7 1,258,513 + EX28DRAFT_1238 0.78 +0.5 1,258,514 - EX28DRAFT_1238 0.78 -1.1 1,258,514 - EX28DRAFT_1238 0.78 -0.6 1,258,514 - EX28DRAFT_1238 0.78 +1.0 1,258,516 + EX28DRAFT_1238 0.79 -0.9 1,258,516 + EX28DRAFT_1238 0.79 -1.3 1,258,550 + EX28DRAFT_1238 0.81 +2.8 1,258,550 + EX28DRAFT_1238 0.81 -2.3 1,258,654 - EX28DRAFT_1238 0.89 -0.6 1,258,654 - EX28DRAFT_1238 0.89 +0.7 1,258,656 + EX28DRAFT_1238 0.89 -1.4 1,258,656 + EX28DRAFT_1238 0.89 -1.3 1,258,656 + EX28DRAFT_1238 0.89 +0.6 1,258,657 - EX28DRAFT_1238 0.89 -0.4 1,258,664 + EX28DRAFT_1238 0.90 +2.2 1,258,665 - EX28DRAFT_1238 0.90 -0.8 1,258,665 - EX28DRAFT_1238 0.90 -0.7 1,258,686 + +0.6 1,258,687 - +1.3 1,258,687 - -0.7 1,258,687 - -0.3 1,258,718 - -0.1 1,258,738 + -2.1 1,258,738 + -1.3 1,258,752 - +0.6 1,258,752 - -0.0 1,258,824 - -2.1 1,258,826 + -0.6 1,259,153 - EX28DRAFT_1239 0.60 -1.0 1,259,294 + -2.5 1,259,309 + -3.0 1,259,309 + +0.2 1,259,312 - +1.0 1,259,587 + +0.3
Or see this region's nucleotide sequence