Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0879 and EX28DRAFT_0880 are separated by 120 nucleotides EX28DRAFT_0880 and EX28DRAFT_0881 are separated by 41 nucleotides EX28DRAFT_0881 and EX28DRAFT_0882 are separated by 2 nucleotides EX28DRAFT_0882 and EX28DRAFT_0883 are separated by 97 nucleotides EX28DRAFT_0883 and EX28DRAFT_0884 overlap by 4 nucleotides EX28DRAFT_0884 and EX28DRAFT_0885 are separated by 66 nucleotides EX28DRAFT_0885 and EX28DRAFT_0886 are separated by 31 nucleotides
EX28DRAFT_0879: EX28DRAFT_0879 - Helix-turn-helix, at 891,403 to 891,702
_0879
EX28DRAFT_0880: EX28DRAFT_0880 - Regulatory phage protein cox, at 891,823 to 892,101
_0880
EX28DRAFT_0881: EX28DRAFT_0881 - hypothetical protein, at 892,143 to 892,382
_0881
EX28DRAFT_0882: EX28DRAFT_0882 - hypothetical protein, at 892,385 to 892,573
_0882
EX28DRAFT_0883: EX28DRAFT_0883 - hypothetical protein, at 892,671 to 892,841
_0883
EX28DRAFT_0884: EX28DRAFT_0884 - hypothetical protein, at 892,838 to 893,338
_0884
EX28DRAFT_0885: EX28DRAFT_0885 - Protein of unknown function (DUF2732), at 893,405 to 893,614
_0885
EX28DRAFT_0886: EX28DRAFT_0886 - hypothetical protein, at 893,646 to 893,909
_0886
Position (kb)
892
893 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 891.786 kb on - strand at 891.796 kb on - strand at 891.817 kb on + strand at 891.839 kb on + strand at 891.840 kb on - strand at 891.840 kb on - strand at 891.840 kb on - strand at 891.840 kb on - strand at 891.840 kb on - strand at 891.840 kb on - strand at 891.843 kb on + strand at 891.843 kb on + strand at 891.882 kb on + strand, within EX28DRAFT_0880 at 891.888 kb on + strand, within EX28DRAFT_0880 at 891.889 kb on - strand, within EX28DRAFT_0880 at 891.889 kb on - strand, within EX28DRAFT_0880 at 891.985 kb on + strand, within EX28DRAFT_0880 at 891.994 kb on + strand, within EX28DRAFT_0880 at 891.994 kb on + strand, within EX28DRAFT_0880 at 892.030 kb on + strand, within EX28DRAFT_0880 at 892.178 kb on + strand, within EX28DRAFT_0881 at 892.179 kb on - strand, within EX28DRAFT_0881 at 892.190 kb on + strand, within EX28DRAFT_0881 at 892.326 kb on + strand, within EX28DRAFT_0881 at 892.327 kb on - strand, within EX28DRAFT_0881 at 892.376 kb on + strand at 892.377 kb on - strand at 892.377 kb on - strand at 892.386 kb on + strand at 892.386 kb on + strand at 892.387 kb on - strand at 892.387 kb on - strand at 892.387 kb on - strand at 892.394 kb on - strand at 892.408 kb on + strand, within EX28DRAFT_0882 at 892.426 kb on + strand, within EX28DRAFT_0882 at 892.426 kb on + strand, within EX28DRAFT_0882 at 892.513 kb on + strand, within EX28DRAFT_0882 at 892.547 kb on + strand, within EX28DRAFT_0882 at 892.568 kb on - strand at 892.568 kb on - strand at 892.578 kb on + strand at 892.578 kb on + strand at 892.644 kb on + strand at 892.671 kb on - strand at 892.766 kb on - strand, within EX28DRAFT_0883 at 892.766 kb on - strand, within EX28DRAFT_0883 at 892.904 kb on + strand, within EX28DRAFT_0884 at 892.905 kb on - strand, within EX28DRAFT_0884 at 892.996 kb on - strand, within EX28DRAFT_0884 at 893.197 kb on + strand, within EX28DRAFT_0884 at 893.198 kb on - strand, within EX28DRAFT_0884 at 893.296 kb on + strand at 893.297 kb on - strand at 893.305 kb on - strand at 893.327 kb on + strand at 893.332 kb on - strand at 893.336 kb on + strand at 893.399 kb on - strand at 893.527 kb on + strand, within EX28DRAFT_0885 at 893.569 kb on - strand, within EX28DRAFT_0885 at 893.569 kb on - strand, within EX28DRAFT_0885 at 893.572 kb on - strand, within EX28DRAFT_0885 at 893.572 kb on - strand, within EX28DRAFT_0885 at 893.572 kb on - strand, within EX28DRAFT_0885 at 893.653 kb on + strand at 893.666 kb on + strand at 893.676 kb on + strand, within EX28DRAFT_0886 at 893.677 kb on - strand, within EX28DRAFT_0886 at 893.798 kb on + strand, within EX28DRAFT_0886 at 893.798 kb on + strand, within EX28DRAFT_0886 at 893.798 kb on + strand, within EX28DRAFT_0886 at 893.798 kb on + strand, within EX28DRAFT_0886 at 893.798 kb on + strand, within EX28DRAFT_0886 at 893.799 kb on - strand, within EX28DRAFT_0886
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 891,786 - +0.1 891,796 - -1.7 891,817 + -0.2 891,839 + +0.9 891,840 - +0.6 891,840 - -0.1 891,840 - -0.5 891,840 - +0.3 891,840 - -0.7 891,840 - -0.1 891,843 + -0.5 891,843 + +0.2 891,882 + EX28DRAFT_0880 0.21 +0.0 891,888 + EX28DRAFT_0880 0.23 +2.4 891,889 - EX28DRAFT_0880 0.24 +0.1 891,889 - EX28DRAFT_0880 0.24 -0.0 891,985 + EX28DRAFT_0880 0.58 +0.9 891,994 + EX28DRAFT_0880 0.61 -4.4 891,994 + EX28DRAFT_0880 0.61 +0.4 892,030 + EX28DRAFT_0880 0.74 +1.0 892,178 + EX28DRAFT_0881 0.15 +1.1 892,179 - EX28DRAFT_0881 0.15 -0.2 892,190 + EX28DRAFT_0881 0.20 +0.3 892,326 + EX28DRAFT_0881 0.76 +1.4 892,327 - EX28DRAFT_0881 0.77 -0.2 892,376 + -0.3 892,377 - +0.1 892,377 - +0.6 892,386 + -1.0 892,386 + -0.1 892,387 - -4.3 892,387 - -0.3 892,387 - -0.4 892,394 - -1.3 892,408 + EX28DRAFT_0882 0.12 +1.1 892,426 + EX28DRAFT_0882 0.22 -0.0 892,426 + EX28DRAFT_0882 0.22 +0.3 892,513 + EX28DRAFT_0882 0.68 -0.4 892,547 + EX28DRAFT_0882 0.86 +1.2 892,568 - -1.1 892,568 - +1.3 892,578 + -0.4 892,578 + +0.2 892,644 + -0.5 892,671 - +2.7 892,766 - EX28DRAFT_0883 0.56 +0.1 892,766 - EX28DRAFT_0883 0.56 -1.5 892,904 + EX28DRAFT_0884 0.13 +0.4 892,905 - EX28DRAFT_0884 0.13 -0.3 892,996 - EX28DRAFT_0884 0.32 +1.7 893,197 + EX28DRAFT_0884 0.72 +2.3 893,198 - EX28DRAFT_0884 0.72 -0.7 893,296 + -0.5 893,297 - +0.0 893,305 - -0.8 893,327 + +0.1 893,332 - -0.2 893,336 + +0.5 893,399 - -0.7 893,527 + EX28DRAFT_0885 0.58 +1.1 893,569 - EX28DRAFT_0885 0.78 -0.8 893,569 - EX28DRAFT_0885 0.78 +0.8 893,572 - EX28DRAFT_0885 0.80 -0.7 893,572 - EX28DRAFT_0885 0.80 +0.8 893,572 - EX28DRAFT_0885 0.80 +0.8 893,653 + -2.5 893,666 + +0.4 893,676 + EX28DRAFT_0886 0.11 -0.2 893,677 - EX28DRAFT_0886 0.12 -1.7 893,798 + EX28DRAFT_0886 0.58 -2.3 893,798 + EX28DRAFT_0886 0.58 -0.5 893,798 + EX28DRAFT_0886 0.58 -0.2 893,798 + EX28DRAFT_0886 0.58 -0.6 893,798 + EX28DRAFT_0886 0.58 -2.6 893,799 - EX28DRAFT_0886 0.58 +0.1
Or see this region's nucleotide sequence