Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0487 and EX28DRAFT_0488 are separated by 343 nucleotides EX28DRAFT_0488 and EX28DRAFT_0489 are separated by 91 nucleotides EX28DRAFT_0489 and EX28DRAFT_0490 are separated by 22 nucleotides
EX28DRAFT_0487: EX28DRAFT_0487 - PAS domain S-box/diguanylate cyclase (GGDEF) domain, at 475,275 to 478,604
_0487
EX28DRAFT_0488: EX28DRAFT_0488 - uridine kinase (EC 2.7.1.48), at 478,948 to 479,589
_0488
EX28DRAFT_0489: EX28DRAFT_0489 - deoxycytidine triphosphate deaminase, at 479,681 to 480,262
_0489
EX28DRAFT_0490: EX28DRAFT_0490 - Uncharacterized protein involved in outer membrane biogenesis, at 480,285 to 482,135
_0490
Position (kb) 478
479
480 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 478.064 kb on - strand, within EX28DRAFT_0487 at 478.077 kb on + strand, within EX28DRAFT_0487 at 478.078 kb on - strand, within EX28DRAFT_0487 at 478.078 kb on - strand, within EX28DRAFT_0487 at 478.142 kb on + strand, within EX28DRAFT_0487 at 478.261 kb on + strand, within EX28DRAFT_0487 at 478.261 kb on + strand, within EX28DRAFT_0487 at 478.261 kb on + strand, within EX28DRAFT_0487 at 478.327 kb on + strand at 478.328 kb on - strand at 478.328 kb on - strand at 478.377 kb on - strand at 478.512 kb on + strand at 478.513 kb on - strand at 478.513 kb on - strand at 478.518 kb on - strand at 478.524 kb on - strand at 478.557 kb on + strand at 478.574 kb on + strand at 478.592 kb on - strand at 478.592 kb on - strand at 478.664 kb on + strand at 478.674 kb on + strand at 478.674 kb on + strand at 478.680 kb on - strand at 478.739 kb on + strand at 478.739 kb on + strand at 478.740 kb on - strand at 478.881 kb on - strand at 478.881 kb on - strand at 478.957 kb on - strand at 478.957 kb on - strand at 478.957 kb on - strand at 478.977 kb on + strand at 479.026 kb on - strand, within EX28DRAFT_0488 at 479.054 kb on + strand, within EX28DRAFT_0488 at 479.055 kb on - strand, within EX28DRAFT_0488 at 479.092 kb on + strand, within EX28DRAFT_0488 at 479.092 kb on + strand, within EX28DRAFT_0488 at 479.143 kb on + strand, within EX28DRAFT_0488 at 479.143 kb on + strand, within EX28DRAFT_0488 at 479.144 kb on - strand, within EX28DRAFT_0488 at 479.144 kb on - strand, within EX28DRAFT_0488 at 479.190 kb on - strand, within EX28DRAFT_0488 at 479.231 kb on - strand, within EX28DRAFT_0488 at 479.237 kb on - strand, within EX28DRAFT_0488 at 479.408 kb on + strand, within EX28DRAFT_0488 at 479.409 kb on - strand, within EX28DRAFT_0488 at 479.449 kb on + strand, within EX28DRAFT_0488 at 479.504 kb on - strand, within EX28DRAFT_0488 at 479.533 kb on - strand at 479.548 kb on - strand at 479.548 kb on - strand at 479.548 kb on - strand at 479.548 kb on - strand at 479.559 kb on - strand at 479.559 kb on - strand at 480.384 kb on + strand at 480.384 kb on + strand at 480.385 kb on - strand at 480.385 kb on - strand at 480.485 kb on + strand, within EX28DRAFT_0490
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 478,064 - EX28DRAFT_0487 0.84 -0.3 478,077 + EX28DRAFT_0487 0.84 +0.3 478,078 - EX28DRAFT_0487 0.84 -0.4 478,078 - EX28DRAFT_0487 0.84 +0.5 478,142 + EX28DRAFT_0487 0.86 +0.0 478,261 + EX28DRAFT_0487 0.90 -0.7 478,261 + EX28DRAFT_0487 0.90 -0.5 478,261 + EX28DRAFT_0487 0.90 +0.4 478,327 + +1.3 478,328 - +0.0 478,328 - +0.1 478,377 - +0.3 478,512 + +0.3 478,513 - +0.6 478,513 - +0.6 478,518 - -0.7 478,524 - +0.7 478,557 + -0.5 478,574 + +0.0 478,592 - -0.3 478,592 - -0.3 478,664 + -0.3 478,674 + +0.5 478,674 + -0.1 478,680 - -0.9 478,739 + -0.5 478,739 + +0.5 478,740 - -0.1 478,881 - +0.4 478,881 - -0.4 478,957 - -0.3 478,957 - +0.1 478,957 - -0.6 478,977 + +0.2 479,026 - EX28DRAFT_0488 0.12 -0.3 479,054 + EX28DRAFT_0488 0.17 +0.4 479,055 - EX28DRAFT_0488 0.17 -1.3 479,092 + EX28DRAFT_0488 0.22 -0.7 479,092 + EX28DRAFT_0488 0.22 -4.9 479,143 + EX28DRAFT_0488 0.30 -2.0 479,143 + EX28DRAFT_0488 0.30 +0.4 479,144 - EX28DRAFT_0488 0.31 -0.9 479,144 - EX28DRAFT_0488 0.31 +1.0 479,190 - EX28DRAFT_0488 0.38 +0.2 479,231 - EX28DRAFT_0488 0.44 -1.1 479,237 - EX28DRAFT_0488 0.45 +0.4 479,408 + EX28DRAFT_0488 0.72 -0.1 479,409 - EX28DRAFT_0488 0.72 -0.5 479,449 + EX28DRAFT_0488 0.78 -0.9 479,504 - EX28DRAFT_0488 0.87 -3.4 479,533 - -0.4 479,548 - +1.3 479,548 - -0.3 479,548 - -0.0 479,548 - -0.3 479,559 - -0.4 479,559 - -0.0 480,384 + +0.5 480,384 + -1.1 480,385 - +0.4 480,385 - -0.5 480,485 + EX28DRAFT_0490 0.11 +0.9
Or see this region's nucleotide sequence