Experiment: L-Arginine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yhfG and ppiA are separated by 104 nucleotides ppiA and tsgA are separated by 270 nucleotides tsgA and nirB are separated by 261 nucleotides
b3362: yhfG - hypothetical protein (NCBI), at 3,489,475 to 3,489,642
yhfG
b3363: ppiA - peptidyl-prolyl cis-trans isomerase A (rotamase A) (NCBI), at 3,489,747 to 3,490,319
ppiA
b3364: tsgA - hypothetical protein (NCBI), at 3,490,590 to 3,491,771
tsgA
b3365: nirB - nitrite reductase, large subunit, NAD(P)H-binding (NCBI), at 3,492,033 to 3,494,576
nirB
Position (kb)
3490
3491
3492 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6
7
8
9
10 at 3489.659 kb on + strand at 3489.659 kb on - strand at 3489.676 kb on + strand at 3489.700 kb on + strand at 3489.700 kb on + strand at 3489.743 kb on - strand at 3489.743 kb on - strand at 3489.753 kb on - strand at 3489.753 kb on - strand at 3489.804 kb on - strand at 3489.804 kb on - strand at 3489.804 kb on - strand at 3489.804 kb on - strand at 3489.806 kb on + strand, within ppiA at 3489.806 kb on + strand, within ppiA at 3489.820 kb on - strand, within ppiA at 3489.820 kb on - strand, within ppiA at 3489.849 kb on + strand, within ppiA at 3489.849 kb on + strand, within ppiA at 3489.849 kb on + strand, within ppiA at 3489.857 kb on - strand, within ppiA at 3489.866 kb on - strand, within ppiA at 3489.866 kb on - strand, within ppiA at 3489.878 kb on - strand, within ppiA at 3489.878 kb on - strand, within ppiA at 3489.905 kb on + strand, within ppiA at 3489.905 kb on + strand, within ppiA at 3489.914 kb on - strand, within ppiA at 3489.916 kb on - strand, within ppiA at 3489.916 kb on - strand, within ppiA at 3489.967 kb on - strand, within ppiA at 3489.967 kb on - strand, within ppiA at 3489.989 kb on + strand, within ppiA at 3489.997 kb on - strand, within ppiA at 3489.997 kb on - strand, within ppiA at 3490.055 kb on + strand, within ppiA at 3490.115 kb on - strand, within ppiA at 3490.120 kb on + strand, within ppiA at 3490.120 kb on + strand, within ppiA at 3490.188 kb on - strand, within ppiA at 3490.201 kb on + strand, within ppiA at 3490.226 kb on - strand, within ppiA at 3490.261 kb on + strand, within ppiA at 3490.267 kb on + strand at 3490.267 kb on + strand at 3490.269 kb on + strand at 3490.348 kb on + strand at 3490.348 kb on + strand at 3490.355 kb on + strand at 3490.367 kb on + strand at 3490.367 kb on + strand at 3490.377 kb on + strand at 3490.428 kb on - strand at 3490.555 kb on - strand at 3490.555 kb on - strand at 3490.590 kb on - strand at 3490.590 kb on - strand at 3490.598 kb on + strand at 3490.607 kb on + strand at 3490.607 kb on + strand at 3490.634 kb on + strand at 3490.636 kb on + strand at 3490.636 kb on + strand at 3490.644 kb on - strand at 3490.651 kb on + strand at 3490.661 kb on - strand at 3490.724 kb on + strand, within tsgA at 3490.726 kb on + strand, within tsgA at 3490.728 kb on - strand, within tsgA at 3490.731 kb on + strand, within tsgA at 3490.731 kb on + strand, within tsgA at 3490.734 kb on - strand, within tsgA at 3490.762 kb on + strand, within tsgA at 3490.787 kb on + strand, within tsgA at 3490.787 kb on + strand, within tsgA at 3490.847 kb on - strand, within tsgA at 3490.847 kb on - strand, within tsgA at 3490.847 kb on - strand, within tsgA at 3490.881 kb on - strand, within tsgA at 3490.929 kb on - strand, within tsgA at 3490.929 kb on - strand, within tsgA at 3490.947 kb on - strand, within tsgA at 3491.022 kb on + strand, within tsgA at 3491.022 kb on - strand, within tsgA at 3491.065 kb on + strand, within tsgA at 3491.065 kb on + strand, within tsgA at 3491.068 kb on - strand, within tsgA at 3491.068 kb on - strand, within tsgA at 3491.073 kb on - strand, within tsgA at 3491.073 kb on - strand, within tsgA at 3491.073 kb on - strand, within tsgA at 3491.075 kb on + strand, within tsgA at 3491.075 kb on + strand, within tsgA at 3491.075 kb on + strand, within tsgA at 3491.083 kb on - strand, within tsgA at 3491.179 kb on + strand, within tsgA at 3491.181 kb on + strand, within tsgA at 3491.234 kb on - strand, within tsgA at 3491.253 kb on - strand, within tsgA at 3491.253 kb on - strand, within tsgA at 3491.263 kb on - strand, within tsgA at 3491.274 kb on + strand, within tsgA at 3491.274 kb on + strand, within tsgA at 3491.280 kb on + strand, within tsgA at 3491.280 kb on + strand, within tsgA at 3491.304 kb on + strand, within tsgA at 3491.315 kb on + strand, within tsgA at 3491.367 kb on - strand, within tsgA at 3491.367 kb on - strand, within tsgA at 3491.375 kb on - strand, within tsgA at 3491.382 kb on - strand, within tsgA at 3491.402 kb on - strand, within tsgA at 3491.420 kb on + strand, within tsgA at 3491.420 kb on + strand, within tsgA at 3491.468 kb on + strand, within tsgA at 3491.470 kb on + strand, within tsgA at 3491.472 kb on + strand, within tsgA at 3491.517 kb on + strand, within tsgA at 3491.517 kb on + strand, within tsgA at 3491.517 kb on + strand, within tsgA at 3491.526 kb on + strand, within tsgA at 3491.540 kb on + strand, within tsgA at 3491.542 kb on + strand, within tsgA at 3491.542 kb on + strand, within tsgA at 3491.550 kb on - strand, within tsgA at 3491.550 kb on - strand, within tsgA at 3491.550 kb on - strand, within tsgA at 3491.554 kb on + strand, within tsgA at 3491.555 kb on - strand, within tsgA at 3491.603 kb on + strand, within tsgA at 3491.603 kb on + strand, within tsgA at 3491.621 kb on - strand, within tsgA at 3491.621 kb on - strand, within tsgA at 3491.646 kb on + strand, within tsgA at 3491.646 kb on + strand, within tsgA at 3491.646 kb on + strand, within tsgA at 3491.666 kb on - strand at 3491.675 kb on - strand at 3491.726 kb on - strand at 3491.726 kb on - strand at 3491.726 kb on - strand at 3491.747 kb on + strand at 3491.747 kb on + strand at 3491.755 kb on - strand at 3491.755 kb on - strand at 3491.801 kb on - strand at 3491.814 kb on + strand at 3491.814 kb on - strand at 3491.841 kb on + strand at 3491.841 kb on + strand at 3491.993 kb on + strand at 3491.993 kb on + strand at 3492.065 kb on + strand at 3492.065 kb on + strand at 3492.065 kb on + strand at 3492.073 kb on - strand at 3492.159 kb on + strand at 3492.159 kb on + strand at 3492.159 kb on + strand at 3492.167 kb on - strand at 3492.176 kb on - strand at 3492.198 kb on - strand at 3492.220 kb on - strand at 3492.275 kb on + strand at 3492.279 kb on + strand at 3492.316 kb on + strand, within nirB at 3492.319 kb on - strand, within nirB at 3492.352 kb on - strand, within nirB at 3492.355 kb on - strand, within nirB at 3492.361 kb on + strand, within nirB at 3492.424 kb on + strand, within nirB at 3492.424 kb on + strand, within nirB at 3492.434 kb on - strand, within nirB at 3492.444 kb on + strand, within nirB at 3492.495 kb on - strand, within nirB at 3492.495 kb on - strand, within nirB at 3492.574 kb on + strand, within nirB at 3492.574 kb on + strand, within nirB at 3492.599 kb on - strand, within nirB at 3492.647 kb on - strand, within nirB at 3492.677 kb on - strand, within nirB at 3492.680 kb on - strand, within nirB at 3492.690 kb on - strand, within nirB at 3492.765 kb on - strand, within nirB at 3492.765 kb on - strand, within nirB
Per-strain Table
Position Strand Gene LocusTag Fraction L-Arginine (N) remove 3,489,659 + -1.0 3,489,659 - -0.1 3,489,676 + +1.5 3,489,700 + -0.2 3,489,700 + +0.8 3,489,743 - +0.1 3,489,743 - -1.0 3,489,753 - -0.0 3,489,753 - -0.6 3,489,804 - -0.5 3,489,804 - +0.8 3,489,804 - -1.2 3,489,804 - +0.3 3,489,806 + ppiA b3363 0.10 -1.1 3,489,806 + ppiA b3363 0.10 +1.4 3,489,820 - ppiA b3363 0.13 +0.4 3,489,820 - ppiA b3363 0.13 +2.4 3,489,849 + ppiA b3363 0.18 +1.0 3,489,849 + ppiA b3363 0.18 +1.0 3,489,849 + ppiA b3363 0.18 -0.4 3,489,857 - ppiA b3363 0.19 -0.7 3,489,866 - ppiA b3363 0.21 -0.0 3,489,866 - ppiA b3363 0.21 -0.6 3,489,878 - ppiA b3363 0.23 +0.1 3,489,878 - ppiA b3363 0.23 +0.9 3,489,905 + ppiA b3363 0.28 +1.4 3,489,905 + ppiA b3363 0.28 +0.3 3,489,914 - ppiA b3363 0.29 -1.8 3,489,916 - ppiA b3363 0.29 -0.0 3,489,916 - ppiA b3363 0.29 +0.8 3,489,967 - ppiA b3363 0.38 +1.9 3,489,967 - ppiA b3363 0.38 -1.1 3,489,989 + ppiA b3363 0.42 +1.0 3,489,997 - ppiA b3363 0.44 +0.1 3,489,997 - ppiA b3363 0.44 -0.2 3,490,055 + ppiA b3363 0.54 +0.6 3,490,115 - ppiA b3363 0.64 -0.5 3,490,120 + ppiA b3363 0.65 +0.3 3,490,120 + ppiA b3363 0.65 +1.1 3,490,188 - ppiA b3363 0.77 -0.2 3,490,201 + ppiA b3363 0.79 +0.4 3,490,226 - ppiA b3363 0.84 -1.2 3,490,261 + ppiA b3363 0.90 +0.3 3,490,267 + +0.4 3,490,267 + -0.4 3,490,269 + +0.4 3,490,348 + +0.3 3,490,348 + +0.6 3,490,355 + -0.4 3,490,367 + +0.3 3,490,367 + -2.2 3,490,377 + +0.5 3,490,428 - +0.5 3,490,555 - -0.2 3,490,555 - -0.7 3,490,590 - -1.4 3,490,590 - -0.1 3,490,598 + +0.4 3,490,607 + +0.6 3,490,607 + -0.3 3,490,634 + -0.9 3,490,636 + +0.3 3,490,636 + +0.1 3,490,644 - +1.1 3,490,651 + -0.3 3,490,661 - -0.7 3,490,724 + tsgA b3364 0.11 -0.2 3,490,726 + tsgA b3364 0.12 +0.4 3,490,728 - tsgA b3364 0.12 -0.3 3,490,731 + tsgA b3364 0.12 +0.5 3,490,731 + tsgA b3364 0.12 +0.6 3,490,734 - tsgA b3364 0.12 +0.7 3,490,762 + tsgA b3364 0.15 -0.7 3,490,787 + tsgA b3364 0.17 +0.7 3,490,787 + tsgA b3364 0.17 -0.3 3,490,847 - tsgA b3364 0.22 -0.6 3,490,847 - tsgA b3364 0.22 -0.7 3,490,847 - tsgA b3364 0.22 -1.1 3,490,881 - tsgA b3364 0.25 +1.7 3,490,929 - tsgA b3364 0.29 +2.6 3,490,929 - tsgA b3364 0.29 +0.3 3,490,947 - tsgA b3364 0.30 +0.1 3,491,022 + tsgA b3364 0.37 -1.7 3,491,022 - tsgA b3364 0.37 +0.5 3,491,065 + tsgA b3364 0.40 +0.4 3,491,065 + tsgA b3364 0.40 +0.3 3,491,068 - tsgA b3364 0.40 +0.7 3,491,068 - tsgA b3364 0.40 -0.7 3,491,073 - tsgA b3364 0.41 +0.6 3,491,073 - tsgA b3364 0.41 -0.1 3,491,073 - tsgA b3364 0.41 +0.4 3,491,075 + tsgA b3364 0.41 +0.3 3,491,075 + tsgA b3364 0.41 -0.2 3,491,075 + tsgA b3364 0.41 +0.1 3,491,083 - tsgA b3364 0.42 -0.2 3,491,179 + tsgA b3364 0.50 -0.0 3,491,181 + tsgA b3364 0.50 +1.5 3,491,234 - tsgA b3364 0.54 -0.0 3,491,253 - tsgA b3364 0.56 -0.2 3,491,253 - tsgA b3364 0.56 -0.2 3,491,263 - tsgA b3364 0.57 +0.8 3,491,274 + tsgA b3364 0.58 +1.1 3,491,274 + tsgA b3364 0.58 +1.1 3,491,280 + tsgA b3364 0.58 +1.0 3,491,280 + tsgA b3364 0.58 +0.0 3,491,304 + tsgA b3364 0.60 +0.5 3,491,315 + tsgA b3364 0.61 -0.0 3,491,367 - tsgA b3364 0.66 +0.7 3,491,367 - tsgA b3364 0.66 -0.9 3,491,375 - tsgA b3364 0.66 +0.9 3,491,382 - tsgA b3364 0.67 -1.3 3,491,402 - tsgA b3364 0.69 +0.1 3,491,420 + tsgA b3364 0.70 -1.2 3,491,420 + tsgA b3364 0.70 -2.0 3,491,468 + tsgA b3364 0.74 -1.1 3,491,470 + tsgA b3364 0.74 -0.7 3,491,472 + tsgA b3364 0.75 -0.1 3,491,517 + tsgA b3364 0.78 +0.7 3,491,517 + tsgA b3364 0.78 +0.6 3,491,517 + tsgA b3364 0.78 +1.1 3,491,526 + tsgA b3364 0.79 -0.2 3,491,540 + tsgA b3364 0.80 -1.0 3,491,542 + tsgA b3364 0.81 +0.9 3,491,542 + tsgA b3364 0.81 +0.6 3,491,550 - tsgA b3364 0.81 -0.9 3,491,550 - tsgA b3364 0.81 +0.6 3,491,550 - tsgA b3364 0.81 +0.7 3,491,554 + tsgA b3364 0.82 +0.6 3,491,555 - tsgA b3364 0.82 -0.8 3,491,603 + tsgA b3364 0.86 +1.3 3,491,603 + tsgA b3364 0.86 +3.0 3,491,621 - tsgA b3364 0.87 +9.9 3,491,621 - tsgA b3364 0.87 +8.6 3,491,646 + tsgA b3364 0.89 -0.7 3,491,646 + tsgA b3364 0.89 +1.5 3,491,646 + tsgA b3364 0.89 +0.2 3,491,666 - -0.8 3,491,675 - +1.1 3,491,726 - -0.4 3,491,726 - +1.8 3,491,726 - +2.4 3,491,747 + +0.6 3,491,747 + +0.1 3,491,755 - +0.5 3,491,755 - -0.4 3,491,801 - +0.3 3,491,814 + -0.6 3,491,814 - +0.6 3,491,841 + +2.4 3,491,841 + +0.8 3,491,993 + -2.0 3,491,993 + +0.4 3,492,065 + +0.3 3,492,065 + -0.6 3,492,065 + +0.2 3,492,073 - +0.2 3,492,159 + +2.6 3,492,159 + -1.2 3,492,159 + -0.2 3,492,167 - +0.3 3,492,176 - +0.3 3,492,198 - +0.6 3,492,220 - +0.4 3,492,275 + -0.9 3,492,279 + +2.1 3,492,316 + nirB b3365 0.11 +0.6 3,492,319 - nirB b3365 0.11 -0.8 3,492,352 - nirB b3365 0.13 -0.3 3,492,355 - nirB b3365 0.13 +0.1 3,492,361 + nirB b3365 0.13 +0.4 3,492,424 + nirB b3365 0.15 -0.8 3,492,424 + nirB b3365 0.15 -0.0 3,492,434 - nirB b3365 0.16 -1.0 3,492,444 + nirB b3365 0.16 -0.3 3,492,495 - nirB b3365 0.18 +0.4 3,492,495 - nirB b3365 0.18 +1.4 3,492,574 + nirB b3365 0.21 +0.3 3,492,574 + nirB b3365 0.21 -0.5 3,492,599 - nirB b3365 0.22 -0.1 3,492,647 - nirB b3365 0.24 +0.4 3,492,677 - nirB b3365 0.25 -0.8 3,492,680 - nirB b3365 0.25 +0.5 3,492,690 - nirB b3365 0.26 +1.0 3,492,765 - nirB b3365 0.29 -1.2 3,492,765 - nirB b3365 0.29 -1.0
Or see this region's nucleotide sequence