Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0814 and EX28DRAFT_0815 overlap by 8 nucleotides EX28DRAFT_0815 and EX28DRAFT_0816 are separated by 71 nucleotides EX28DRAFT_0816 and EX28DRAFT_0817 are separated by 89 nucleotides EX28DRAFT_0817 and EX28DRAFT_0818 are separated by 170 nucleotides
EX28DRAFT_0814: EX28DRAFT_0814 - RHS repeat-associated core domain, at 816,874 to 821,232
_0814
EX28DRAFT_0815: EX28DRAFT_0815 - Macro domain, at 821,225 to 821,977
_0815
EX28DRAFT_0816: EX28DRAFT_0816 - Anaphase-promoting complex, cyclosome, subunit 3, at 822,049 to 822,468
_0816
EX28DRAFT_0817: EX28DRAFT_0817 - SMI1 / KNR4 family (SUKH-1), at 822,558 to 823,040
_0817
EX28DRAFT_0818: EX28DRAFT_0818 - hypothetical protein, at 823,211 to 823,683
_0818
Position (kb) 822
823 Strain fitness (log2 ratio) -2
-1
0
1 at 821.070 kb on - strand at 821.071 kb on + strand at 821.072 kb on - strand at 822.219 kb on + strand, within EX28DRAFT_0816 at 822.393 kb on + strand, within EX28DRAFT_0816 at 822.489 kb on - strand at 823.058 kb on - strand at 823.246 kb on + strand at 823.316 kb on - strand, within EX28DRAFT_0818 at 823.346 kb on + strand, within EX28DRAFT_0818
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 821,070 - +1.0 821,071 + -0.7 821,072 - -0.3 822,219 + EX28DRAFT_0816 0.40 -2.4 822,393 + EX28DRAFT_0816 0.82 -2.0 822,489 - +0.6 823,058 - +0.8 823,246 + -0.3 823,316 - EX28DRAFT_0818 0.22 -0.2 823,346 + EX28DRAFT_0818 0.29 +0.8
Or see this region's nucleotide sequence