Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4133 and EX28DRAFT_4134 are separated by 30 nucleotides EX28DRAFT_4134 and EX28DRAFT_4135 are separated by 206 nucleotides EX28DRAFT_4135 and EX28DRAFT_4136 are separated by 8 nucleotides EX28DRAFT_4136 and EX28DRAFT_4137 are separated by 88 nucleotides EX28DRAFT_4137 and EX28DRAFT_4138 are separated by 232 nucleotides
EX28DRAFT_4133: EX28DRAFT_4133 - ferrous iron transporter FeoB, at 111,703 to 114,021
_4133
EX28DRAFT_4134: EX28DRAFT_4134 - Fe2+ transport system protein A, at 114,052 to 114,279
_4134
EX28DRAFT_4135: EX28DRAFT_4135 - hypothetical protein, at 114,486 to 114,608
_4135
EX28DRAFT_4136: EX28DRAFT_4136 - Transcriptional accessory protein, at 114,617 to 116,947
_4136
EX28DRAFT_4137: EX28DRAFT_4137 - transcription elongation factor GreB, at 117,036 to 117,509
_4137
EX28DRAFT_4138: EX28DRAFT_4138 - Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain, at 117,742 to 118,461
_4138
Position (kb) 114
115
116
117 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 113.714 kb on + strand, within EX28DRAFT_4133 at 113.715 kb on - strand, within EX28DRAFT_4133 at 113.722 kb on - strand, within EX28DRAFT_4133 at 113.781 kb on + strand, within EX28DRAFT_4133 at 113.782 kb on - strand, within EX28DRAFT_4133 at 113.784 kb on - strand, within EX28DRAFT_4133 at 113.902 kb on - strand at 113.986 kb on - strand at 113.998 kb on + strand at 114.007 kb on - strand at 114.076 kb on + strand, within EX28DRAFT_4134 at 114.096 kb on + strand, within EX28DRAFT_4134 at 114.112 kb on + strand, within EX28DRAFT_4134 at 114.307 kb on + strand at 114.308 kb on - strand at 114.312 kb on - strand at 114.352 kb on + strand at 114.381 kb on - strand at 114.383 kb on + strand at 114.465 kb on + strand at 114.503 kb on + strand, within EX28DRAFT_4135 at 114.503 kb on + strand, within EX28DRAFT_4135 at 114.506 kb on - strand, within EX28DRAFT_4135 at 114.910 kb on + strand, within EX28DRAFT_4136 at 115.213 kb on + strand, within EX28DRAFT_4136 at 115.214 kb on - strand, within EX28DRAFT_4136 at 115.527 kb on - strand, within EX28DRAFT_4136 at 115.539 kb on + strand, within EX28DRAFT_4136 at 115.815 kb on + strand, within EX28DRAFT_4136 at 115.875 kb on + strand, within EX28DRAFT_4136 at 116.326 kb on - strand, within EX28DRAFT_4136 at 116.400 kb on + strand, within EX28DRAFT_4136 at 116.458 kb on - strand, within EX28DRAFT_4136 at 116.629 kb on - strand, within EX28DRAFT_4136 at 116.637 kb on + strand, within EX28DRAFT_4136 at 116.785 kb on + strand at 116.886 kb on - strand at 116.908 kb on + strand at 116.920 kb on + strand at 116.984 kb on + strand at 116.985 kb on - strand at 116.985 kb on - strand at 116.985 kb on - strand at 116.989 kb on - strand at 116.989 kb on - strand at 116.989 kb on - strand at 117.042 kb on + strand at 117.043 kb on - strand at 117.064 kb on + strand at 117.065 kb on - strand at 117.065 kb on - strand at 117.160 kb on + strand, within EX28DRAFT_4137 at 117.160 kb on + strand, within EX28DRAFT_4137 at 117.161 kb on - strand, within EX28DRAFT_4137 at 117.161 kb on - strand, within EX28DRAFT_4137 at 117.362 kb on - strand, within EX28DRAFT_4137 at 117.367 kb on + strand, within EX28DRAFT_4137 at 117.535 kb on + strand at 117.553 kb on + strand at 117.553 kb on + strand at 117.582 kb on + strand at 117.583 kb on - strand at 117.593 kb on + strand at 117.593 kb on + strand at 117.614 kb on - strand at 117.625 kb on - strand at 117.638 kb on - strand at 117.668 kb on + strand at 117.669 kb on - strand at 117.681 kb on - strand at 117.700 kb on - strand at 117.700 kb on - strand at 117.809 kb on - strand at 117.936 kb on + strand, within EX28DRAFT_4138
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 113,714 + EX28DRAFT_4133 0.87 +0.6 113,715 - EX28DRAFT_4133 0.87 +0.0 113,722 - EX28DRAFT_4133 0.87 +0.1 113,781 + EX28DRAFT_4133 0.90 +0.6 113,782 - EX28DRAFT_4133 0.90 +1.7 113,784 - EX28DRAFT_4133 0.90 +2.9 113,902 - +0.1 113,986 - +0.3 113,998 + +0.4 114,007 - -1.2 114,076 + EX28DRAFT_4134 0.11 +0.2 114,096 + EX28DRAFT_4134 0.19 +0.9 114,112 + EX28DRAFT_4134 0.26 +2.4 114,307 + +0.2 114,308 - +0.1 114,312 - +0.1 114,352 + -0.4 114,381 - -2.6 114,383 + -1.4 114,465 + +0.8 114,503 + EX28DRAFT_4135 0.14 +0.2 114,503 + EX28DRAFT_4135 0.14 +0.9 114,506 - EX28DRAFT_4135 0.16 -0.5 114,910 + EX28DRAFT_4136 0.13 -0.0 115,213 + EX28DRAFT_4136 0.26 +0.6 115,214 - EX28DRAFT_4136 0.26 +0.4 115,527 - EX28DRAFT_4136 0.39 +1.1 115,539 + EX28DRAFT_4136 0.40 +0.4 115,815 + EX28DRAFT_4136 0.51 +0.3 115,875 + EX28DRAFT_4136 0.54 -0.5 116,326 - EX28DRAFT_4136 0.73 -1.2 116,400 + EX28DRAFT_4136 0.76 +0.2 116,458 - EX28DRAFT_4136 0.79 -0.2 116,629 - EX28DRAFT_4136 0.86 +0.6 116,637 + EX28DRAFT_4136 0.87 -0.6 116,785 + +0.1 116,886 - +1.1 116,908 + +0.3 116,920 + +0.3 116,984 + +0.3 116,985 - +0.1 116,985 - +0.8 116,985 - +0.7 116,989 - -1.7 116,989 - -0.8 116,989 - -0.1 117,042 + +0.6 117,043 - -3.6 117,064 + +0.2 117,065 - +0.6 117,065 - +0.1 117,160 + EX28DRAFT_4137 0.26 -1.1 117,160 + EX28DRAFT_4137 0.26 -0.0 117,161 - EX28DRAFT_4137 0.26 +0.4 117,161 - EX28DRAFT_4137 0.26 +0.2 117,362 - EX28DRAFT_4137 0.69 +0.2 117,367 + EX28DRAFT_4137 0.70 +1.6 117,535 + -0.1 117,553 + +0.5 117,553 + +0.2 117,582 + -0.1 117,583 - +0.2 117,593 + +0.1 117,593 + +0.4 117,614 - -0.7 117,625 - +1.4 117,638 - +0.1 117,668 + +0.6 117,669 - +0.4 117,681 - +0.3 117,700 - -0.3 117,700 - +0.6 117,809 - +1.7 117,936 + EX28DRAFT_4138 0.27 +0.3
Or see this region's nucleotide sequence