Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0212 and EX28DRAFT_0213 are separated by 44 nucleotides EX28DRAFT_0213 and EX28DRAFT_0214 are separated by 317 nucleotides EX28DRAFT_0214 and EX28DRAFT_0215 are separated by 163 nucleotides
EX28DRAFT_0212: EX28DRAFT_0212 - phosphoenolpyruvate-protein phosphotransferase, at 165,818 to 167,545
_0212
EX28DRAFT_0213: EX28DRAFT_0213 - Phosphotransferase System HPr (HPr) Family, at 167,590 to 167,847
_0213
EX28DRAFT_0214: EX28DRAFT_0214 - cysteine synthase A, at 168,165 to 169,136
_0214
EX28DRAFT_0215: EX28DRAFT_0215 - Uncharacterized protein involved in cysteine biosynthesis, at 169,300 to 170,061
_0215
Position (kb) 168
169
170 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 167.864 kb on - strand at 168.573 kb on - strand, within EX28DRAFT_0214 at 168.575 kb on - strand, within EX28DRAFT_0214 at 168.621 kb on - strand, within EX28DRAFT_0214 at 168.621 kb on - strand, within EX28DRAFT_0214 at 168.724 kb on + strand, within EX28DRAFT_0214 at 168.834 kb on - strand, within EX28DRAFT_0214 at 169.094 kb on + strand at 169.095 kb on - strand at 169.154 kb on - strand at 169.154 kb on - strand at 169.179 kb on + strand at 169.180 kb on - strand at 169.204 kb on + strand at 169.204 kb on + strand at 169.205 kb on - strand at 169.218 kb on - strand at 169.218 kb on - strand at 169.221 kb on + strand at 169.221 kb on + strand at 169.221 kb on + strand at 169.221 kb on + strand at 169.221 kb on + strand at 169.222 kb on - strand at 169.222 kb on - strand at 169.224 kb on - strand at 169.239 kb on - strand at 169.287 kb on - strand at 169.287 kb on - strand at 169.592 kb on + strand, within EX28DRAFT_0215 at 169.594 kb on + strand, within EX28DRAFT_0215 at 169.841 kb on + strand, within EX28DRAFT_0215 at 169.842 kb on - strand, within EX28DRAFT_0215 at 169.871 kb on + strand, within EX28DRAFT_0215 at 169.941 kb on + strand, within EX28DRAFT_0215 at 169.941 kb on + strand, within EX28DRAFT_0215 at 169.954 kb on + strand, within EX28DRAFT_0215 at 170.072 kb on + strand at 170.107 kb on + strand at 170.108 kb on - strand at 170.109 kb on + strand at 170.109 kb on + strand at 170.109 kb on + strand at 170.109 kb on + strand at 170.110 kb on - strand at 170.110 kb on - strand at 170.121 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 167,864 - -1.7 168,573 - EX28DRAFT_0214 0.42 -3.4 168,575 - EX28DRAFT_0214 0.42 -4.8 168,621 - EX28DRAFT_0214 0.47 -4.1 168,621 - EX28DRAFT_0214 0.47 -3.7 168,724 + EX28DRAFT_0214 0.58 -5.6 168,834 - EX28DRAFT_0214 0.69 -2.4 169,094 + -2.3 169,095 - -1.1 169,154 - -2.7 169,154 - -0.8 169,179 + -0.1 169,180 - -1.5 169,204 + -4.0 169,204 + -4.2 169,205 - -2.4 169,218 - -1.7 169,218 - -1.2 169,221 + -3.2 169,221 + -2.9 169,221 + -5.3 169,221 + -1.8 169,221 + -5.0 169,222 - -0.4 169,222 - -3.1 169,224 - -3.7 169,239 - -0.3 169,287 - -0.3 169,287 - +0.0 169,592 + EX28DRAFT_0215 0.38 -0.2 169,594 + EX28DRAFT_0215 0.39 +0.5 169,841 + EX28DRAFT_0215 0.71 +3.0 169,842 - EX28DRAFT_0215 0.71 +0.3 169,871 + EX28DRAFT_0215 0.75 +0.6 169,941 + EX28DRAFT_0215 0.84 +0.1 169,941 + EX28DRAFT_0215 0.84 +0.3 169,954 + EX28DRAFT_0215 0.86 -0.4 170,072 + +0.6 170,107 + +0.4 170,108 - +1.9 170,109 + -0.1 170,109 + +0.3 170,109 + -0.2 170,109 + -0.5 170,110 - -0.4 170,110 - -0.5 170,121 - +0.0
Or see this region's nucleotide sequence