Experiment: CPG
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt UW163_RS13815 and UW163_RS13820 are separated by 19 nucleotides UW163_RS13820 and UW163_RS13825 are separated by 121 nucleotides UW163_RS13825 and UW163_RS13830 overlap by 14 nucleotides UW163_RS13830 and UW163_RS13835 are separated by 5 nucleotides
UW163_RS13815: UW163_RS13815 - SPFH/Band 7/PHB domain protein, at 2,975,338 to 2,976,264
_RS13815
UW163_RS13820: UW163_RS13820 - SsrA-binding protein SmpB, at 2,976,284 to 2,976,730
_RS13820
UW163_RS13825: UW163_RS13825 - ubiquinone-binding protein, at 2,976,852 to 2,977,289
_RS13825
UW163_RS13830: UW163_RS13830 - hypothetical protein, at 2,977,276 to 2,977,632
_RS13830
UW163_RS13835: UW163_RS13835 - DUF4124 domain-containing protein, at 2,977,638 to 2,978,177
_RS13835
Position (kb)
2976
2977
2978 Strain fitness (log2 ratio)
-1
0
1 at 2976.136 kb on + strand, within UW163_RS13815 at 2976.136 kb on + strand, within UW163_RS13815 at 2976.137 kb on - strand, within UW163_RS13815 at 2976.930 kb on + strand, within UW163_RS13825 at 2977.298 kb on - strand at 2977.444 kb on + strand, within UW163_RS13830 at 2977.709 kb on + strand, within UW163_RS13835 at 2977.711 kb on + strand, within UW163_RS13835 at 2977.711 kb on + strand, within UW163_RS13835 at 2977.712 kb on - strand, within UW163_RS13835 at 2977.765 kb on + strand, within UW163_RS13835 at 2978.281 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction CPG remove 2,976,136 + UW163_RS13815 0.86 -0.4 2,976,136 + UW163_RS13815 0.86 -0.1 2,976,137 - UW163_RS13815 0.86 +0.2 2,976,930 + UW163_RS13825 0.18 +1.5 2,977,298 - +0.6 2,977,444 + UW163_RS13830 0.47 -0.0 2,977,709 + UW163_RS13835 0.13 -0.4 2,977,711 + UW163_RS13835 0.14 -0.1 2,977,711 + UW163_RS13835 0.14 -1.1 2,977,712 - UW163_RS13835 0.14 -0.8 2,977,765 + UW163_RS13835 0.24 +0.1 2,978,281 - -0.4
Or see this region's nucleotide sequence