Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0619 and EX28DRAFT_0620 are separated by 48 nucleotides EX28DRAFT_0620 and EX28DRAFT_0621 are separated by 5 nucleotides EX28DRAFT_0621 and EX28DRAFT_0622 are separated by 21 nucleotides
EX28DRAFT_0619: EX28DRAFT_0619 - Glycosidases, at 616,566 to 618,056
_0619
EX28DRAFT_0620: EX28DRAFT_0620 - Flagellar protein FliT, at 618,105 to 618,479
_0620
EX28DRAFT_0621: EX28DRAFT_0621 - flagellar biosynthetic protein FliS, at 618,485 to 618,889
_0621
EX28DRAFT_0622: EX28DRAFT_0622 - Flagellar capping protein, at 618,911 to 620,335
_0622
Position (kb) 618
619 Strain fitness (log2 ratio) -2
-1
0
1
2 at 617.137 kb on + strand, within EX28DRAFT_0619 at 617.224 kb on + strand, within EX28DRAFT_0619 at 617.224 kb on + strand, within EX28DRAFT_0619 at 617.224 kb on + strand, within EX28DRAFT_0619 at 617.225 kb on - strand, within EX28DRAFT_0619 at 617.225 kb on - strand, within EX28DRAFT_0619 at 617.238 kb on - strand, within EX28DRAFT_0619 at 617.271 kb on - strand, within EX28DRAFT_0619 at 617.401 kb on + strand, within EX28DRAFT_0619 at 617.402 kb on - strand, within EX28DRAFT_0619 at 617.459 kb on - strand, within EX28DRAFT_0619 at 617.459 kb on - strand, within EX28DRAFT_0619 at 617.468 kb on + strand, within EX28DRAFT_0619 at 617.469 kb on - strand, within EX28DRAFT_0619 at 617.794 kb on - strand, within EX28DRAFT_0619 at 617.794 kb on - strand, within EX28DRAFT_0619 at 617.794 kb on - strand, within EX28DRAFT_0619 at 617.878 kb on + strand, within EX28DRAFT_0619 at 617.918 kb on + strand at 618.016 kb on + strand at 618.016 kb on + strand at 618.017 kb on - strand at 618.033 kb on + strand at 618.034 kb on - strand at 618.034 kb on - strand at 618.091 kb on + strand at 618.109 kb on - strand at 618.146 kb on + strand, within EX28DRAFT_0620 at 618.171 kb on - strand, within EX28DRAFT_0620 at 618.362 kb on - strand, within EX28DRAFT_0620 at 618.473 kb on + strand at 618.548 kb on + strand, within EX28DRAFT_0621 at 618.575 kb on + strand, within EX28DRAFT_0621 at 618.617 kb on - strand, within EX28DRAFT_0621 at 618.618 kb on + strand, within EX28DRAFT_0621 at 618.780 kb on + strand, within EX28DRAFT_0621 at 618.782 kb on - strand, within EX28DRAFT_0621 at 618.845 kb on + strand, within EX28DRAFT_0621 at 618.851 kb on + strand at 618.883 kb on + strand at 618.884 kb on - strand at 618.884 kb on - strand at 618.886 kb on - strand at 618.886 kb on - strand at 618.886 kb on - strand at 618.886 kb on - strand at 618.886 kb on - strand at 618.890 kb on - strand at 618.907 kb on - strand at 618.907 kb on - strand at 618.940 kb on - strand at 618.968 kb on + strand at 618.971 kb on + strand at 619.041 kb on + strand at 619.449 kb on + strand, within EX28DRAFT_0622 at 619.450 kb on - strand, within EX28DRAFT_0622 at 619.450 kb on - strand, within EX28DRAFT_0622 at 619.450 kb on - strand, within EX28DRAFT_0622 at 619.450 kb on - strand, within EX28DRAFT_0622
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 617,137 + EX28DRAFT_0619 0.38 +0.1 617,224 + EX28DRAFT_0619 0.44 -1.1 617,224 + EX28DRAFT_0619 0.44 +0.6 617,224 + EX28DRAFT_0619 0.44 -0.1 617,225 - EX28DRAFT_0619 0.44 +0.3 617,225 - EX28DRAFT_0619 0.44 +0.5 617,238 - EX28DRAFT_0619 0.45 +0.4 617,271 - EX28DRAFT_0619 0.47 +0.3 617,401 + EX28DRAFT_0619 0.56 +0.2 617,402 - EX28DRAFT_0619 0.56 -1.2 617,459 - EX28DRAFT_0619 0.60 -0.1 617,459 - EX28DRAFT_0619 0.60 +0.5 617,468 + EX28DRAFT_0619 0.60 +0.1 617,469 - EX28DRAFT_0619 0.61 +0.8 617,794 - EX28DRAFT_0619 0.82 +0.0 617,794 - EX28DRAFT_0619 0.82 -0.1 617,794 - EX28DRAFT_0619 0.82 -0.2 617,878 + EX28DRAFT_0619 0.88 -0.0 617,918 + +1.4 618,016 + -0.5 618,016 + -0.0 618,017 - -0.3 618,033 + -2.4 618,034 - +0.4 618,034 - +1.4 618,091 + -0.2 618,109 - +0.3 618,146 + EX28DRAFT_0620 0.11 -1.6 618,171 - EX28DRAFT_0620 0.18 +2.1 618,362 - EX28DRAFT_0620 0.69 -1.6 618,473 + -0.2 618,548 + EX28DRAFT_0621 0.16 -1.5 618,575 + EX28DRAFT_0621 0.22 +0.8 618,617 - EX28DRAFT_0621 0.33 +0.3 618,618 + EX28DRAFT_0621 0.33 +0.1 618,780 + EX28DRAFT_0621 0.73 -1.4 618,782 - EX28DRAFT_0621 0.73 +0.4 618,845 + EX28DRAFT_0621 0.89 -0.2 618,851 + +0.7 618,883 + -1.2 618,884 - -0.8 618,884 - -1.5 618,886 - +0.5 618,886 - -0.8 618,886 - +0.2 618,886 - -0.2 618,886 - -0.1 618,890 - -0.4 618,907 - +1.0 618,907 - -1.9 618,940 - +1.5 618,968 + -1.4 618,971 + -2.7 619,041 + +0.4 619,449 + EX28DRAFT_0622 0.38 +1.1 619,450 - EX28DRAFT_0622 0.38 +0.4 619,450 - EX28DRAFT_0622 0.38 +0.1 619,450 - EX28DRAFT_0622 0.38 -0.4 619,450 - EX28DRAFT_0622 0.38 -0.0
Or see this region's nucleotide sequence