Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3654 and EX28DRAFT_3655 are separated by 10 nucleotides EX28DRAFT_3655 and EX28DRAFT_3656 are separated by 337 nucleotides
EX28DRAFT_3654: EX28DRAFT_3654 - L-arabinose isomerase, at 320,023 to 321,525
_3654
EX28DRAFT_3655: EX28DRAFT_3655 - ribulokinase, at 321,536 to 323,245
_3655
EX28DRAFT_3656: EX28DRAFT_3656 - AraC-type DNA-binding domain-containing proteins, at 323,583 to 324,428
_3656
Position (kb) 321
322
323
324 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 321.018 kb on + strand, within EX28DRAFT_3654 at 321.048 kb on + strand, within EX28DRAFT_3654 at 321.282 kb on + strand, within EX28DRAFT_3654 at 321.282 kb on + strand, within EX28DRAFT_3654 at 321.283 kb on - strand, within EX28DRAFT_3654 at 321.312 kb on + strand, within EX28DRAFT_3654 at 321.321 kb on - strand, within EX28DRAFT_3654 at 321.321 kb on - strand, within EX28DRAFT_3654 at 321.321 kb on - strand, within EX28DRAFT_3654 at 321.506 kb on + strand at 321.506 kb on + strand at 321.506 kb on + strand at 321.506 kb on + strand at 321.506 kb on + strand at 321.506 kb on + strand at 321.506 kb on + strand at 321.506 kb on + strand at 321.506 kb on + strand at 321.507 kb on - strand at 321.507 kb on - strand at 321.507 kb on - strand at 321.507 kb on - strand at 321.507 kb on - strand at 321.639 kb on + strand at 322.221 kb on + strand, within EX28DRAFT_3655 at 322.222 kb on - strand, within EX28DRAFT_3655 at 322.222 kb on - strand, within EX28DRAFT_3655 at 322.226 kb on - strand, within EX28DRAFT_3655 at 322.539 kb on + strand, within EX28DRAFT_3655 at 322.539 kb on + strand, within EX28DRAFT_3655 at 322.540 kb on - strand, within EX28DRAFT_3655 at 322.794 kb on + strand, within EX28DRAFT_3655 at 322.795 kb on - strand, within EX28DRAFT_3655 at 322.818 kb on + strand, within EX28DRAFT_3655 at 323.072 kb on + strand, within EX28DRAFT_3655 at 323.072 kb on + strand, within EX28DRAFT_3655 at 323.072 kb on + strand, within EX28DRAFT_3655 at 323.073 kb on - strand, within EX28DRAFT_3655 at 323.073 kb on - strand, within EX28DRAFT_3655 at 323.074 kb on + strand, within EX28DRAFT_3655 at 323.074 kb on + strand, within EX28DRAFT_3655 at 323.074 kb on + strand, within EX28DRAFT_3655 at 323.074 kb on + strand, within EX28DRAFT_3655 at 323.075 kb on - strand at 323.076 kb on + strand at 323.165 kb on + strand at 323.183 kb on - strand at 323.401 kb on + strand at 323.477 kb on + strand at 323.478 kb on - strand at 323.521 kb on + strand at 323.532 kb on + strand at 323.716 kb on - strand, within EX28DRAFT_3656 at 323.826 kb on + strand, within EX28DRAFT_3656 at 324.041 kb on + strand, within EX28DRAFT_3656 at 324.135 kb on + strand, within EX28DRAFT_3656 at 324.135 kb on + strand, within EX28DRAFT_3656 at 324.136 kb on - strand, within EX28DRAFT_3656 at 324.136 kb on - strand, within EX28DRAFT_3656
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 321,018 + EX28DRAFT_3654 0.66 -0.3 321,048 + EX28DRAFT_3654 0.68 +1.0 321,282 + EX28DRAFT_3654 0.84 +0.5 321,282 + EX28DRAFT_3654 0.84 +0.1 321,283 - EX28DRAFT_3654 0.84 +0.4 321,312 + EX28DRAFT_3654 0.86 +0.7 321,321 - EX28DRAFT_3654 0.86 -0.4 321,321 - EX28DRAFT_3654 0.86 +0.1 321,321 - EX28DRAFT_3654 0.86 -0.7 321,506 + +0.4 321,506 + -0.7 321,506 + -0.2 321,506 + -0.7 321,506 + -3.8 321,506 + -0.4 321,506 + +0.3 321,506 + +0.2 321,506 + -0.3 321,507 - +0.2 321,507 - +0.2 321,507 - -0.1 321,507 - +0.1 321,507 - +0.5 321,639 + +0.3 322,221 + EX28DRAFT_3655 0.40 -1.1 322,222 - EX28DRAFT_3655 0.40 -0.1 322,222 - EX28DRAFT_3655 0.40 +0.4 322,226 - EX28DRAFT_3655 0.40 -0.8 322,539 + EX28DRAFT_3655 0.59 -1.5 322,539 + EX28DRAFT_3655 0.59 +0.5 322,540 - EX28DRAFT_3655 0.59 +0.6 322,794 + EX28DRAFT_3655 0.74 +0.1 322,795 - EX28DRAFT_3655 0.74 -0.1 322,818 + EX28DRAFT_3655 0.75 -0.0 323,072 + EX28DRAFT_3655 0.90 -0.0 323,072 + EX28DRAFT_3655 0.90 +0.2 323,072 + EX28DRAFT_3655 0.90 -0.0 323,073 - EX28DRAFT_3655 0.90 +0.7 323,073 - EX28DRAFT_3655 0.90 -0.1 323,074 + EX28DRAFT_3655 0.90 -0.3 323,074 + EX28DRAFT_3655 0.90 +0.2 323,074 + EX28DRAFT_3655 0.90 +0.3 323,074 + EX28DRAFT_3655 0.90 +1.3 323,075 - -0.1 323,076 + -0.6 323,165 + -0.5 323,183 - +0.1 323,401 + -1.1 323,477 + +0.4 323,478 - -0.6 323,521 + +0.2 323,532 + -1.4 323,716 - EX28DRAFT_3656 0.16 +0.1 323,826 + EX28DRAFT_3656 0.29 +0.5 324,041 + EX28DRAFT_3656 0.54 +0.3 324,135 + EX28DRAFT_3656 0.65 +0.0 324,135 + EX28DRAFT_3656 0.65 +0.1 324,136 - EX28DRAFT_3656 0.65 +1.1 324,136 - EX28DRAFT_3656 0.65 -0.2
Or see this region's nucleotide sequence