Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0384 and EX28DRAFT_0385 are separated by 74 nucleotides EX28DRAFT_0385 and EX28DRAFT_0386 are separated by 127 nucleotides
EX28DRAFT_0384: EX28DRAFT_0384 - ABC-type oligopeptide transport system, periplasmic component, at 356,216 to 358,021
_0384
EX28DRAFT_0385: EX28DRAFT_0385 - FOG: EAL domain, at 358,096 to 359,652
_0385
EX28DRAFT_0386: EX28DRAFT_0386 - Cell wall-associated hydrolases (invasion-associated proteins), at 359,780 to 360,349
_0386
Position (kb) 358
359
360 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 357.144 kb on + strand, within EX28DRAFT_0384 at 357.144 kb on + strand, within EX28DRAFT_0384 at 357.145 kb on - strand, within EX28DRAFT_0384 at 357.209 kb on + strand, within EX28DRAFT_0384 at 357.262 kb on - strand, within EX28DRAFT_0384 at 357.262 kb on - strand, within EX28DRAFT_0384 at 357.264 kb on + strand, within EX28DRAFT_0384 at 357.345 kb on + strand, within EX28DRAFT_0384 at 357.354 kb on + strand, within EX28DRAFT_0384 at 357.355 kb on - strand, within EX28DRAFT_0384 at 357.430 kb on + strand, within EX28DRAFT_0384 at 357.431 kb on - strand, within EX28DRAFT_0384 at 357.484 kb on + strand, within EX28DRAFT_0384 at 357.484 kb on + strand, within EX28DRAFT_0384 at 357.729 kb on + strand, within EX28DRAFT_0384 at 357.915 kb on + strand at 358.016 kb on - strand at 358.016 kb on - strand at 358.070 kb on + strand at 358.071 kb on - strand at 358.071 kb on - strand at 358.071 kb on - strand at 358.071 kb on - strand at 358.251 kb on + strand at 358.324 kb on - strand, within EX28DRAFT_0385 at 358.349 kb on + strand, within EX28DRAFT_0385 at 358.349 kb on + strand, within EX28DRAFT_0385 at 358.489 kb on - strand, within EX28DRAFT_0385 at 358.545 kb on + strand, within EX28DRAFT_0385 at 358.616 kb on - strand, within EX28DRAFT_0385 at 358.821 kb on - strand, within EX28DRAFT_0385 at 358.847 kb on - strand, within EX28DRAFT_0385 at 358.876 kb on - strand, within EX28DRAFT_0385 at 358.885 kb on - strand, within EX28DRAFT_0385 at 358.885 kb on - strand, within EX28DRAFT_0385 at 358.886 kb on + strand, within EX28DRAFT_0385 at 358.887 kb on - strand, within EX28DRAFT_0385 at 358.887 kb on - strand, within EX28DRAFT_0385 at 358.889 kb on + strand, within EX28DRAFT_0385 at 358.889 kb on + strand, within EX28DRAFT_0385 at 358.889 kb on + strand, within EX28DRAFT_0385 at 358.890 kb on - strand, within EX28DRAFT_0385 at 358.890 kb on - strand, within EX28DRAFT_0385 at 358.890 kb on - strand, within EX28DRAFT_0385 at 358.982 kb on - strand, within EX28DRAFT_0385 at 359.088 kb on + strand, within EX28DRAFT_0385 at 359.088 kb on + strand, within EX28DRAFT_0385 at 359.314 kb on - strand, within EX28DRAFT_0385 at 359.403 kb on + strand, within EX28DRAFT_0385 at 359.495 kb on + strand, within EX28DRAFT_0385 at 359.495 kb on + strand, within EX28DRAFT_0385 at 359.495 kb on + strand, within EX28DRAFT_0385 at 359.495 kb on + strand, within EX28DRAFT_0385 at 359.495 kb on + strand, within EX28DRAFT_0385 at 359.495 kb on + strand, within EX28DRAFT_0385 at 359.547 kb on - strand at 359.617 kb on + strand at 359.652 kb on + strand at 359.652 kb on + strand at 359.685 kb on + strand at 359.685 kb on + strand at 359.779 kb on - strand at 359.779 kb on - strand at 359.781 kb on + strand at 359.782 kb on - strand at 359.796 kb on - strand at 359.880 kb on - strand, within EX28DRAFT_0386 at 359.880 kb on - strand, within EX28DRAFT_0386 at 359.945 kb on + strand, within EX28DRAFT_0386 at 359.946 kb on - strand, within EX28DRAFT_0386 at 360.003 kb on + strand, within EX28DRAFT_0386 at 360.023 kb on + strand, within EX28DRAFT_0386 at 360.047 kb on - strand, within EX28DRAFT_0386 at 360.053 kb on + strand, within EX28DRAFT_0386 at 360.074 kb on - strand, within EX28DRAFT_0386 at 360.127 kb on - strand, within EX28DRAFT_0386 at 360.137 kb on - strand, within EX28DRAFT_0386 at 360.160 kb on + strand, within EX28DRAFT_0386 at 360.161 kb on - strand, within EX28DRAFT_0386 at 360.165 kb on - strand, within EX28DRAFT_0386 at 360.169 kb on + strand, within EX28DRAFT_0386 at 360.266 kb on - strand, within EX28DRAFT_0386 at 360.268 kb on + strand, within EX28DRAFT_0386 at 360.269 kb on - strand, within EX28DRAFT_0386 at 360.269 kb on - strand, within EX28DRAFT_0386 at 360.269 kb on - strand, within EX28DRAFT_0386 at 360.269 kb on - strand, within EX28DRAFT_0386 at 360.269 kb on - strand, within EX28DRAFT_0386 at 360.319 kb on + strand at 360.320 kb on - strand at 360.322 kb on - strand at 360.385 kb on + strand at 360.386 kb on - strand at 360.393 kb on + strand at 360.393 kb on + strand at 360.421 kb on + strand at 360.499 kb on - strand at 360.499 kb on - strand at 360.596 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 357,144 + EX28DRAFT_0384 0.51 -0.1 357,144 + EX28DRAFT_0384 0.51 -0.2 357,145 - EX28DRAFT_0384 0.51 -1.0 357,209 + EX28DRAFT_0384 0.55 -3.2 357,262 - EX28DRAFT_0384 0.58 +1.0 357,262 - EX28DRAFT_0384 0.58 +0.7 357,264 + EX28DRAFT_0384 0.58 -1.2 357,345 + EX28DRAFT_0384 0.63 +0.7 357,354 + EX28DRAFT_0384 0.63 -0.4 357,355 - EX28DRAFT_0384 0.63 -0.4 357,430 + EX28DRAFT_0384 0.67 +3.3 357,431 - EX28DRAFT_0384 0.67 +0.5 357,484 + EX28DRAFT_0384 0.70 +0.3 357,484 + EX28DRAFT_0384 0.70 -0.5 357,729 + EX28DRAFT_0384 0.84 +0.0 357,915 + +0.0 358,016 - +0.0 358,016 - +0.1 358,070 + +0.0 358,071 - +0.8 358,071 - +0.5 358,071 - +0.6 358,071 - +0.8 358,251 + +1.0 358,324 - EX28DRAFT_0385 0.15 -0.5 358,349 + EX28DRAFT_0385 0.16 -0.2 358,349 + EX28DRAFT_0385 0.16 -0.2 358,489 - EX28DRAFT_0385 0.25 -1.0 358,545 + EX28DRAFT_0385 0.29 +0.3 358,616 - EX28DRAFT_0385 0.33 -1.1 358,821 - EX28DRAFT_0385 0.47 +1.8 358,847 - EX28DRAFT_0385 0.48 +0.3 358,876 - EX28DRAFT_0385 0.50 -0.1 358,885 - EX28DRAFT_0385 0.51 -0.1 358,885 - EX28DRAFT_0385 0.51 -1.0 358,886 + EX28DRAFT_0385 0.51 -1.8 358,887 - EX28DRAFT_0385 0.51 -0.6 358,887 - EX28DRAFT_0385 0.51 -0.1 358,889 + EX28DRAFT_0385 0.51 +0.6 358,889 + EX28DRAFT_0385 0.51 +0.2 358,889 + EX28DRAFT_0385 0.51 +1.5 358,890 - EX28DRAFT_0385 0.51 +0.9 358,890 - EX28DRAFT_0385 0.51 +0.4 358,890 - EX28DRAFT_0385 0.51 +0.4 358,982 - EX28DRAFT_0385 0.57 +0.1 359,088 + EX28DRAFT_0385 0.64 -0.4 359,088 + EX28DRAFT_0385 0.64 -0.0 359,314 - EX28DRAFT_0385 0.78 +1.7 359,403 + EX28DRAFT_0385 0.84 -0.5 359,495 + EX28DRAFT_0385 0.90 -0.5 359,495 + EX28DRAFT_0385 0.90 -0.2 359,495 + EX28DRAFT_0385 0.90 -1.1 359,495 + EX28DRAFT_0385 0.90 -0.2 359,495 + EX28DRAFT_0385 0.90 -0.0 359,495 + EX28DRAFT_0385 0.90 -0.2 359,547 - +1.2 359,617 + -0.3 359,652 + -0.5 359,652 + +0.9 359,685 + +2.9 359,685 + -0.7 359,779 - -0.1 359,779 - +0.4 359,781 + -2.7 359,782 - +0.8 359,796 - +0.0 359,880 - EX28DRAFT_0386 0.18 +0.7 359,880 - EX28DRAFT_0386 0.18 +0.8 359,945 + EX28DRAFT_0386 0.29 -2.9 359,946 - EX28DRAFT_0386 0.29 -0.3 360,003 + EX28DRAFT_0386 0.39 +0.0 360,023 + EX28DRAFT_0386 0.43 -0.4 360,047 - EX28DRAFT_0386 0.47 +0.4 360,053 + EX28DRAFT_0386 0.48 -0.1 360,074 - EX28DRAFT_0386 0.52 -0.2 360,127 - EX28DRAFT_0386 0.61 -1.9 360,137 - EX28DRAFT_0386 0.63 +0.4 360,160 + EX28DRAFT_0386 0.67 +0.2 360,161 - EX28DRAFT_0386 0.67 +0.5 360,165 - EX28DRAFT_0386 0.68 -0.8 360,169 + EX28DRAFT_0386 0.68 -2.2 360,266 - EX28DRAFT_0386 0.85 +1.1 360,268 + EX28DRAFT_0386 0.86 +2.9 360,269 - EX28DRAFT_0386 0.86 +0.4 360,269 - EX28DRAFT_0386 0.86 +0.3 360,269 - EX28DRAFT_0386 0.86 -0.0 360,269 - EX28DRAFT_0386 0.86 +0.6 360,269 - EX28DRAFT_0386 0.86 +0.3 360,319 + -0.4 360,320 - +0.2 360,322 - +0.2 360,385 + +2.0 360,386 - +0.4 360,393 + +1.2 360,393 + +1.1 360,421 + +0.4 360,499 - -0.1 360,499 - +0.3 360,596 + +0.7
Or see this region's nucleotide sequence