Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0217 and EX28DRAFT_0218 are separated by 1 nucleotides EX28DRAFT_0218 and EX28DRAFT_0219 overlap by 4 nucleotides EX28DRAFT_0219 and EX28DRAFT_0220 are separated by 80 nucleotides
EX28DRAFT_0217: EX28DRAFT_0217 - DNA ligase, NAD-dependent, at 171,333 to 173,348
_0217
EX28DRAFT_0218: EX28DRAFT_0218 - Uncharacterized protein conserved in bacteria, at 173,350 to 173,565
_0218
EX28DRAFT_0219: EX28DRAFT_0219 - Predicted Na+-dependent transporter, at 173,562 to 174,557
_0219
EX28DRAFT_0220: EX28DRAFT_0220 - Transcriptional regulator, at 174,638 to 175,582
_0220
Position (kb) 173
174
175 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 173.346 kb on + strand at 173.383 kb on - strand, within EX28DRAFT_0218 at 173.404 kb on + strand, within EX28DRAFT_0218 at 173.405 kb on - strand, within EX28DRAFT_0218 at 173.440 kb on + strand, within EX28DRAFT_0218 at 173.510 kb on + strand, within EX28DRAFT_0218 at 173.564 kb on - strand at 173.618 kb on - strand at 173.865 kb on - strand, within EX28DRAFT_0219 at 173.891 kb on + strand, within EX28DRAFT_0219 at 174.080 kb on + strand, within EX28DRAFT_0219 at 174.203 kb on + strand, within EX28DRAFT_0219 at 174.204 kb on - strand, within EX28DRAFT_0219 at 174.204 kb on - strand, within EX28DRAFT_0219 at 174.204 kb on - strand, within EX28DRAFT_0219 at 174.247 kb on + strand, within EX28DRAFT_0219 at 174.383 kb on + strand, within EX28DRAFT_0219 at 174.416 kb on + strand, within EX28DRAFT_0219 at 174.501 kb on + strand at 174.550 kb on - strand at 174.550 kb on - strand at 174.558 kb on - strand at 174.584 kb on - strand at 174.660 kb on + strand at 174.660 kb on + strand at 174.673 kb on + strand at 174.673 kb on + strand at 174.980 kb on - strand, within EX28DRAFT_0220 at 175.290 kb on + strand, within EX28DRAFT_0220 at 175.344 kb on + strand, within EX28DRAFT_0220 at 175.498 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 173,346 + +0.8 173,383 - EX28DRAFT_0218 0.15 +0.7 173,404 + EX28DRAFT_0218 0.25 +0.3 173,405 - EX28DRAFT_0218 0.25 +0.6 173,440 + EX28DRAFT_0218 0.42 -1.7 173,510 + EX28DRAFT_0218 0.74 +0.1 173,564 - -1.1 173,618 - +1.3 173,865 - EX28DRAFT_0219 0.30 +0.5 173,891 + EX28DRAFT_0219 0.33 -1.4 174,080 + EX28DRAFT_0219 0.52 +2.2 174,203 + EX28DRAFT_0219 0.64 -3.8 174,204 - EX28DRAFT_0219 0.64 -2.7 174,204 - EX28DRAFT_0219 0.64 -0.5 174,204 - EX28DRAFT_0219 0.64 -0.1 174,247 + EX28DRAFT_0219 0.69 +0.2 174,383 + EX28DRAFT_0219 0.82 -2.7 174,416 + EX28DRAFT_0219 0.86 -2.1 174,501 + -3.3 174,550 - -3.5 174,550 - -0.7 174,558 - -0.1 174,584 - +0.4 174,660 + -0.3 174,660 + -2.4 174,673 + -1.1 174,673 + +0.0 174,980 - EX28DRAFT_0220 0.36 +0.1 175,290 + EX28DRAFT_0220 0.69 +0.3 175,344 + EX28DRAFT_0220 0.75 -0.0 175,498 + -1.9
Or see this region's nucleotide sequence