Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2749 and EX28DRAFT_2750 are separated by 320 nucleotides EX28DRAFT_2750 and EX28DRAFT_2751 overlap by 4 nucleotides EX28DRAFT_2751 and EX28DRAFT_2752 are separated by 179 nucleotides EX28DRAFT_2752 and EX28DRAFT_2753 are separated by 9 nucleotides
EX28DRAFT_2749: EX28DRAFT_2749 - FOG: Ankyrin repeat, at 60,389 to 60,994
_2749
EX28DRAFT_2750: EX28DRAFT_2750 - Transcriptional regulator, at 61,315 to 62,244
_2750
EX28DRAFT_2751: EX28DRAFT_2751 - Uncharacterized homolog of Blt101, at 62,241 to 62,399
_2751
EX28DRAFT_2752: EX28DRAFT_2752 - Predicted transcriptional regulators, at 62,579 to 62,878
_2752
EX28DRAFT_2753: EX28DRAFT_2753 - Rhodanese-related sulfurtransferase, at 62,888 to 63,409
_2753
Position (kb) 61
62
63 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 60.340 kb on - strand at 60.419 kb on + strand at 60.420 kb on - strand at 60.468 kb on + strand, within EX28DRAFT_2749 at 60.840 kb on + strand, within EX28DRAFT_2749 at 60.841 kb on - strand, within EX28DRAFT_2749 at 60.841 kb on - strand, within EX28DRAFT_2749 at 60.862 kb on + strand, within EX28DRAFT_2749 at 60.862 kb on + strand, within EX28DRAFT_2749 at 60.865 kb on + strand, within EX28DRAFT_2749 at 60.902 kb on - strand, within EX28DRAFT_2749 at 60.995 kb on - strand at 61.047 kb on - strand at 61.053 kb on - strand at 61.191 kb on + strand at 61.192 kb on - strand at 61.281 kb on + strand at 61.282 kb on - strand at 61.314 kb on + strand at 61.520 kb on - strand, within EX28DRAFT_2750 at 61.576 kb on - strand, within EX28DRAFT_2750 at 61.615 kb on - strand, within EX28DRAFT_2750 at 61.631 kb on - strand, within EX28DRAFT_2750 at 61.715 kb on - strand, within EX28DRAFT_2750 at 62.053 kb on + strand, within EX28DRAFT_2750 at 62.054 kb on - strand, within EX28DRAFT_2750 at 62.054 kb on - strand, within EX28DRAFT_2750 at 62.054 kb on - strand, within EX28DRAFT_2750 at 62.218 kb on + strand at 62.218 kb on + strand at 62.218 kb on + strand at 62.218 kb on + strand at 62.218 kb on + strand at 62.219 kb on - strand at 62.219 kb on - strand at 62.219 kb on - strand at 62.219 kb on - strand at 62.219 kb on - strand at 62.219 kb on - strand at 62.219 kb on - strand at 62.219 kb on - strand at 62.219 kb on - strand at 62.220 kb on + strand at 62.228 kb on + strand at 62.229 kb on - strand at 62.287 kb on + strand, within EX28DRAFT_2751 at 62.315 kb on + strand, within EX28DRAFT_2751 at 62.316 kb on - strand, within EX28DRAFT_2751 at 62.319 kb on - strand, within EX28DRAFT_2751 at 62.402 kb on - strand at 62.434 kb on - strand at 62.450 kb on + strand at 62.508 kb on - strand at 62.527 kb on - strand at 62.576 kb on - strand at 62.728 kb on - strand, within EX28DRAFT_2752 at 62.814 kb on - strand, within EX28DRAFT_2752 at 62.835 kb on + strand, within EX28DRAFT_2752 at 62.868 kb on - strand at 63.037 kb on + strand, within EX28DRAFT_2753
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 60,340 - -1.3 60,419 + -0.2 60,420 - -0.1 60,468 + EX28DRAFT_2749 0.13 -0.5 60,840 + EX28DRAFT_2749 0.74 +0.2 60,841 - EX28DRAFT_2749 0.75 -0.3 60,841 - EX28DRAFT_2749 0.75 +0.7 60,862 + EX28DRAFT_2749 0.78 -1.6 60,862 + EX28DRAFT_2749 0.78 +0.2 60,865 + EX28DRAFT_2749 0.79 -0.2 60,902 - EX28DRAFT_2749 0.85 -2.6 60,995 - -0.2 61,047 - -0.3 61,053 - +0.5 61,191 + -0.3 61,192 - +0.4 61,281 + +0.2 61,282 - -0.8 61,314 + -0.0 61,520 - EX28DRAFT_2750 0.22 +0.1 61,576 - EX28DRAFT_2750 0.28 -0.6 61,615 - EX28DRAFT_2750 0.32 +0.1 61,631 - EX28DRAFT_2750 0.34 +1.0 61,715 - EX28DRAFT_2750 0.43 +0.3 62,053 + EX28DRAFT_2750 0.79 +2.2 62,054 - EX28DRAFT_2750 0.79 +1.0 62,054 - EX28DRAFT_2750 0.79 +1.8 62,054 - EX28DRAFT_2750 0.79 +0.2 62,218 + -0.3 62,218 + +2.3 62,218 + -0.2 62,218 + +0.3 62,218 + +1.2 62,219 - +0.8 62,219 - -0.2 62,219 - +0.1 62,219 - +0.7 62,219 - -0.4 62,219 - -0.0 62,219 - -1.7 62,219 - +0.1 62,219 - +0.7 62,220 + -0.1 62,228 + -2.6 62,229 - -3.2 62,287 + EX28DRAFT_2751 0.29 +2.3 62,315 + EX28DRAFT_2751 0.47 +0.4 62,316 - EX28DRAFT_2751 0.47 +0.4 62,319 - EX28DRAFT_2751 0.49 -0.4 62,402 - -0.7 62,434 - -0.8 62,450 + +0.2 62,508 - -0.4 62,527 - +1.1 62,576 - +0.1 62,728 - EX28DRAFT_2752 0.50 -0.3 62,814 - EX28DRAFT_2752 0.78 +0.5 62,835 + EX28DRAFT_2752 0.85 -0.4 62,868 - +0.1 63,037 + EX28DRAFT_2753 0.29 +0.1
Or see this region's nucleotide sequence