Experiment: CPG
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt UW163_RS01005 and UW163_RS01010 are separated by 12 nucleotides UW163_RS01010 and UW163_RS01015 are separated by 15 nucleotides UW163_RS01015 and UW163_RS01020 are separated by 641 nucleotides
UW163_RS01005: UW163_RS01005 - LemA family protein, at 201,567 to 202,193
_RS01005
UW163_RS01010: UW163_RS01010 - membrane protein, at 202,206 to 203,057
_RS01010
UW163_RS01015: UW163_RS01015 - hypothetical protein, at 203,073 to 203,594
_RS01015
UW163_RS01020: UW163_RS01020 - Flp family type IVb pilin, at 204,236 to 204,421
_RS01020
Position (kb) 203
204 Strain fitness (log2 ratio) -1
0
1 at 202.123 kb on - strand, within UW163_RS01005 at 202.282 kb on - strand at 202.392 kb on + strand, within UW163_RS01010 at 202.671 kb on + strand, within UW163_RS01010 at 202.671 kb on + strand, within UW163_RS01010 at 203.055 kb on + strand at 203.355 kb on + strand, within UW163_RS01015 at 203.355 kb on + strand, within UW163_RS01015 at 203.706 kb on - strand at 203.706 kb on - strand at 203.771 kb on + strand at 204.120 kb on + strand at 204.121 kb on - strand at 204.121 kb on - strand at 204.121 kb on - strand at 204.147 kb on - strand at 204.294 kb on - strand, within UW163_RS01020 at 204.393 kb on + strand, within UW163_RS01020 at 204.393 kb on + strand, within UW163_RS01020 at 204.393 kb on + strand, within UW163_RS01020 at 204.420 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction CPG remove 202,123 - UW163_RS01005 0.89 +1.1 202,282 - +0.5 202,392 + UW163_RS01010 0.22 -0.0 202,671 + UW163_RS01010 0.55 -0.1 202,671 + UW163_RS01010 0.55 -0.0 203,055 + -0.3 203,355 + UW163_RS01015 0.54 -0.5 203,355 + UW163_RS01015 0.54 -0.0 203,706 - +0.3 203,706 - +0.6 203,771 + -0.3 204,120 + +1.0 204,121 - -0.1 204,121 - -0.2 204,121 - -0.1 204,147 - -0.4 204,294 - UW163_RS01020 0.31 +0.2 204,393 + UW163_RS01020 0.84 -1.0 204,393 + UW163_RS01020 0.84 -0.7 204,393 + UW163_RS01020 0.84 +0.3 204,420 - -0.0
Or see this region's nucleotide sequence