Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0920 and EX28DRAFT_0921 are separated by 41 nucleotides EX28DRAFT_0921 and EX28DRAFT_0922 are separated by 205 nucleotides EX28DRAFT_0922 and EX28DRAFT_0923 are separated by 38 nucleotides EX28DRAFT_0923 and EX28DRAFT_0924 are separated by 104 nucleotides
EX28DRAFT_0920: EX28DRAFT_0920 - Transcriptional regulators, at 921,883 to 922,836
_0920
EX28DRAFT_0921: EX28DRAFT_0921 - Uncharacterized protein conserved in bacteria, at 922,878 to 923,174
_0921
EX28DRAFT_0922: EX28DRAFT_0922 - TonB family C-terminal domain, at 923,380 to 924,117
_0922
EX28DRAFT_0923: EX28DRAFT_0923 - Acyl-CoA hydrolase, at 924,156 to 924,551
_0923
EX28DRAFT_0924: EX28DRAFT_0924 - intracellular septation protein A, at 924,656 to 925,195
_0924
Position (kb)
923
924
925 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 922.412 kb on - strand, within EX28DRAFT_0920 at 922.479 kb on + strand, within EX28DRAFT_0920 at 922.602 kb on + strand, within EX28DRAFT_0920 at 922.714 kb on - strand, within EX28DRAFT_0920 at 922.834 kb on + strand at 922.841 kb on + strand at 922.893 kb on - strand at 922.894 kb on + strand at 923.065 kb on - strand, within EX28DRAFT_0921 at 923.207 kb on + strand at 923.207 kb on + strand at 923.208 kb on - strand at 923.213 kb on - strand at 923.240 kb on - strand at 923.260 kb on - strand at 923.260 kb on - strand at 923.386 kb on - strand at 923.911 kb on - strand, within EX28DRAFT_0922 at 923.975 kb on + strand, within EX28DRAFT_0922 at 923.996 kb on + strand, within EX28DRAFT_0922 at 923.998 kb on - strand, within EX28DRAFT_0922 at 924.196 kb on - strand, within EX28DRAFT_0923 at 924.196 kb on - strand, within EX28DRAFT_0923 at 924.206 kb on - strand, within EX28DRAFT_0923 at 924.209 kb on + strand, within EX28DRAFT_0923 at 924.209 kb on + strand, within EX28DRAFT_0923 at 924.210 kb on - strand, within EX28DRAFT_0923 at 924.230 kb on + strand, within EX28DRAFT_0923 at 924.230 kb on + strand, within EX28DRAFT_0923 at 924.232 kb on + strand, within EX28DRAFT_0923 at 924.267 kb on - strand, within EX28DRAFT_0923 at 924.294 kb on - strand, within EX28DRAFT_0923 at 924.294 kb on - strand, within EX28DRAFT_0923 at 924.294 kb on - strand, within EX28DRAFT_0923 at 924.294 kb on - strand, within EX28DRAFT_0923 at 924.438 kb on - strand, within EX28DRAFT_0923 at 924.558 kb on - strand at 924.589 kb on + strand at 924.589 kb on + strand at 924.590 kb on - strand at 924.615 kb on - strand at 924.679 kb on + strand at 924.687 kb on + strand at 924.688 kb on - strand at 924.688 kb on - strand at 924.693 kb on + strand at 924.693 kb on + strand at 924.693 kb on + strand at 924.694 kb on - strand at 924.719 kb on - strand, within EX28DRAFT_0924
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 922,412 - EX28DRAFT_0920 0.55 +0.5 922,479 + EX28DRAFT_0920 0.62 -0.5 922,602 + EX28DRAFT_0920 0.75 +0.4 922,714 - EX28DRAFT_0920 0.87 +1.6 922,834 + -2.5 922,841 + -0.2 922,893 - -1.1 922,894 + -1.4 923,065 - EX28DRAFT_0921 0.63 +0.8 923,207 + -0.6 923,207 + +0.5 923,208 - +0.5 923,213 - -1.1 923,240 - -0.4 923,260 - -0.5 923,260 - -1.0 923,386 - +0.5 923,911 - EX28DRAFT_0922 0.72 -3.4 923,975 + EX28DRAFT_0922 0.81 -2.1 923,996 + EX28DRAFT_0922 0.83 -0.5 923,998 - EX28DRAFT_0922 0.84 -3.1 924,196 - EX28DRAFT_0923 0.10 +0.9 924,196 - EX28DRAFT_0923 0.10 +0.3 924,206 - EX28DRAFT_0923 0.13 +0.0 924,209 + EX28DRAFT_0923 0.13 -0.5 924,209 + EX28DRAFT_0923 0.13 +0.2 924,210 - EX28DRAFT_0923 0.14 -0.9 924,230 + EX28DRAFT_0923 0.19 +0.5 924,230 + EX28DRAFT_0923 0.19 -0.9 924,232 + EX28DRAFT_0923 0.19 +0.2 924,267 - EX28DRAFT_0923 0.28 -1.8 924,294 - EX28DRAFT_0923 0.35 +0.3 924,294 - EX28DRAFT_0923 0.35 -0.2 924,294 - EX28DRAFT_0923 0.35 +0.6 924,294 - EX28DRAFT_0923 0.35 +0.7 924,438 - EX28DRAFT_0923 0.71 +0.1 924,558 - -0.1 924,589 + +1.2 924,589 + +1.3 924,590 - +0.6 924,615 - +0.0 924,679 + -0.0 924,687 + -0.4 924,688 - -1.1 924,688 - -0.2 924,693 + -2.5 924,693 + -1.1 924,693 + -0.8 924,694 - -0.1 924,719 - EX28DRAFT_0924 0.12 -0.5
Or see this region's nucleotide sequence