Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0090 and EX28DRAFT_0091 are separated by 135 nucleotides EX28DRAFT_0091 and EX28DRAFT_0092 are separated by 30 nucleotides EX28DRAFT_0092 and EX28DRAFT_0093 are separated by 291 nucleotides EX28DRAFT_0093 and EX28DRAFT_0094 are separated by 96 nucleotides
EX28DRAFT_0090: EX28DRAFT_0090 - Predicted soluble lytic transglycosylase fused to an ABC-type amino acid-binding protein, at 28,574 to 30,124
_0090
EX28DRAFT_0091: EX28DRAFT_0091 - hypothetical protein, at 30,260 to 30,361
_0091
EX28DRAFT_0092: EX28DRAFT_0092 - phosphoribosylformylglycinamidine synthase, single chain form, at 30,392 to 34,279
_0092
EX28DRAFT_0093: EX28DRAFT_0093 - RNA, at 34,571 to 34,717
_0093
EX28DRAFT_0094: EX28DRAFT_0094 - Signal transduction histidine kinase, at 34,814 to 36,241
_0094
Position (kb) 30
31
32
33
34
35 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 29.529 kb on + strand, within EX28DRAFT_0090 at 29.530 kb on - strand, within EX28DRAFT_0090 at 29.683 kb on + strand, within EX28DRAFT_0090 at 29.749 kb on - strand, within EX28DRAFT_0090 at 29.751 kb on + strand, within EX28DRAFT_0090 at 29.752 kb on - strand, within EX28DRAFT_0090 at 29.769 kb on + strand, within EX28DRAFT_0090 at 29.791 kb on + strand, within EX28DRAFT_0090 at 29.793 kb on + strand, within EX28DRAFT_0090 at 29.793 kb on + strand, within EX28DRAFT_0090 at 29.794 kb on - strand, within EX28DRAFT_0090 at 29.794 kb on - strand, within EX28DRAFT_0090 at 29.858 kb on - strand, within EX28DRAFT_0090 at 30.084 kb on - strand at 30.174 kb on + strand at 30.239 kb on + strand at 30.258 kb on + strand at 30.259 kb on - strand at 30.325 kb on + strand, within EX28DRAFT_0091 at 30.500 kb on + strand at 31.011 kb on - strand, within EX28DRAFT_0092 at 31.157 kb on + strand, within EX28DRAFT_0092 at 32.043 kb on - strand, within EX28DRAFT_0092 at 32.591 kb on - strand, within EX28DRAFT_0092 at 34.409 kb on + strand at 34.444 kb on + strand at 34.445 kb on - strand at 34.458 kb on - strand at 34.590 kb on + strand, within EX28DRAFT_0093 at 34.591 kb on - strand, within EX28DRAFT_0093 at 34.591 kb on - strand, within EX28DRAFT_0093 at 34.615 kb on - strand, within EX28DRAFT_0093 at 34.618 kb on + strand, within EX28DRAFT_0093 at 34.619 kb on - strand, within EX28DRAFT_0093 at 34.659 kb on + strand, within EX28DRAFT_0093 at 34.659 kb on + strand, within EX28DRAFT_0093 at 34.660 kb on - strand, within EX28DRAFT_0093 at 34.660 kb on - strand, within EX28DRAFT_0093 at 34.679 kb on + strand, within EX28DRAFT_0093 at 34.705 kb on + strand at 34.756 kb on + strand at 34.808 kb on - strand at 35.023 kb on + strand, within EX28DRAFT_0094 at 35.023 kb on + strand, within EX28DRAFT_0094 at 35.023 kb on + strand, within EX28DRAFT_0094 at 35.023 kb on + strand, within EX28DRAFT_0094 at 35.027 kb on + strand, within EX28DRAFT_0094 at 35.027 kb on + strand, within EX28DRAFT_0094 at 35.028 kb on - strand, within EX28DRAFT_0094 at 35.028 kb on - strand, within EX28DRAFT_0094 at 35.054 kb on - strand, within EX28DRAFT_0094 at 35.072 kb on + strand, within EX28DRAFT_0094 at 35.073 kb on - strand, within EX28DRAFT_0094 at 35.086 kb on + strand, within EX28DRAFT_0094
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 29,529 + EX28DRAFT_0090 0.62 +0.5 29,530 - EX28DRAFT_0090 0.62 +0.4 29,683 + EX28DRAFT_0090 0.72 -0.2 29,749 - EX28DRAFT_0090 0.76 -0.4 29,751 + EX28DRAFT_0090 0.76 -0.0 29,752 - EX28DRAFT_0090 0.76 +0.5 29,769 + EX28DRAFT_0090 0.77 +0.0 29,791 + EX28DRAFT_0090 0.78 -0.5 29,793 + EX28DRAFT_0090 0.79 -0.2 29,793 + EX28DRAFT_0090 0.79 +0.5 29,794 - EX28DRAFT_0090 0.79 -0.9 29,794 - EX28DRAFT_0090 0.79 +0.4 29,858 - EX28DRAFT_0090 0.83 -0.6 30,084 - +0.4 30,174 + -0.4 30,239 + -0.5 30,258 + +0.5 30,259 - -1.7 30,325 + EX28DRAFT_0091 0.64 +1.1 30,500 + -2.0 31,011 - EX28DRAFT_0092 0.16 -1.7 31,157 + EX28DRAFT_0092 0.20 -1.7 32,043 - EX28DRAFT_0092 0.42 -2.1 32,591 - EX28DRAFT_0092 0.57 -3.3 34,409 + -2.4 34,444 + +0.3 34,445 - +0.2 34,458 - -1.5 34,590 + EX28DRAFT_0093 0.13 -0.4 34,591 - EX28DRAFT_0093 0.14 -0.9 34,591 - EX28DRAFT_0093 0.14 +0.2 34,615 - EX28DRAFT_0093 0.30 -1.2 34,618 + EX28DRAFT_0093 0.32 +0.3 34,619 - EX28DRAFT_0093 0.33 +0.1 34,659 + EX28DRAFT_0093 0.60 +0.2 34,659 + EX28DRAFT_0093 0.60 +0.8 34,660 - EX28DRAFT_0093 0.61 -0.7 34,660 - EX28DRAFT_0093 0.61 -0.2 34,679 + EX28DRAFT_0093 0.73 -0.0 34,705 + -1.1 34,756 + -0.3 34,808 - +0.3 35,023 + EX28DRAFT_0094 0.15 +1.0 35,023 + EX28DRAFT_0094 0.15 +0.2 35,023 + EX28DRAFT_0094 0.15 -0.1 35,023 + EX28DRAFT_0094 0.15 -0.6 35,027 + EX28DRAFT_0094 0.15 +1.0 35,027 + EX28DRAFT_0094 0.15 +0.2 35,028 - EX28DRAFT_0094 0.15 +0.2 35,028 - EX28DRAFT_0094 0.15 +0.1 35,054 - EX28DRAFT_0094 0.17 -0.3 35,072 + EX28DRAFT_0094 0.18 +0.1 35,073 - EX28DRAFT_0094 0.18 -2.5 35,086 + EX28DRAFT_0094 0.19 -0.3
Or see this region's nucleotide sequence