Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0933 and EX28DRAFT_0934 are separated by 10 nucleotides EX28DRAFT_0934 and EX28DRAFT_0935 are separated by 3 nucleotides
EX28DRAFT_0933: EX28DRAFT_0933 - tryptophan synthase, beta subunit, at 931,491 to 932,684
_0933
EX28DRAFT_0934: EX28DRAFT_0934 - indole-3-glycerol phosphate synthase (EC 4.1.1.48), at 932,695 to 934,053
_0934
EX28DRAFT_0935: EX28DRAFT_0935 - glutamine amidotransferase of anthranilate synthase or aminodeoxychorismate synthase, at 934,057 to 935,652
_0935
Position (kb) 932
933
934
935 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 931.714 kb on + strand, within EX28DRAFT_0933 at 931.715 kb on - strand, within EX28DRAFT_0933 at 931.792 kb on + strand, within EX28DRAFT_0933 at 931.792 kb on + strand, within EX28DRAFT_0933 at 931.793 kb on - strand, within EX28DRAFT_0933 at 931.849 kb on + strand, within EX28DRAFT_0933 at 932.127 kb on - strand, within EX28DRAFT_0933 at 932.127 kb on - strand, within EX28DRAFT_0933 at 932.127 kb on - strand, within EX28DRAFT_0933 at 932.128 kb on + strand, within EX28DRAFT_0933 at 932.128 kb on + strand, within EX28DRAFT_0933 at 932.128 kb on + strand, within EX28DRAFT_0933 at 932.129 kb on - strand, within EX28DRAFT_0933 at 932.129 kb on - strand, within EX28DRAFT_0933 at 932.129 kb on - strand, within EX28DRAFT_0933 at 932.129 kb on - strand, within EX28DRAFT_0933 at 932.129 kb on - strand, within EX28DRAFT_0933 at 932.129 kb on - strand, within EX28DRAFT_0933 at 932.143 kb on + strand, within EX28DRAFT_0933 at 932.143 kb on + strand, within EX28DRAFT_0933 at 932.144 kb on - strand, within EX28DRAFT_0933 at 932.287 kb on + strand, within EX28DRAFT_0933 at 932.287 kb on + strand, within EX28DRAFT_0933 at 932.287 kb on + strand, within EX28DRAFT_0933 at 932.288 kb on - strand, within EX28DRAFT_0933 at 932.427 kb on - strand, within EX28DRAFT_0933 at 932.464 kb on + strand, within EX28DRAFT_0933 at 932.638 kb on + strand at 932.639 kb on - strand at 932.639 kb on - strand at 932.639 kb on - strand at 932.673 kb on + strand at 932.673 kb on + strand at 932.674 kb on - strand at 932.697 kb on - strand at 932.700 kb on - strand at 932.749 kb on - strand at 932.764 kb on + strand at 932.812 kb on + strand at 932.813 kb on - strand at 932.830 kb on + strand at 932.927 kb on + strand, within EX28DRAFT_0934 at 932.927 kb on + strand, within EX28DRAFT_0934 at 932.928 kb on - strand, within EX28DRAFT_0934 at 932.931 kb on + strand, within EX28DRAFT_0934 at 932.931 kb on + strand, within EX28DRAFT_0934 at 932.932 kb on - strand, within EX28DRAFT_0934 at 932.964 kb on + strand, within EX28DRAFT_0934 at 932.965 kb on - strand, within EX28DRAFT_0934 at 933.032 kb on + strand, within EX28DRAFT_0934 at 933.033 kb on - strand, within EX28DRAFT_0934 at 933.108 kb on + strand, within EX28DRAFT_0934 at 933.108 kb on + strand, within EX28DRAFT_0934 at 933.108 kb on + strand, within EX28DRAFT_0934 at 933.134 kb on + strand, within EX28DRAFT_0934 at 933.134 kb on + strand, within EX28DRAFT_0934 at 933.134 kb on + strand, within EX28DRAFT_0934 at 933.135 kb on - strand, within EX28DRAFT_0934 at 933.135 kb on - strand, within EX28DRAFT_0934 at 933.163 kb on - strand, within EX28DRAFT_0934 at 933.163 kb on - strand, within EX28DRAFT_0934 at 933.377 kb on - strand, within EX28DRAFT_0934 at 933.377 kb on - strand, within EX28DRAFT_0934 at 933.669 kb on - strand, within EX28DRAFT_0934 at 933.669 kb on - strand, within EX28DRAFT_0934 at 933.702 kb on + strand, within EX28DRAFT_0934 at 933.706 kb on - strand, within EX28DRAFT_0934 at 933.824 kb on + strand, within EX28DRAFT_0934 at 933.935 kb on + strand at 934.111 kb on + strand at 934.112 kb on - strand at 934.112 kb on - strand at 934.112 kb on - strand at 934.228 kb on + strand, within EX28DRAFT_0935 at 934.290 kb on - strand, within EX28DRAFT_0935 at 934.290 kb on - strand, within EX28DRAFT_0935 at 934.326 kb on - strand, within EX28DRAFT_0935 at 934.326 kb on - strand, within EX28DRAFT_0935 at 934.596 kb on - strand, within EX28DRAFT_0935 at 934.662 kb on + strand, within EX28DRAFT_0935 at 934.674 kb on + strand, within EX28DRAFT_0935 at 934.789 kb on - strand, within EX28DRAFT_0935 at 934.801 kb on - strand, within EX28DRAFT_0935 at 934.828 kb on - strand, within EX28DRAFT_0935
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 931,714 + EX28DRAFT_0933 0.19 -5.4 931,715 - EX28DRAFT_0933 0.19 -4.5 931,792 + EX28DRAFT_0933 0.25 -3.1 931,792 + EX28DRAFT_0933 0.25 -3.9 931,793 - EX28DRAFT_0933 0.25 -3.2 931,849 + EX28DRAFT_0933 0.30 -6.4 932,127 - EX28DRAFT_0933 0.53 -1.6 932,127 - EX28DRAFT_0933 0.53 -1.6 932,127 - EX28DRAFT_0933 0.53 -2.8 932,128 + EX28DRAFT_0933 0.53 -4.3 932,128 + EX28DRAFT_0933 0.53 -4.6 932,128 + EX28DRAFT_0933 0.53 -5.2 932,129 - EX28DRAFT_0933 0.53 -2.6 932,129 - EX28DRAFT_0933 0.53 -4.5 932,129 - EX28DRAFT_0933 0.53 -4.2 932,129 - EX28DRAFT_0933 0.53 -4.8 932,129 - EX28DRAFT_0933 0.53 -2.7 932,129 - EX28DRAFT_0933 0.53 -1.6 932,143 + EX28DRAFT_0933 0.55 -3.4 932,143 + EX28DRAFT_0933 0.55 -4.0 932,144 - EX28DRAFT_0933 0.55 -3.4 932,287 + EX28DRAFT_0933 0.67 -1.9 932,287 + EX28DRAFT_0933 0.67 -3.0 932,287 + EX28DRAFT_0933 0.67 -2.8 932,288 - EX28DRAFT_0933 0.67 -5.2 932,427 - EX28DRAFT_0933 0.78 -4.6 932,464 + EX28DRAFT_0933 0.81 -1.4 932,638 + -4.9 932,639 - -2.9 932,639 - -2.9 932,639 - -3.9 932,673 + -4.6 932,673 + -2.1 932,674 - -4.4 932,697 - -0.2 932,700 - +0.6 932,749 - -4.3 932,764 + -3.6 932,812 + -2.6 932,813 - -3.3 932,830 + -1.8 932,927 + EX28DRAFT_0934 0.17 -4.0 932,927 + EX28DRAFT_0934 0.17 -4.8 932,928 - EX28DRAFT_0934 0.17 -4.0 932,931 + EX28DRAFT_0934 0.17 -5.7 932,931 + EX28DRAFT_0934 0.17 -4.1 932,932 - EX28DRAFT_0934 0.17 -4.5 932,964 + EX28DRAFT_0934 0.20 -3.1 932,965 - EX28DRAFT_0934 0.20 -4.9 933,032 + EX28DRAFT_0934 0.25 -6.1 933,033 - EX28DRAFT_0934 0.25 -5.9 933,108 + EX28DRAFT_0934 0.30 -6.3 933,108 + EX28DRAFT_0934 0.30 -4.5 933,108 + EX28DRAFT_0934 0.30 -0.6 933,134 + EX28DRAFT_0934 0.32 -3.7 933,134 + EX28DRAFT_0934 0.32 -2.1 933,134 + EX28DRAFT_0934 0.32 -6.4 933,135 - EX28DRAFT_0934 0.32 -4.0 933,135 - EX28DRAFT_0934 0.32 -3.7 933,163 - EX28DRAFT_0934 0.34 -4.4 933,163 - EX28DRAFT_0934 0.34 -5.9 933,377 - EX28DRAFT_0934 0.50 -4.5 933,377 - EX28DRAFT_0934 0.50 -1.6 933,669 - EX28DRAFT_0934 0.72 -0.6 933,669 - EX28DRAFT_0934 0.72 -4.3 933,702 + EX28DRAFT_0934 0.74 -2.1 933,706 - EX28DRAFT_0934 0.74 -6.1 933,824 + EX28DRAFT_0934 0.83 -2.9 933,935 + -0.6 934,111 + -0.6 934,112 - -2.6 934,112 - -2.0 934,112 - -5.1 934,228 + EX28DRAFT_0935 0.11 -3.4 934,290 - EX28DRAFT_0935 0.15 -3.7 934,290 - EX28DRAFT_0935 0.15 -2.6 934,326 - EX28DRAFT_0935 0.17 -2.9 934,326 - EX28DRAFT_0935 0.17 -4.3 934,596 - EX28DRAFT_0935 0.34 -1.9 934,662 + EX28DRAFT_0935 0.38 -4.8 934,674 + EX28DRAFT_0935 0.39 -1.6 934,789 - EX28DRAFT_0935 0.46 -1.8 934,801 - EX28DRAFT_0935 0.47 -3.4 934,828 - EX28DRAFT_0935 0.48 -2.5
Or see this region's nucleotide sequence