Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4223 and EX28DRAFT_4224 overlap by 1 nucleotides EX28DRAFT_4224 and EX28DRAFT_4225 are separated by 2 nucleotides EX28DRAFT_4225 and EX28DRAFT_4226 are separated by 256 nucleotides
EX28DRAFT_4223: EX28DRAFT_4223 - hypothetical protein, at 29,735 to 30,949
_4223
EX28DRAFT_4224: EX28DRAFT_4224 - Predicted double-glycine peptidase, at 30,949 to 31,476
_4224
EX28DRAFT_4225: EX28DRAFT_4225 - Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain, at 31,479 to 32,210
_4225
EX28DRAFT_4226: EX28DRAFT_4226 - formate dehydrogenase alpha subunit (EC 1.2.1.2), at 32,467 to 34,614
_4226
Position (kb) 30
31
32 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 29.984 kb on - strand, within EX28DRAFT_4223 at 30.097 kb on + strand, within EX28DRAFT_4223 at 30.102 kb on + strand, within EX28DRAFT_4223 at 30.103 kb on - strand, within EX28DRAFT_4223 at 30.340 kb on - strand, within EX28DRAFT_4223 at 30.421 kb on - strand, within EX28DRAFT_4223 at 30.496 kb on + strand, within EX28DRAFT_4223 at 30.519 kb on - strand, within EX28DRAFT_4223 at 30.519 kb on - strand, within EX28DRAFT_4223 at 30.557 kb on - strand, within EX28DRAFT_4223 at 30.734 kb on - strand, within EX28DRAFT_4223 at 30.764 kb on + strand, within EX28DRAFT_4223 at 30.764 kb on + strand, within EX28DRAFT_4223 at 30.765 kb on - strand, within EX28DRAFT_4223 at 30.765 kb on - strand, within EX28DRAFT_4223 at 30.765 kb on - strand, within EX28DRAFT_4223 at 30.765 kb on - strand, within EX28DRAFT_4223 at 30.765 kb on - strand, within EX28DRAFT_4223 at 30.766 kb on + strand, within EX28DRAFT_4223 at 30.767 kb on - strand, within EX28DRAFT_4223 at 30.779 kb on - strand, within EX28DRAFT_4223 at 30.782 kb on - strand, within EX28DRAFT_4223 at 30.788 kb on + strand, within EX28DRAFT_4223 at 30.878 kb on - strand at 30.971 kb on + strand at 30.971 kb on + strand at 30.977 kb on + strand at 31.001 kb on - strand at 31.100 kb on + strand, within EX28DRAFT_4224 at 31.100 kb on + strand, within EX28DRAFT_4224 at 31.152 kb on + strand, within EX28DRAFT_4224 at 31.327 kb on + strand, within EX28DRAFT_4224 at 31.359 kb on + strand, within EX28DRAFT_4224 at 31.475 kb on + strand at 31.477 kb on + strand at 31.478 kb on - strand at 31.478 kb on - strand at 31.478 kb on - strand at 31.480 kb on + strand at 31.481 kb on - strand at 31.481 kb on - strand at 31.523 kb on - strand at 31.715 kb on - strand, within EX28DRAFT_4225 at 31.910 kb on - strand, within EX28DRAFT_4225 at 31.910 kb on - strand, within EX28DRAFT_4225 at 32.048 kb on + strand, within EX28DRAFT_4225 at 32.187 kb on + strand at 32.187 kb on + strand at 32.187 kb on + strand at 32.187 kb on + strand at 32.188 kb on - strand at 32.188 kb on - strand at 32.188 kb on - strand at 32.232 kb on + strand at 32.233 kb on - strand at 32.395 kb on - strand at 32.429 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 29,984 - EX28DRAFT_4223 0.20 -1.8 30,097 + EX28DRAFT_4223 0.30 -0.2 30,102 + EX28DRAFT_4223 0.30 -0.2 30,103 - EX28DRAFT_4223 0.30 +0.2 30,340 - EX28DRAFT_4223 0.50 -0.2 30,421 - EX28DRAFT_4223 0.56 +0.1 30,496 + EX28DRAFT_4223 0.63 -0.1 30,519 - EX28DRAFT_4223 0.65 -0.4 30,519 - EX28DRAFT_4223 0.65 -0.1 30,557 - EX28DRAFT_4223 0.68 +0.3 30,734 - EX28DRAFT_4223 0.82 +0.5 30,764 + EX28DRAFT_4223 0.85 -0.1 30,764 + EX28DRAFT_4223 0.85 +0.8 30,765 - EX28DRAFT_4223 0.85 +0.1 30,765 - EX28DRAFT_4223 0.85 -0.4 30,765 - EX28DRAFT_4223 0.85 +0.4 30,765 - EX28DRAFT_4223 0.85 -1.4 30,765 - EX28DRAFT_4223 0.85 +0.1 30,766 + EX28DRAFT_4223 0.85 -0.1 30,767 - EX28DRAFT_4223 0.85 -0.3 30,779 - EX28DRAFT_4223 0.86 -0.8 30,782 - EX28DRAFT_4223 0.86 +0.3 30,788 + EX28DRAFT_4223 0.87 +0.9 30,878 - +1.4 30,971 + -0.7 30,971 + -0.9 30,977 + -1.4 31,001 - +0.2 31,100 + EX28DRAFT_4224 0.29 -0.2 31,100 + EX28DRAFT_4224 0.29 -0.0 31,152 + EX28DRAFT_4224 0.38 -0.5 31,327 + EX28DRAFT_4224 0.72 -1.8 31,359 + EX28DRAFT_4224 0.78 -2.8 31,475 + +0.6 31,477 + -0.4 31,478 - -0.4 31,478 - +1.1 31,478 - -0.7 31,480 + +0.4 31,481 - -1.4 31,481 - +2.8 31,523 - +0.2 31,715 - EX28DRAFT_4225 0.32 +0.2 31,910 - EX28DRAFT_4225 0.59 +0.6 31,910 - EX28DRAFT_4225 0.59 -0.6 32,048 + EX28DRAFT_4225 0.78 -0.6 32,187 + -0.5 32,187 + +0.3 32,187 + +0.7 32,187 + +1.4 32,188 - -0.1 32,188 - +0.5 32,188 - +0.5 32,232 + +0.1 32,233 - +0.1 32,395 - +0.2 32,429 + +0.6
Or see this region's nucleotide sequence