Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0587 and EX28DRAFT_0588 overlap by 20 nucleotides EX28DRAFT_0588 and EX28DRAFT_0589 overlap by 4 nucleotides EX28DRAFT_0589 and EX28DRAFT_0590 are separated by 164 nucleotides
EX28DRAFT_0587: EX28DRAFT_0587 - Membrane-associated lipoprotein involved in thiamine biosynthesis, at 589,715 to 590,677
_0587
EX28DRAFT_0588: EX28DRAFT_0588 - Response regulator of citrate/malate metabolism, at 590,658 to 591,377
_0588
EX28DRAFT_0589: EX28DRAFT_0589 - Signal transduction histidine kinase regulating citrate/malate metabolism, at 591,374 to 592,990
_0589
EX28DRAFT_0590: EX28DRAFT_0590 - Predicted sugar phosphate isomerase, at 593,155 to 594,018
_0590
Position (kb) 591
592
593 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 590.413 kb on + strand, within EX28DRAFT_0587 at 590.413 kb on + strand, within EX28DRAFT_0587 at 590.413 kb on + strand, within EX28DRAFT_0587 at 590.516 kb on + strand, within EX28DRAFT_0587 at 590.516 kb on + strand, within EX28DRAFT_0587 at 590.516 kb on + strand, within EX28DRAFT_0587 at 590.981 kb on - strand, within EX28DRAFT_0588 at 590.983 kb on + strand, within EX28DRAFT_0588 at 590.984 kb on - strand, within EX28DRAFT_0588 at 590.984 kb on - strand, within EX28DRAFT_0588 at 590.984 kb on - strand, within EX28DRAFT_0588 at 590.984 kb on - strand, within EX28DRAFT_0588 at 591.071 kb on - strand, within EX28DRAFT_0588 at 591.203 kb on + strand, within EX28DRAFT_0588 at 591.203 kb on + strand, within EX28DRAFT_0588 at 591.205 kb on + strand, within EX28DRAFT_0588 at 591.205 kb on + strand, within EX28DRAFT_0588 at 591.205 kb on + strand, within EX28DRAFT_0588 at 591.205 kb on + strand, within EX28DRAFT_0588 at 591.205 kb on + strand, within EX28DRAFT_0588 at 591.206 kb on - strand, within EX28DRAFT_0588 at 591.246 kb on - strand, within EX28DRAFT_0588 at 591.249 kb on - strand, within EX28DRAFT_0588 at 591.249 kb on - strand, within EX28DRAFT_0588 at 591.249 kb on - strand, within EX28DRAFT_0588 at 591.296 kb on + strand, within EX28DRAFT_0588 at 591.319 kb on + strand at 591.319 kb on + strand at 591.320 kb on - strand at 591.363 kb on + strand at 591.363 kb on + strand at 591.364 kb on - strand at 591.367 kb on - strand at 591.373 kb on - strand at 591.373 kb on - strand at 591.373 kb on - strand at 591.382 kb on + strand at 591.425 kb on - strand at 591.539 kb on - strand, within EX28DRAFT_0589 at 591.545 kb on - strand, within EX28DRAFT_0589 at 591.616 kb on - strand, within EX28DRAFT_0589 at 591.817 kb on + strand, within EX28DRAFT_0589 at 591.831 kb on - strand, within EX28DRAFT_0589 at 591.865 kb on - strand, within EX28DRAFT_0589 at 591.868 kb on + strand, within EX28DRAFT_0589 at 591.868 kb on + strand, within EX28DRAFT_0589 at 591.869 kb on - strand, within EX28DRAFT_0589 at 591.869 kb on - strand, within EX28DRAFT_0589 at 591.869 kb on - strand, within EX28DRAFT_0589 at 591.869 kb on - strand, within EX28DRAFT_0589 at 591.869 kb on - strand, within EX28DRAFT_0589 at 591.888 kb on + strand, within EX28DRAFT_0589 at 591.888 kb on + strand, within EX28DRAFT_0589 at 591.889 kb on - strand, within EX28DRAFT_0589 at 591.889 kb on - strand, within EX28DRAFT_0589 at 591.889 kb on - strand, within EX28DRAFT_0589 at 591.889 kb on - strand, within EX28DRAFT_0589 at 591.947 kb on - strand, within EX28DRAFT_0589 at 591.952 kb on + strand, within EX28DRAFT_0589 at 591.953 kb on - strand, within EX28DRAFT_0589 at 591.988 kb on - strand, within EX28DRAFT_0589 at 591.992 kb on - strand, within EX28DRAFT_0589 at 591.992 kb on - strand, within EX28DRAFT_0589 at 592.093 kb on + strand, within EX28DRAFT_0589 at 592.094 kb on - strand, within EX28DRAFT_0589 at 592.157 kb on - strand, within EX28DRAFT_0589 at 592.370 kb on - strand, within EX28DRAFT_0589 at 592.415 kb on - strand, within EX28DRAFT_0589 at 592.906 kb on + strand at 592.916 kb on + strand at 592.916 kb on + strand at 593.004 kb on + strand at 593.008 kb on + strand at 593.019 kb on + strand at 593.023 kb on + strand at 593.057 kb on - strand at 593.057 kb on - strand at 593.057 kb on - strand at 593.109 kb on + strand at 593.109 kb on + strand at 593.110 kb on - strand at 593.110 kb on - strand at 593.110 kb on - strand at 593.121 kb on + strand at 593.121 kb on + strand at 593.122 kb on - strand at 593.122 kb on - strand at 593.122 kb on - strand at 593.142 kb on - strand at 593.163 kb on + strand at 593.212 kb on - strand at 593.217 kb on + strand at 593.218 kb on - strand at 593.268 kb on + strand, within EX28DRAFT_0590 at 593.269 kb on - strand, within EX28DRAFT_0590 at 593.269 kb on - strand, within EX28DRAFT_0590 at 593.280 kb on + strand, within EX28DRAFT_0590 at 593.281 kb on - strand, within EX28DRAFT_0590 at 593.281 kb on - strand, within EX28DRAFT_0590 at 593.281 kb on - strand, within EX28DRAFT_0590 at 593.295 kb on - strand, within EX28DRAFT_0590 at 593.295 kb on - strand, within EX28DRAFT_0590 at 593.298 kb on + strand, within EX28DRAFT_0590 at 593.299 kb on - strand, within EX28DRAFT_0590 at 593.301 kb on + strand, within EX28DRAFT_0590 at 593.301 kb on + strand, within EX28DRAFT_0590 at 593.301 kb on + strand, within EX28DRAFT_0590 at 593.302 kb on - strand, within EX28DRAFT_0590 at 593.302 kb on - strand, within EX28DRAFT_0590 at 593.468 kb on - strand, within EX28DRAFT_0590 at 593.518 kb on - strand, within EX28DRAFT_0590 at 593.518 kb on - strand, within EX28DRAFT_0590 at 593.692 kb on + strand, within EX28DRAFT_0590 at 593.695 kb on - strand, within EX28DRAFT_0590 at 593.776 kb on + strand, within EX28DRAFT_0590 at 593.898 kb on + strand, within EX28DRAFT_0590 at 593.904 kb on + strand, within EX28DRAFT_0590 at 593.911 kb on - strand, within EX28DRAFT_0590 at 593.911 kb on - strand, within EX28DRAFT_0590 at 593.979 kb on + strand at 593.988 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 590,413 + EX28DRAFT_0587 0.72 +0.2 590,413 + EX28DRAFT_0587 0.72 -2.2 590,413 + EX28DRAFT_0587 0.72 -0.3 590,516 + EX28DRAFT_0587 0.83 -0.0 590,516 + EX28DRAFT_0587 0.83 +0.7 590,516 + EX28DRAFT_0587 0.83 -0.2 590,981 - EX28DRAFT_0588 0.45 +0.1 590,983 + EX28DRAFT_0588 0.45 +0.9 590,984 - EX28DRAFT_0588 0.45 -1.0 590,984 - EX28DRAFT_0588 0.45 +0.2 590,984 - EX28DRAFT_0588 0.45 -0.0 590,984 - EX28DRAFT_0588 0.45 -0.4 591,071 - EX28DRAFT_0588 0.57 +0.0 591,203 + EX28DRAFT_0588 0.76 -0.3 591,203 + EX28DRAFT_0588 0.76 -0.4 591,205 + EX28DRAFT_0588 0.76 -0.1 591,205 + EX28DRAFT_0588 0.76 -0.3 591,205 + EX28DRAFT_0588 0.76 +0.1 591,205 + EX28DRAFT_0588 0.76 +0.8 591,205 + EX28DRAFT_0588 0.76 +0.1 591,206 - EX28DRAFT_0588 0.76 -0.1 591,246 - EX28DRAFT_0588 0.82 +0.1 591,249 - EX28DRAFT_0588 0.82 +0.5 591,249 - EX28DRAFT_0588 0.82 +1.0 591,249 - EX28DRAFT_0588 0.82 +1.2 591,296 + EX28DRAFT_0588 0.89 -0.7 591,319 + +0.8 591,319 + -1.0 591,320 - +0.0 591,363 + +0.3 591,363 + -1.8 591,364 - -0.5 591,367 - -0.8 591,373 - -0.1 591,373 - -0.0 591,373 - +0.2 591,382 + -0.0 591,425 - +1.8 591,539 - EX28DRAFT_0589 0.10 +0.6 591,545 - EX28DRAFT_0589 0.11 +0.6 591,616 - EX28DRAFT_0589 0.15 +0.5 591,817 + EX28DRAFT_0589 0.27 -4.9 591,831 - EX28DRAFT_0589 0.28 -0.5 591,865 - EX28DRAFT_0589 0.30 -1.0 591,868 + EX28DRAFT_0589 0.31 +1.6 591,868 + EX28DRAFT_0589 0.31 -0.0 591,869 - EX28DRAFT_0589 0.31 +0.8 591,869 - EX28DRAFT_0589 0.31 +0.3 591,869 - EX28DRAFT_0589 0.31 +0.2 591,869 - EX28DRAFT_0589 0.31 -0.5 591,869 - EX28DRAFT_0589 0.31 -0.1 591,888 + EX28DRAFT_0589 0.32 +0.6 591,888 + EX28DRAFT_0589 0.32 +0.4 591,889 - EX28DRAFT_0589 0.32 -3.8 591,889 - EX28DRAFT_0589 0.32 +0.9 591,889 - EX28DRAFT_0589 0.32 -0.4 591,889 - EX28DRAFT_0589 0.32 -0.2 591,947 - EX28DRAFT_0589 0.35 +0.7 591,952 + EX28DRAFT_0589 0.36 +0.6 591,953 - EX28DRAFT_0589 0.36 +0.6 591,988 - EX28DRAFT_0589 0.38 -0.8 591,992 - EX28DRAFT_0589 0.38 +1.0 591,992 - EX28DRAFT_0589 0.38 +0.8 592,093 + EX28DRAFT_0589 0.44 +0.1 592,094 - EX28DRAFT_0589 0.45 -0.7 592,157 - EX28DRAFT_0589 0.48 +1.9 592,370 - EX28DRAFT_0589 0.62 -0.2 592,415 - EX28DRAFT_0589 0.64 -1.1 592,906 + +2.8 592,916 + +0.9 592,916 + +1.3 593,004 + +1.0 593,008 + +0.1 593,019 + +0.2 593,023 + -1.5 593,057 - +0.5 593,057 - -2.4 593,057 - +1.4 593,109 + +0.1 593,109 + -0.2 593,110 - -0.2 593,110 - +0.1 593,110 - -1.7 593,121 + +1.5 593,121 + -0.5 593,122 - +0.4 593,122 - +1.1 593,122 - +0.2 593,142 - +0.5 593,163 + -0.1 593,212 - +0.6 593,217 + -0.2 593,218 - +1.0 593,268 + EX28DRAFT_0590 0.13 -0.9 593,269 - EX28DRAFT_0590 0.13 -0.9 593,269 - EX28DRAFT_0590 0.13 -1.3 593,280 + EX28DRAFT_0590 0.14 -2.0 593,281 - EX28DRAFT_0590 0.15 +0.2 593,281 - EX28DRAFT_0590 0.15 +0.9 593,281 - EX28DRAFT_0590 0.15 +0.4 593,295 - EX28DRAFT_0590 0.16 -0.7 593,295 - EX28DRAFT_0590 0.16 +1.2 593,298 + EX28DRAFT_0590 0.17 -0.4 593,299 - EX28DRAFT_0590 0.17 +2.0 593,301 + EX28DRAFT_0590 0.17 -3.8 593,301 + EX28DRAFT_0590 0.17 -0.4 593,301 + EX28DRAFT_0590 0.17 -0.2 593,302 - EX28DRAFT_0590 0.17 -0.0 593,302 - EX28DRAFT_0590 0.17 -0.5 593,468 - EX28DRAFT_0590 0.36 +0.1 593,518 - EX28DRAFT_0590 0.42 -2.5 593,518 - EX28DRAFT_0590 0.42 +1.0 593,692 + EX28DRAFT_0590 0.62 +1.4 593,695 - EX28DRAFT_0590 0.62 +0.4 593,776 + EX28DRAFT_0590 0.72 +0.1 593,898 + EX28DRAFT_0590 0.86 +1.3 593,904 + EX28DRAFT_0590 0.87 +0.4 593,911 - EX28DRAFT_0590 0.88 +1.1 593,911 - EX28DRAFT_0590 0.88 +0.2 593,979 + +0.2 593,988 + +0.3
Or see this region's nucleotide sequence