Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0205 and EX28DRAFT_0206 overlap by 1 nucleotides EX28DRAFT_0206 and EX28DRAFT_0207 overlap by 1 nucleotides EX28DRAFT_0207 and EX28DRAFT_0208 overlap by 11 nucleotides
EX28DRAFT_0205: EX28DRAFT_0205 - sulfate/thiosulfate-binding protein, at 159,546 to 160,559
_0205
EX28DRAFT_0206: EX28DRAFT_0206 - sulfate ABC transporter, permease protein CysT, at 160,559 to 161,392
_0206
EX28DRAFT_0207: EX28DRAFT_0207 - sulfate ABC transporter, permease protein CysW, at 161,392 to 162,267
_0207
EX28DRAFT_0208: EX28DRAFT_0208 - sulfate ABC transporter, ATP-binding protein, at 162,257 to 163,351
_0208
Position (kb) 161
162
163 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 160.393 kb on - strand, within EX28DRAFT_0205 at 160.393 kb on - strand, within EX28DRAFT_0205 at 160.417 kb on + strand, within EX28DRAFT_0205 at 160.418 kb on - strand, within EX28DRAFT_0205 at 160.418 kb on - strand, within EX28DRAFT_0205 at 160.436 kb on + strand, within EX28DRAFT_0205 at 160.437 kb on - strand, within EX28DRAFT_0205 at 160.528 kb on + strand at 160.553 kb on + strand at 160.553 kb on + strand at 160.553 kb on + strand at 160.554 kb on - strand at 160.557 kb on + strand at 160.557 kb on + strand at 160.558 kb on - strand at 160.558 kb on - strand at 160.603 kb on - strand at 160.738 kb on + strand, within EX28DRAFT_0206 at 160.738 kb on + strand, within EX28DRAFT_0206 at 160.739 kb on - strand, within EX28DRAFT_0206 at 160.970 kb on + strand, within EX28DRAFT_0206 at 160.970 kb on + strand, within EX28DRAFT_0206 at 160.970 kb on + strand, within EX28DRAFT_0206 at 160.971 kb on - strand, within EX28DRAFT_0206 at 161.382 kb on + strand at 161.382 kb on + strand at 161.383 kb on - strand at 161.383 kb on - strand at 161.404 kb on - strand at 161.509 kb on + strand, within EX28DRAFT_0207 at 161.509 kb on + strand, within EX28DRAFT_0207 at 161.510 kb on - strand, within EX28DRAFT_0207 at 161.510 kb on - strand, within EX28DRAFT_0207 at 161.511 kb on + strand, within EX28DRAFT_0207 at 161.512 kb on - strand, within EX28DRAFT_0207 at 161.753 kb on - strand, within EX28DRAFT_0207 at 161.764 kb on + strand, within EX28DRAFT_0207 at 161.765 kb on - strand, within EX28DRAFT_0207 at 161.765 kb on - strand, within EX28DRAFT_0207 at 161.805 kb on + strand, within EX28DRAFT_0207 at 162.004 kb on + strand, within EX28DRAFT_0207 at 162.128 kb on - strand, within EX28DRAFT_0207 at 162.278 kb on - strand at 162.280 kb on + strand at 162.280 kb on + strand at 162.281 kb on - strand at 162.316 kb on + strand at 162.478 kb on + strand, within EX28DRAFT_0208 at 162.493 kb on - strand, within EX28DRAFT_0208 at 162.503 kb on + strand, within EX28DRAFT_0208 at 162.992 kb on - strand, within EX28DRAFT_0208 at 163.169 kb on + strand, within EX28DRAFT_0208 at 163.169 kb on + strand, within EX28DRAFT_0208 at 163.186 kb on - strand, within EX28DRAFT_0208
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 160,393 - EX28DRAFT_0205 0.84 -0.3 160,393 - EX28DRAFT_0205 0.84 +0.9 160,417 + EX28DRAFT_0205 0.86 -0.6 160,418 - EX28DRAFT_0205 0.86 -1.6 160,418 - EX28DRAFT_0205 0.86 -1.3 160,436 + EX28DRAFT_0205 0.88 -0.6 160,437 - EX28DRAFT_0205 0.88 +1.9 160,528 + -2.1 160,553 + -0.5 160,553 + -1.1 160,553 + -4.1 160,554 - -1.8 160,557 + -1.5 160,557 + -0.4 160,558 - +0.2 160,558 - -0.1 160,603 - -1.8 160,738 + EX28DRAFT_0206 0.21 -0.8 160,738 + EX28DRAFT_0206 0.21 -1.1 160,739 - EX28DRAFT_0206 0.22 -1.1 160,970 + EX28DRAFT_0206 0.49 -0.5 160,970 + EX28DRAFT_0206 0.49 -1.3 160,970 + EX28DRAFT_0206 0.49 -1.1 160,971 - EX28DRAFT_0206 0.49 -0.9 161,382 + +1.0 161,382 + -2.0 161,383 - -1.1 161,383 - -0.5 161,404 - -0.3 161,509 + EX28DRAFT_0207 0.13 -2.1 161,509 + EX28DRAFT_0207 0.13 -1.1 161,510 - EX28DRAFT_0207 0.13 -2.1 161,510 - EX28DRAFT_0207 0.13 -1.2 161,511 + EX28DRAFT_0207 0.14 -0.6 161,512 - EX28DRAFT_0207 0.14 -1.5 161,753 - EX28DRAFT_0207 0.41 +0.1 161,764 + EX28DRAFT_0207 0.42 -0.9 161,765 - EX28DRAFT_0207 0.43 -4.9 161,765 - EX28DRAFT_0207 0.43 -1.2 161,805 + EX28DRAFT_0207 0.47 -0.6 162,004 + EX28DRAFT_0207 0.70 -0.5 162,128 - EX28DRAFT_0207 0.84 -1.2 162,278 - -2.4 162,280 + -0.2 162,280 + -0.3 162,281 - -2.9 162,316 + +0.2 162,478 + EX28DRAFT_0208 0.20 -0.3 162,493 - EX28DRAFT_0208 0.22 -2.3 162,503 + EX28DRAFT_0208 0.22 -1.0 162,992 - EX28DRAFT_0208 0.67 -0.8 163,169 + EX28DRAFT_0208 0.83 -1.8 163,169 + EX28DRAFT_0208 0.83 -1.3 163,186 - EX28DRAFT_0208 0.85 +0.4
Or see this region's nucleotide sequence