Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0272 and EX28DRAFT_0273 are separated by 46 nucleotides EX28DRAFT_0273 and EX28DRAFT_0274 are separated by 145 nucleotides EX28DRAFT_0274 and EX28DRAFT_0275 are separated by 60 nucleotides
EX28DRAFT_0272: EX28DRAFT_0272 - phosphohistidine phosphatase, SixA, at 234,977 to 235,447
_0272
EX28DRAFT_0273: EX28DRAFT_0273 - Uncharacterized protein conserved in bacteria, at 235,494 to 236,045
_0273
EX28DRAFT_0274: EX28DRAFT_0274 - protein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific, at 236,191 to 237,123
_0274
EX28DRAFT_0275: EX28DRAFT_0275 - chorismate synthase (EC 4.2.3.5), at 237,184 to 238,269
_0275
Position (kb) 236
237
238 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 235.233 kb on - strand, within EX28DRAFT_0272 at 235.562 kb on - strand, within EX28DRAFT_0273 at 235.631 kb on + strand, within EX28DRAFT_0273 at 235.637 kb on + strand, within EX28DRAFT_0273 at 235.821 kb on + strand, within EX28DRAFT_0273 at 235.822 kb on - strand, within EX28DRAFT_0273 at 235.825 kb on + strand, within EX28DRAFT_0273 at 235.825 kb on + strand, within EX28DRAFT_0273 at 235.825 kb on + strand, within EX28DRAFT_0273 at 235.826 kb on - strand, within EX28DRAFT_0273 at 235.826 kb on - strand, within EX28DRAFT_0273 at 235.920 kb on + strand, within EX28DRAFT_0273 at 235.920 kb on + strand, within EX28DRAFT_0273 at 236.152 kb on - strand at 236.197 kb on - strand at 236.201 kb on + strand at 236.233 kb on - strand at 236.237 kb on + strand at 236.238 kb on - strand at 236.284 kb on - strand at 236.504 kb on - strand, within EX28DRAFT_0274 at 236.504 kb on - strand, within EX28DRAFT_0274 at 236.644 kb on + strand, within EX28DRAFT_0274 at 236.791 kb on + strand, within EX28DRAFT_0274 at 236.942 kb on - strand, within EX28DRAFT_0274 at 236.990 kb on + strand, within EX28DRAFT_0274 at 236.990 kb on + strand, within EX28DRAFT_0274 at 236.990 kb on + strand, within EX28DRAFT_0274 at 236.991 kb on - strand, within EX28DRAFT_0274 at 236.991 kb on - strand, within EX28DRAFT_0274 at 237.063 kb on + strand at 237.113 kb on - strand at 237.113 kb on - strand at 237.121 kb on + strand at 237.848 kb on + strand, within EX28DRAFT_0275
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 235,233 - EX28DRAFT_0272 0.54 -5.3 235,562 - EX28DRAFT_0273 0.12 -2.4 235,631 + EX28DRAFT_0273 0.25 +1.2 235,637 + EX28DRAFT_0273 0.26 +0.2 235,821 + EX28DRAFT_0273 0.59 +0.4 235,822 - EX28DRAFT_0273 0.59 -0.2 235,825 + EX28DRAFT_0273 0.60 +0.5 235,825 + EX28DRAFT_0273 0.60 -1.7 235,825 + EX28DRAFT_0273 0.60 +0.5 235,826 - EX28DRAFT_0273 0.60 +0.1 235,826 - EX28DRAFT_0273 0.60 -2.1 235,920 + EX28DRAFT_0273 0.77 -0.3 235,920 + EX28DRAFT_0273 0.77 -0.2 236,152 - -3.6 236,197 - -1.0 236,201 + -0.6 236,233 - -0.8 236,237 + -0.8 236,238 - -0.7 236,284 - -0.3 236,504 - EX28DRAFT_0274 0.34 -3.3 236,504 - EX28DRAFT_0274 0.34 -1.2 236,644 + EX28DRAFT_0274 0.49 +1.1 236,791 + EX28DRAFT_0274 0.64 -0.5 236,942 - EX28DRAFT_0274 0.80 -1.1 236,990 + EX28DRAFT_0274 0.86 +0.6 236,990 + EX28DRAFT_0274 0.86 -1.2 236,990 + EX28DRAFT_0274 0.86 -0.3 236,991 - EX28DRAFT_0274 0.86 -1.4 236,991 - EX28DRAFT_0274 0.86 -4.8 237,063 + +0.2 237,113 - -5.1 237,113 - -4.6 237,121 + +0.1 237,848 + EX28DRAFT_0275 0.61 -4.8
Or see this region's nucleotide sequence