Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3181 and EX28DRAFT_3182 are separated by 59 nucleotides EX28DRAFT_3182 and EX28DRAFT_3183 are separated by 21 nucleotides EX28DRAFT_3183 and EX28DRAFT_3184 are separated by 374 nucleotides
EX28DRAFT_3180: EX28DRAFT_3180 - hypothetical protein, at 481,831 to 482,346
_3180
EX28DRAFT_3181: EX28DRAFT_3181 - RNA, at 481,922 to 482,298
_3181
EX28DRAFT_3182: EX28DRAFT_3182 - 2-hydroxy-3-oxopropionate reductase, at 482,358 to 483,248
_3182
EX28DRAFT_3183: EX28DRAFT_3183 - 2-dehydro-3-deoxyglucarate aldolase, at 483,270 to 484,040
_3183
EX28DRAFT_3184: EX28DRAFT_3184 - galactarate dehydratase, at 484,415 to 485,986
_3184
Position (kb) 483
484
485 Strain fitness (log2 ratio) -1
0
1
2
3 at 482.337 kb on + strand at 482.338 kb on - strand at 482.344 kb on + strand at 482.345 kb on - strand at 482.395 kb on + strand at 482.395 kb on + strand at 482.534 kb on + strand, within EX28DRAFT_3182 at 482.568 kb on - strand, within EX28DRAFT_3182 at 482.607 kb on - strand, within EX28DRAFT_3182 at 482.803 kb on + strand, within EX28DRAFT_3182 at 482.803 kb on + strand, within EX28DRAFT_3182 at 482.803 kb on + strand, within EX28DRAFT_3182 at 482.803 kb on + strand, within EX28DRAFT_3182 at 482.804 kb on - strand, within EX28DRAFT_3182 at 482.804 kb on - strand, within EX28DRAFT_3182 at 482.806 kb on + strand, within EX28DRAFT_3182 at 482.806 kb on + strand, within EX28DRAFT_3182 at 482.806 kb on + strand, within EX28DRAFT_3182 at 482.807 kb on - strand, within EX28DRAFT_3182 at 482.807 kb on - strand, within EX28DRAFT_3182 at 482.957 kb on + strand, within EX28DRAFT_3182 at 482.957 kb on + strand, within EX28DRAFT_3182 at 482.957 kb on + strand, within EX28DRAFT_3182 at 482.966 kb on + strand, within EX28DRAFT_3182 at 483.173 kb on - strand at 483.194 kb on + strand at 483.195 kb on - strand at 483.248 kb on + strand at 483.249 kb on - strand at 483.365 kb on - strand, within EX28DRAFT_3183 at 483.525 kb on - strand, within EX28DRAFT_3183 at 483.628 kb on + strand, within EX28DRAFT_3183 at 483.711 kb on + strand, within EX28DRAFT_3183 at 483.711 kb on + strand, within EX28DRAFT_3183 at 483.712 kb on - strand, within EX28DRAFT_3183 at 483.806 kb on + strand, within EX28DRAFT_3183 at 484.034 kb on + strand at 484.068 kb on + strand at 484.071 kb on - strand at 484.071 kb on - strand at 484.088 kb on + strand at 484.159 kb on - strand at 484.160 kb on + strand at 484.202 kb on - strand at 484.275 kb on - strand at 484.348 kb on - strand at 484.351 kb on + strand at 484.406 kb on + strand at 484.462 kb on - strand at 484.558 kb on + strand at 484.559 kb on - strand at 484.573 kb on + strand, within EX28DRAFT_3184 at 484.573 kb on + strand, within EX28DRAFT_3184 at 484.573 kb on + strand, within EX28DRAFT_3184 at 484.573 kb on + strand, within EX28DRAFT_3184 at 484.574 kb on - strand, within EX28DRAFT_3184 at 484.574 kb on - strand, within EX28DRAFT_3184 at 484.614 kb on + strand, within EX28DRAFT_3184 at 484.651 kb on + strand, within EX28DRAFT_3184 at 484.651 kb on + strand, within EX28DRAFT_3184 at 484.651 kb on + strand, within EX28DRAFT_3184 at 484.652 kb on - strand, within EX28DRAFT_3184 at 484.766 kb on + strand, within EX28DRAFT_3184 at 484.768 kb on + strand, within EX28DRAFT_3184 at 484.858 kb on - strand, within EX28DRAFT_3184 at 484.865 kb on + strand, within EX28DRAFT_3184 at 484.993 kb on + strand, within EX28DRAFT_3184 at 484.993 kb on + strand, within EX28DRAFT_3184
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 482,337 + +0.5 482,338 - +1.6 482,344 + +0.4 482,345 - +0.9 482,395 + +0.3 482,395 + +0.1 482,534 + EX28DRAFT_3182 0.20 +3.5 482,568 - EX28DRAFT_3182 0.24 -0.3 482,607 - EX28DRAFT_3182 0.28 -0.0 482,803 + EX28DRAFT_3182 0.50 +0.6 482,803 + EX28DRAFT_3182 0.50 +0.8 482,803 + EX28DRAFT_3182 0.50 -0.6 482,803 + EX28DRAFT_3182 0.50 +0.6 482,804 - EX28DRAFT_3182 0.50 +0.8 482,804 - EX28DRAFT_3182 0.50 -0.0 482,806 + EX28DRAFT_3182 0.50 +0.2 482,806 + EX28DRAFT_3182 0.50 +1.0 482,806 + EX28DRAFT_3182 0.50 +0.2 482,807 - EX28DRAFT_3182 0.50 -0.1 482,807 - EX28DRAFT_3182 0.50 +0.8 482,957 + EX28DRAFT_3182 0.67 +0.2 482,957 + EX28DRAFT_3182 0.67 -1.2 482,957 + EX28DRAFT_3182 0.67 +0.3 482,966 + EX28DRAFT_3182 0.68 -0.5 483,173 - +0.4 483,194 + -1.5 483,195 - +0.2 483,248 + +0.4 483,249 - -0.1 483,365 - EX28DRAFT_3183 0.12 +0.1 483,525 - EX28DRAFT_3183 0.33 -0.1 483,628 + EX28DRAFT_3183 0.46 -0.6 483,711 + EX28DRAFT_3183 0.57 -0.5 483,711 + EX28DRAFT_3183 0.57 -0.6 483,712 - EX28DRAFT_3183 0.57 +0.6 483,806 + EX28DRAFT_3183 0.70 -0.5 484,034 + -0.3 484,068 + +0.4 484,071 - -0.1 484,071 - +0.1 484,088 + -0.1 484,159 - -0.2 484,160 + +0.3 484,202 - -0.3 484,275 - -0.0 484,348 - -0.4 484,351 + +0.4 484,406 + +0.6 484,462 - +0.5 484,558 + +0.1 484,559 - +0.1 484,573 + EX28DRAFT_3184 0.10 -0.6 484,573 + EX28DRAFT_3184 0.10 -1.0 484,573 + EX28DRAFT_3184 0.10 -0.4 484,573 + EX28DRAFT_3184 0.10 +0.4 484,574 - EX28DRAFT_3184 0.10 +0.1 484,574 - EX28DRAFT_3184 0.10 -0.8 484,614 + EX28DRAFT_3184 0.13 +0.5 484,651 + EX28DRAFT_3184 0.15 -0.7 484,651 + EX28DRAFT_3184 0.15 +0.1 484,651 + EX28DRAFT_3184 0.15 +0.7 484,652 - EX28DRAFT_3184 0.15 +0.5 484,766 + EX28DRAFT_3184 0.22 +0.4 484,768 + EX28DRAFT_3184 0.22 +0.5 484,858 - EX28DRAFT_3184 0.28 +0.2 484,865 + EX28DRAFT_3184 0.29 -0.7 484,993 + EX28DRAFT_3184 0.37 +0.2 484,993 + EX28DRAFT_3184 0.37 +0.6
Or see this region's nucleotide sequence