Strain Fitness in Enterobacter asburiae PDN3 around EX28DRAFT_0081

Experiment: M9 with sucrose and trichloroethylene 76.3 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX28DRAFT_0079 and EX28DRAFT_0080 overlap by 4 nucleotidesEX28DRAFT_0080 and EX28DRAFT_0081 are separated by 17 nucleotidesEX28DRAFT_0081 and EX28DRAFT_0082 are separated by 75 nucleotidesEX28DRAFT_0082 and EX28DRAFT_0083 overlap by 1 nucleotides EX28DRAFT_0079: EX28DRAFT_0079 - ribonuclease III, bacterial, at 20,453 to 21,133 _0079 EX28DRAFT_0080: EX28DRAFT_0080 - GTP-binding protein Era, at 21,130 to 22,035 _0080 EX28DRAFT_0081: EX28DRAFT_0081 - DNA replication and repair protein RecO, at 22,053 to 22,760 _0081 EX28DRAFT_0082: EX28DRAFT_0082 - pyridoxine 5'-phosphate synthase, at 22,836 to 23,567 _0082 EX28DRAFT_0083: EX28DRAFT_0083 - holo-[acyl-carrier-protein] synthase, at 23,567 to 23,947 _0083 Position (kb) 22 23Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 22.038 kb on + strandat 22.038 kb on + strandat 22.052 kb on + strandat 22.086 kb on - strandat 22.086 kb on - strandat 22.086 kb on - strandat 22.113 kb on - strandat 22.225 kb on + strand, within EX28DRAFT_0081at 22.323 kb on + strand, within EX28DRAFT_0081at 22.489 kb on + strand, within EX28DRAFT_0081at 22.526 kb on - strand, within EX28DRAFT_0081at 22.626 kb on + strand, within EX28DRAFT_0081at 22.626 kb on + strand, within EX28DRAFT_0081at 22.886 kb on + strandat 23.376 kb on + strand, within EX28DRAFT_0082

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM
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22,038 + +0.0
22,038 + -2.1
22,052 + +0.1
22,086 - +0.3
22,086 - -1.3
22,086 - +0.4
22,113 - -1.2
22,225 + EX28DRAFT_0081 0.24 -4.3
22,323 + EX28DRAFT_0081 0.38 -1.2
22,489 + EX28DRAFT_0081 0.62 -0.1
22,526 - EX28DRAFT_0081 0.67 +0.0
22,626 + EX28DRAFT_0081 0.81 -4.3
22,626 + EX28DRAFT_0081 0.81 -1.1
22,886 + -3.7
23,376 + EX28DRAFT_0082 0.74 -1.1

Or see this region's nucleotide sequence