Strain Fitness in Enterobacter asburiae PDN3 around EX28DRAFT_0111

Experiment: M9 with sucrose and trichloroethylene 76.3 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX28DRAFT_0110 and EX28DRAFT_0111 are separated by 24 nucleotidesEX28DRAFT_0111 and EX28DRAFT_0112 are separated by 13 nucleotidesEX28DRAFT_0112 and EX28DRAFT_0113 are separated by 80 nucleotidesEX28DRAFT_0113 and EX28DRAFT_0114 are separated by 14 nucleotides EX28DRAFT_0110: EX28DRAFT_0110 - cysteine desulfurase IscS, at 49,472 to 50,686 _0110 EX28DRAFT_0111: EX28DRAFT_0111 - FeS cluster assembly scaffold IscU, at 50,711 to 51,097 _0111 EX28DRAFT_0112: EX28DRAFT_0112 - iron-sulfur cluster assembly protein IscA, at 51,111 to 51,434 _0112 EX28DRAFT_0113: EX28DRAFT_0113 - Fe-S protein assembly co-chaperone HscB, at 51,515 to 52,030 _0113 EX28DRAFT_0114: EX28DRAFT_0114 - Fe-S protein assembly chaperone HscA, at 52,045 to 53,895 _0114 Position (kb) 50 51 52Strain fitness (log2 ratio) -2 -1 0 1at 50.863 kb on + strand, within EX28DRAFT_0111at 51.521 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM
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50,863 + EX28DRAFT_0111 0.39 -2.2
51,521 + -1.7

Or see this region's nucleotide sequence