Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0544 and EX28DRAFT_0545 are separated by 122 nucleotides EX28DRAFT_0545 and EX28DRAFT_0546 are separated by 210 nucleotides
EX28DRAFT_0544: EX28DRAFT_0544 - Amino acid transporters, at 537,549 to 538,907
_0544
EX28DRAFT_0545: EX28DRAFT_0545 - Exodeoxyribonuclease I subunit C (EC 3.1.11.1), at 539,030 to 540,454
_0545
EX28DRAFT_0546: EX28DRAFT_0546 - D-alanyl-D-alanine carboxypeptidase, at 540,665 to 541,831
_0546
Position (kb) 539
540
541 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4 at 538.295 kb on + strand, within EX28DRAFT_0544 at 538.308 kb on + strand, within EX28DRAFT_0544 at 538.383 kb on + strand, within EX28DRAFT_0544 at 538.383 kb on + strand, within EX28DRAFT_0544 at 538.383 kb on + strand, within EX28DRAFT_0544 at 538.384 kb on - strand, within EX28DRAFT_0544 at 538.438 kb on + strand, within EX28DRAFT_0544 at 538.485 kb on + strand, within EX28DRAFT_0544 at 538.738 kb on - strand, within EX28DRAFT_0544 at 538.845 kb on + strand at 538.846 kb on - strand at 538.891 kb on - strand at 538.943 kb on + strand at 538.944 kb on - strand at 538.944 kb on - strand at 538.944 kb on - strand at 538.944 kb on - strand at 538.944 kb on - strand at 538.947 kb on + strand at 538.948 kb on - strand at 539.019 kb on - strand at 539.019 kb on - strand at 539.019 kb on - strand at 539.036 kb on + strand at 539.036 kb on + strand at 539.036 kb on + strand at 539.049 kb on + strand at 539.130 kb on + strand at 539.135 kb on - strand at 539.286 kb on - strand, within EX28DRAFT_0545 at 539.546 kb on - strand, within EX28DRAFT_0545 at 539.551 kb on + strand, within EX28DRAFT_0545 at 539.731 kb on + strand, within EX28DRAFT_0545 at 540.462 kb on - strand at 540.489 kb on - strand at 540.489 kb on - strand at 540.493 kb on + strand at 540.597 kb on - strand at 540.597 kb on - strand at 540.660 kb on + strand at 540.660 kb on + strand at 540.660 kb on + strand at 540.661 kb on - strand at 540.661 kb on - strand at 540.841 kb on + strand, within EX28DRAFT_0546 at 540.875 kb on + strand, within EX28DRAFT_0546 at 540.876 kb on - strand, within EX28DRAFT_0546 at 541.259 kb on + strand, within EX28DRAFT_0546 at 541.259 kb on + strand, within EX28DRAFT_0546 at 541.264 kb on + strand, within EX28DRAFT_0546 at 541.264 kb on + strand, within EX28DRAFT_0546 at 541.264 kb on + strand, within EX28DRAFT_0546 at 541.265 kb on - strand, within EX28DRAFT_0546 at 541.265 kb on - strand, within EX28DRAFT_0546 at 541.265 kb on - strand, within EX28DRAFT_0546 at 541.265 kb on - strand, within EX28DRAFT_0546 at 541.268 kb on + strand, within EX28DRAFT_0546 at 541.268 kb on + strand, within EX28DRAFT_0546 at 541.268 kb on + strand, within EX28DRAFT_0546 at 541.269 kb on - strand, within EX28DRAFT_0546 at 541.271 kb on - strand, within EX28DRAFT_0546 at 541.333 kb on + strand, within EX28DRAFT_0546 at 541.369 kb on + strand, within EX28DRAFT_0546 at 541.369 kb on + strand, within EX28DRAFT_0546 at 541.370 kb on - strand, within EX28DRAFT_0546
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 538,295 + EX28DRAFT_0544 0.55 -0.0 538,308 + EX28DRAFT_0544 0.56 +0.2 538,383 + EX28DRAFT_0544 0.61 +0.1 538,383 + EX28DRAFT_0544 0.61 +0.9 538,383 + EX28DRAFT_0544 0.61 -0.3 538,384 - EX28DRAFT_0544 0.61 -0.3 538,438 + EX28DRAFT_0544 0.65 -2.9 538,485 + EX28DRAFT_0544 0.69 +0.5 538,738 - EX28DRAFT_0544 0.87 +0.3 538,845 + -0.1 538,846 - -0.4 538,891 - -0.9 538,943 + +0.0 538,944 - +0.7 538,944 - +1.2 538,944 - +0.2 538,944 - +3.8 538,944 - +1.7 538,947 + -0.8 538,948 - +0.1 539,019 - -0.4 539,019 - -0.6 539,019 - -0.8 539,036 + -0.1 539,036 + +0.5 539,036 + -0.1 539,049 + -0.3 539,130 + -1.2 539,135 - -0.5 539,286 - EX28DRAFT_0545 0.18 -0.9 539,546 - EX28DRAFT_0545 0.36 -2.3 539,551 + EX28DRAFT_0545 0.37 -1.9 539,731 + EX28DRAFT_0545 0.49 -4.9 540,462 - -0.4 540,489 - +0.3 540,489 - +0.8 540,493 + +0.3 540,597 - +1.3 540,597 - -0.2 540,660 + +0.1 540,660 + +0.8 540,660 + -0.2 540,661 - -0.1 540,661 - +0.1 540,841 + EX28DRAFT_0546 0.15 +0.1 540,875 + EX28DRAFT_0546 0.18 -0.1 540,876 - EX28DRAFT_0546 0.18 +1.2 541,259 + EX28DRAFT_0546 0.51 +0.9 541,259 + EX28DRAFT_0546 0.51 +0.1 541,264 + EX28DRAFT_0546 0.51 -0.0 541,264 + EX28DRAFT_0546 0.51 +0.2 541,264 + EX28DRAFT_0546 0.51 +0.2 541,265 - EX28DRAFT_0546 0.51 -0.3 541,265 - EX28DRAFT_0546 0.51 -0.3 541,265 - EX28DRAFT_0546 0.51 +0.5 541,265 - EX28DRAFT_0546 0.51 +0.5 541,268 + EX28DRAFT_0546 0.52 +0.4 541,268 + EX28DRAFT_0546 0.52 -0.2 541,268 + EX28DRAFT_0546 0.52 -0.7 541,269 - EX28DRAFT_0546 0.52 +0.7 541,271 - EX28DRAFT_0546 0.52 +0.0 541,333 + EX28DRAFT_0546 0.57 -2.6 541,369 + EX28DRAFT_0546 0.60 +0.7 541,369 + EX28DRAFT_0546 0.60 -2.1 541,370 - EX28DRAFT_0546 0.60 +0.1
Or see this region's nucleotide sequence