Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0074 and EX28DRAFT_0075 overlap by 4 nucleotides EX28DRAFT_0075 and EX28DRAFT_0076 are separated by 185 nucleotides EX28DRAFT_0076 and EX28DRAFT_0077 are separated by 15 nucleotides
EX28DRAFT_0074: EX28DRAFT_0074 - sigma E regulatory protein, MucB/RseB, at 15,829 to 16,782
_0074
EX28DRAFT_0075: EX28DRAFT_0075 - positive regulator of sigma(E), RseC/MucC, at 16,779 to 17,255
_0075
EX28DRAFT_0076: EX28DRAFT_0076 - elongation factor 4, at 17,441 to 19,240
_0076
EX28DRAFT_0077: EX28DRAFT_0077 - signal peptidase I, bacterial type, at 19,256 to 20,230
_0077
Position (kb)
17
18
19
20 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 16.482 kb on + strand, within EX28DRAFT_0074 at 16.585 kb on + strand, within EX28DRAFT_0074 at 16.618 kb on - strand, within EX28DRAFT_0074 at 16.680 kb on + strand, within EX28DRAFT_0074 at 16.681 kb on - strand, within EX28DRAFT_0074 at 16.761 kb on + strand at 16.761 kb on + strand at 16.857 kb on - strand, within EX28DRAFT_0075 at 16.857 kb on - strand, within EX28DRAFT_0075 at 16.860 kb on - strand, within EX28DRAFT_0075 at 16.902 kb on - strand, within EX28DRAFT_0075 at 17.022 kb on + strand, within EX28DRAFT_0075 at 17.048 kb on + strand, within EX28DRAFT_0075 at 17.048 kb on + strand, within EX28DRAFT_0075 at 17.096 kb on + strand, within EX28DRAFT_0075 at 17.315 kb on + strand at 17.316 kb on - strand at 17.325 kb on + strand at 17.326 kb on - strand at 17.326 kb on - strand at 17.326 kb on - strand at 17.326 kb on - strand at 17.352 kb on + strand at 17.352 kb on + strand at 17.352 kb on + strand at 17.357 kb on + strand at 17.430 kb on + strand at 17.509 kb on + strand at 17.627 kb on + strand, within EX28DRAFT_0076 at 18.094 kb on + strand, within EX28DRAFT_0076 at 18.554 kb on + strand, within EX28DRAFT_0076 at 18.767 kb on + strand, within EX28DRAFT_0076 at 19.048 kb on + strand, within EX28DRAFT_0076 at 20.228 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 16,482 + EX28DRAFT_0074 0.68 +0.2 16,585 + EX28DRAFT_0074 0.79 +0.3 16,618 - EX28DRAFT_0074 0.83 -1.1 16,680 + EX28DRAFT_0074 0.89 +0.2 16,681 - EX28DRAFT_0074 0.89 -0.7 16,761 + +0.9 16,761 + -0.4 16,857 - EX28DRAFT_0075 0.16 +0.8 16,857 - EX28DRAFT_0075 0.16 +1.1 16,860 - EX28DRAFT_0075 0.17 -1.8 16,902 - EX28DRAFT_0075 0.26 -0.0 17,022 + EX28DRAFT_0075 0.51 -0.0 17,048 + EX28DRAFT_0075 0.56 +3.6 17,048 + EX28DRAFT_0075 0.56 -0.1 17,096 + EX28DRAFT_0075 0.66 -0.0 17,315 + -0.0 17,316 - +0.1 17,325 + -2.2 17,326 - -0.4 17,326 - +0.6 17,326 - -1.9 17,326 - +0.5 17,352 + +0.1 17,352 + -0.1 17,352 + -0.9 17,357 + -0.2 17,430 + +0.5 17,509 + -2.4 17,627 + EX28DRAFT_0076 0.10 -1.0 18,094 + EX28DRAFT_0076 0.36 +0.2 18,554 + EX28DRAFT_0076 0.62 -0.7 18,767 + EX28DRAFT_0076 0.74 -1.7 19,048 + EX28DRAFT_0076 0.89 -1.1 20,228 + -2.7
Or see this region's nucleotide sequence