Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3782 and EX28DRAFT_3783 are separated by 113 nucleotides EX28DRAFT_3783 and EX28DRAFT_3784 overlap by 4 nucleotides EX28DRAFT_3784 and EX28DRAFT_3785 are separated by 106 nucleotides
EX28DRAFT_3782: EX28DRAFT_3782 - prolyl-tRNA synthetase (EC 6.1.1.15), at 463,501 to 465,219
_3782
EX28DRAFT_3783: EX28DRAFT_3783 - tRNA-Thr(GGU) m(6)t(6)A37 methyltransferase TsaA, at 465,333 to 466,040
_3783
EX28DRAFT_3784: EX28DRAFT_3784 - hypothetical protein, at 466,037 to 466,441
_3784
EX28DRAFT_3785: EX28DRAFT_3785 - lipoprotein, YaeC family, at 466,548 to 467,363
_3785
Position (kb) 465
466
467 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 465.446 kb on - strand, within EX28DRAFT_3783 at 465.446 kb on - strand, within EX28DRAFT_3783 at 465.493 kb on - strand, within EX28DRAFT_3783 at 465.674 kb on + strand, within EX28DRAFT_3783 at 465.678 kb on + strand, within EX28DRAFT_3783 at 465.679 kb on - strand, within EX28DRAFT_3783 at 465.712 kb on - strand, within EX28DRAFT_3783 at 465.821 kb on + strand, within EX28DRAFT_3783 at 465.870 kb on + strand, within EX28DRAFT_3783 at 465.870 kb on + strand, within EX28DRAFT_3783 at 465.871 kb on - strand, within EX28DRAFT_3783 at 465.888 kb on + strand, within EX28DRAFT_3783 at 465.917 kb on - strand, within EX28DRAFT_3783 at 465.995 kb on - strand at 466.385 kb on + strand, within EX28DRAFT_3784 at 466.390 kb on + strand, within EX28DRAFT_3784 at 466.390 kb on + strand, within EX28DRAFT_3784 at 466.391 kb on - strand, within EX28DRAFT_3784 at 466.391 kb on - strand, within EX28DRAFT_3784 at 466.391 kb on - strand, within EX28DRAFT_3784 at 466.441 kb on + strand at 466.444 kb on + strand at 466.444 kb on + strand at 466.445 kb on - strand at 466.445 kb on - strand at 466.445 kb on - strand at 466.476 kb on - strand at 466.514 kb on - strand at 466.530 kb on + strand at 466.533 kb on + strand at 466.533 kb on + strand at 466.533 kb on + strand at 466.534 kb on - strand at 466.534 kb on - strand at 466.534 kb on - strand at 466.559 kb on + strand at 466.615 kb on + strand at 466.616 kb on - strand at 466.697 kb on + strand, within EX28DRAFT_3785 at 466.750 kb on + strand, within EX28DRAFT_3785 at 466.751 kb on - strand, within EX28DRAFT_3785 at 466.751 kb on - strand, within EX28DRAFT_3785
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 465,446 - EX28DRAFT_3783 0.16 -1.1 465,446 - EX28DRAFT_3783 0.16 +0.1 465,493 - EX28DRAFT_3783 0.23 +0.2 465,674 + EX28DRAFT_3783 0.48 +0.6 465,678 + EX28DRAFT_3783 0.49 -0.1 465,679 - EX28DRAFT_3783 0.49 +0.5 465,712 - EX28DRAFT_3783 0.54 +1.1 465,821 + EX28DRAFT_3783 0.69 +0.5 465,870 + EX28DRAFT_3783 0.76 +0.0 465,870 + EX28DRAFT_3783 0.76 -0.4 465,871 - EX28DRAFT_3783 0.76 +0.8 465,888 + EX28DRAFT_3783 0.78 -0.8 465,917 - EX28DRAFT_3783 0.82 -0.3 465,995 - +0.3 466,385 + EX28DRAFT_3784 0.86 +0.7 466,390 + EX28DRAFT_3784 0.87 -1.9 466,390 + EX28DRAFT_3784 0.87 +0.5 466,391 - EX28DRAFT_3784 0.87 +0.0 466,391 - EX28DRAFT_3784 0.87 -0.6 466,391 - EX28DRAFT_3784 0.87 +0.7 466,441 + +0.3 466,444 + +0.4 466,444 + -0.5 466,445 - -0.8 466,445 - +0.3 466,445 - -0.6 466,476 - -0.4 466,514 - +0.2 466,530 + +0.5 466,533 + -0.2 466,533 + -1.5 466,533 + +0.1 466,534 - -0.5 466,534 - -2.9 466,534 - +0.9 466,559 + +0.0 466,615 + -0.2 466,616 - -0.3 466,697 + EX28DRAFT_3785 0.18 -0.3 466,750 + EX28DRAFT_3785 0.25 +0.8 466,751 - EX28DRAFT_3785 0.25 -1.3 466,751 - EX28DRAFT_3785 0.25 +0.7
Or see this region's nucleotide sequence