Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0539 and EX28DRAFT_0540 are separated by 5 nucleotides EX28DRAFT_0540 and EX28DRAFT_0541 are separated by 45 nucleotides EX28DRAFT_0541 and EX28DRAFT_0542 are separated by 201 nucleotides
EX28DRAFT_0539: EX28DRAFT_0539 - histidinol dehydrogenase, at 532,901 to 534,205
_0539
EX28DRAFT_0540: EX28DRAFT_0540 - ATP phosphoribosyltransferase, at 534,211 to 535,110
_0540
EX28DRAFT_0541: EX28DRAFT_0541 - RNA, at 535,156 to 535,279
_0541
EX28DRAFT_0542: EX28DRAFT_0542 - Nucleoside-diphosphate-sugar epimerases, at 535,481 to 536,305
_0542
Position (kb) 534
535
536 Strain fitness (log2 ratio) -7
-6
-5
-4
-3
-2
-1
0
1 at 533.569 kb on - strand, within EX28DRAFT_0539 at 533.569 kb on - strand, within EX28DRAFT_0539 at 533.659 kb on + strand, within EX28DRAFT_0539 at 533.659 kb on + strand, within EX28DRAFT_0539 at 533.659 kb on + strand, within EX28DRAFT_0539 at 533.660 kb on - strand, within EX28DRAFT_0539 at 533.660 kb on - strand, within EX28DRAFT_0539 at 533.660 kb on - strand, within EX28DRAFT_0539 at 533.661 kb on + strand, within EX28DRAFT_0539 at 533.662 kb on - strand, within EX28DRAFT_0539 at 533.662 kb on - strand, within EX28DRAFT_0539 at 533.817 kb on + strand, within EX28DRAFT_0539 at 533.818 kb on - strand, within EX28DRAFT_0539 at 533.818 kb on - strand, within EX28DRAFT_0539 at 533.917 kb on + strand, within EX28DRAFT_0539 at 533.918 kb on - strand, within EX28DRAFT_0539 at 534.045 kb on + strand, within EX28DRAFT_0539 at 534.389 kb on - strand, within EX28DRAFT_0540 at 534.389 kb on - strand, within EX28DRAFT_0540 at 534.389 kb on - strand, within EX28DRAFT_0540 at 534.389 kb on - strand, within EX28DRAFT_0540 at 534.428 kb on + strand, within EX28DRAFT_0540 at 534.428 kb on + strand, within EX28DRAFT_0540 at 534.429 kb on - strand, within EX28DRAFT_0540 at 534.545 kb on + strand, within EX28DRAFT_0540 at 534.732 kb on + strand, within EX28DRAFT_0540 at 534.733 kb on - strand, within EX28DRAFT_0540 at 534.733 kb on - strand, within EX28DRAFT_0540 at 534.834 kb on - strand, within EX28DRAFT_0540 at 534.939 kb on + strand, within EX28DRAFT_0540 at 535.015 kb on - strand, within EX28DRAFT_0540 at 535.081 kb on - strand at 535.081 kb on - strand at 535.084 kb on + strand at 535.084 kb on + strand at 535.085 kb on - strand at 535.085 kb on - strand at 535.135 kb on + strand at 535.257 kb on - strand, within EX28DRAFT_0541 at 535.288 kb on - strand at 535.308 kb on - strand at 535.349 kb on + strand at 535.349 kb on + strand at 535.360 kb on + strand at 535.368 kb on - strand at 535.393 kb on - strand at 535.414 kb on - strand at 535.449 kb on - strand at 535.450 kb on + strand at 535.450 kb on + strand at 535.451 kb on - strand at 535.461 kb on + strand at 535.507 kb on - strand at 535.507 kb on - strand at 535.950 kb on + strand, within EX28DRAFT_0542 at 535.951 kb on - strand, within EX28DRAFT_0542 at 535.951 kb on - strand, within EX28DRAFT_0542 at 536.076 kb on + strand, within EX28DRAFT_0542 at 536.108 kb on + strand, within EX28DRAFT_0542 at 536.109 kb on - strand, within EX28DRAFT_0542 at 536.109 kb on - strand, within EX28DRAFT_0542 at 536.109 kb on - strand, within EX28DRAFT_0542 at 536.109 kb on - strand, within EX28DRAFT_0542 at 536.109 kb on - strand, within EX28DRAFT_0542
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 533,569 - EX28DRAFT_0539 0.51 -0.7 533,569 - EX28DRAFT_0539 0.51 -5.1 533,659 + EX28DRAFT_0539 0.58 -5.0 533,659 + EX28DRAFT_0539 0.58 -3.9 533,659 + EX28DRAFT_0539 0.58 -5.0 533,660 - EX28DRAFT_0539 0.58 -0.5 533,660 - EX28DRAFT_0539 0.58 -6.4 533,660 - EX28DRAFT_0539 0.58 -4.1 533,661 + EX28DRAFT_0539 0.58 -4.0 533,662 - EX28DRAFT_0539 0.58 -6.9 533,662 - EX28DRAFT_0539 0.58 -5.0 533,817 + EX28DRAFT_0539 0.70 -3.3 533,818 - EX28DRAFT_0539 0.70 -5.4 533,818 - EX28DRAFT_0539 0.70 -3.5 533,917 + EX28DRAFT_0539 0.78 -4.3 533,918 - EX28DRAFT_0539 0.78 -3.5 534,045 + EX28DRAFT_0539 0.88 -4.4 534,389 - EX28DRAFT_0540 0.20 -4.5 534,389 - EX28DRAFT_0540 0.20 -4.0 534,389 - EX28DRAFT_0540 0.20 -4.8 534,389 - EX28DRAFT_0540 0.20 -2.3 534,428 + EX28DRAFT_0540 0.24 -2.3 534,428 + EX28DRAFT_0540 0.24 -2.3 534,429 - EX28DRAFT_0540 0.24 -0.9 534,545 + EX28DRAFT_0540 0.37 -3.6 534,732 + EX28DRAFT_0540 0.58 -5.7 534,733 - EX28DRAFT_0540 0.58 -5.3 534,733 - EX28DRAFT_0540 0.58 -2.6 534,834 - EX28DRAFT_0540 0.69 -5.8 534,939 + EX28DRAFT_0540 0.81 -5.4 535,015 - EX28DRAFT_0540 0.89 -3.4 535,081 - -3.8 535,081 - -0.9 535,084 + -5.9 535,084 + -4.8 535,085 - -3.9 535,085 - -2.6 535,135 + -5.3 535,257 - EX28DRAFT_0541 0.81 +1.2 535,288 - -3.3 535,308 - -2.1 535,349 + -1.3 535,349 + -3.9 535,360 + -3.2 535,368 - +0.3 535,393 - +0.1 535,414 - +1.0 535,449 - -1.9 535,450 + +0.2 535,450 + -0.6 535,451 - -1.3 535,461 + +0.3 535,507 - -0.4 535,507 - -0.8 535,950 + EX28DRAFT_0542 0.57 -1.5 535,951 - EX28DRAFT_0542 0.57 -0.2 535,951 - EX28DRAFT_0542 0.57 +0.3 536,076 + EX28DRAFT_0542 0.72 -1.3 536,108 + EX28DRAFT_0542 0.76 +1.2 536,109 - EX28DRAFT_0542 0.76 -0.0 536,109 - EX28DRAFT_0542 0.76 +0.2 536,109 - EX28DRAFT_0542 0.76 -0.1 536,109 - EX28DRAFT_0542 0.76 -0.6 536,109 - EX28DRAFT_0542 0.76 +0.3
Or see this region's nucleotide sequence