Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2916 and EX28DRAFT_2917 are separated by 121 nucleotides EX28DRAFT_2917 and EX28DRAFT_2918 overlap by 6 nucleotides EX28DRAFT_2918 and EX28DRAFT_2919 are separated by 88 nucleotides
EX28DRAFT_2916: EX28DRAFT_2916 - diaminopimelate decarboxylase, at 226,401 to 227,663
_2916
EX28DRAFT_2917: EX28DRAFT_2917 - Transcriptional regulator, at 227,785 to 228,711
_2917
EX28DRAFT_2918: EX28DRAFT_2918 - aspartate racemase, at 228,706 to 229,398
_2918
EX28DRAFT_2919: EX28DRAFT_2919 - Chain length determinant protein, at 229,487 to 230,611
_2919
Position (kb) 227
228
229 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 227.072 kb on - strand, within EX28DRAFT_2916 at 227.096 kb on + strand, within EX28DRAFT_2916 at 227.131 kb on - strand, within EX28DRAFT_2916 at 227.153 kb on + strand, within EX28DRAFT_2916 at 227.154 kb on - strand, within EX28DRAFT_2916 at 227.154 kb on - strand, within EX28DRAFT_2916 at 227.288 kb on + strand, within EX28DRAFT_2916 at 227.289 kb on - strand, within EX28DRAFT_2916 at 227.292 kb on + strand, within EX28DRAFT_2916 at 227.293 kb on - strand, within EX28DRAFT_2916 at 227.484 kb on - strand, within EX28DRAFT_2916 at 227.678 kb on - strand at 227.764 kb on + strand at 227.871 kb on + strand at 227.871 kb on + strand at 227.871 kb on + strand at 228.365 kb on - strand, within EX28DRAFT_2917 at 228.391 kb on + strand, within EX28DRAFT_2917 at 228.412 kb on + strand, within EX28DRAFT_2917 at 228.413 kb on + strand, within EX28DRAFT_2917 at 228.568 kb on - strand, within EX28DRAFT_2917 at 228.686 kb on + strand at 228.867 kb on - strand, within EX28DRAFT_2918 at 228.869 kb on + strand, within EX28DRAFT_2918 at 228.919 kb on - strand, within EX28DRAFT_2918 at 229.019 kb on + strand, within EX28DRAFT_2918 at 229.020 kb on - strand, within EX28DRAFT_2918 at 229.020 kb on - strand, within EX28DRAFT_2918 at 229.089 kb on + strand, within EX28DRAFT_2918 at 229.123 kb on - strand, within EX28DRAFT_2918 at 229.146 kb on + strand, within EX28DRAFT_2918 at 229.146 kb on + strand, within EX28DRAFT_2918 at 229.152 kb on + strand, within EX28DRAFT_2918 at 229.409 kb on + strand at 229.409 kb on + strand at 229.409 kb on + strand at 229.410 kb on - strand at 229.410 kb on - strand at 229.444 kb on + strand at 229.445 kb on - strand at 229.445 kb on - strand at 229.460 kb on - strand at 229.666 kb on + strand, within EX28DRAFT_2919 at 229.705 kb on - strand, within EX28DRAFT_2919 at 229.707 kb on + strand, within EX28DRAFT_2919 at 229.707 kb on + strand, within EX28DRAFT_2919 at 229.707 kb on + strand, within EX28DRAFT_2919 at 229.707 kb on + strand, within EX28DRAFT_2919
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 227,072 - EX28DRAFT_2916 0.53 -3.1 227,096 + EX28DRAFT_2916 0.55 -3.4 227,131 - EX28DRAFT_2916 0.58 -3.9 227,153 + EX28DRAFT_2916 0.60 -2.2 227,154 - EX28DRAFT_2916 0.60 -2.0 227,154 - EX28DRAFT_2916 0.60 -3.6 227,288 + EX28DRAFT_2916 0.70 -4.2 227,289 - EX28DRAFT_2916 0.70 -3.5 227,292 + EX28DRAFT_2916 0.71 -1.0 227,293 - EX28DRAFT_2916 0.71 -5.3 227,484 - EX28DRAFT_2916 0.86 -2.8 227,678 - +0.3 227,764 + +0.0 227,871 + -3.1 227,871 + -1.8 227,871 + -2.0 228,365 - EX28DRAFT_2917 0.63 -2.9 228,391 + EX28DRAFT_2917 0.65 -3.7 228,412 + EX28DRAFT_2917 0.68 -0.9 228,413 + EX28DRAFT_2917 0.68 -3.6 228,568 - EX28DRAFT_2917 0.84 -3.6 228,686 + -0.2 228,867 - EX28DRAFT_2918 0.23 +0.0 228,869 + EX28DRAFT_2918 0.24 -0.4 228,919 - EX28DRAFT_2918 0.31 +0.3 229,019 + EX28DRAFT_2918 0.45 +0.0 229,020 - EX28DRAFT_2918 0.45 +0.1 229,020 - EX28DRAFT_2918 0.45 -1.1 229,089 + EX28DRAFT_2918 0.55 -0.1 229,123 - EX28DRAFT_2918 0.60 -0.4 229,146 + EX28DRAFT_2918 0.63 -0.9 229,146 + EX28DRAFT_2918 0.63 +0.5 229,152 + EX28DRAFT_2918 0.64 +0.0 229,409 + -0.4 229,409 + +0.7 229,409 + -0.1 229,410 - -0.5 229,410 - +0.4 229,444 + +0.8 229,445 - +1.2 229,445 - +1.3 229,460 - +0.3 229,666 + EX28DRAFT_2919 0.16 +1.3 229,705 - EX28DRAFT_2919 0.19 -0.3 229,707 + EX28DRAFT_2919 0.20 -0.4 229,707 + EX28DRAFT_2919 0.20 -1.6 229,707 + EX28DRAFT_2919 0.20 -0.1 229,707 + EX28DRAFT_2919 0.20 +0.0
Or see this region's nucleotide sequence