Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0581 and EX28DRAFT_0582 overlap by 26 nucleotides EX28DRAFT_0582 and EX28DRAFT_0583 overlap by 4 nucleotides EX28DRAFT_0583 and EX28DRAFT_0584 are separated by 282 nucleotides
EX28DRAFT_0581: EX28DRAFT_0581 - Transcriptional regulator, at 579,769 to 580,668
_0581
EX28DRAFT_0582: EX28DRAFT_0582 - ABC-type molybdate transport system, periplasmic component, at 580,643 to 581,431
_0582
EX28DRAFT_0583: EX28DRAFT_0583 - metabolite-proton symporter, at 581,428 to 582,741
_0583
EX28DRAFT_0584: EX28DRAFT_0584 - hydro-lyases, Fe-S type, tartrate/fumarate subfamily, alpha region/hydro-lyases, Fe-S type, tartrate/fumarate subfamily, beta region, at 583,024 to 584,673
_0584
Position (kb) 581
582
583 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 580.472 kb on + strand, within EX28DRAFT_0581 at 580.473 kb on - strand, within EX28DRAFT_0581 at 580.615 kb on + strand at 580.615 kb on + strand at 580.722 kb on + strand, within EX28DRAFT_0582 at 580.795 kb on - strand, within EX28DRAFT_0582 at 580.795 kb on - strand, within EX28DRAFT_0582 at 580.822 kb on + strand, within EX28DRAFT_0582 at 580.878 kb on + strand, within EX28DRAFT_0582 at 580.878 kb on + strand, within EX28DRAFT_0582 at 580.986 kb on + strand, within EX28DRAFT_0582 at 580.987 kb on - strand, within EX28DRAFT_0582 at 581.186 kb on - strand, within EX28DRAFT_0582 at 581.298 kb on + strand, within EX28DRAFT_0582 at 581.299 kb on - strand, within EX28DRAFT_0582 at 581.354 kb on + strand at 581.354 kb on + strand at 581.355 kb on - strand at 581.411 kb on - strand at 581.419 kb on - strand at 581.431 kb on + strand at 581.431 kb on + strand at 581.458 kb on - strand at 581.458 kb on - strand at 581.489 kb on + strand at 581.489 kb on + strand at 581.624 kb on + strand, within EX28DRAFT_0583 at 581.664 kb on + strand, within EX28DRAFT_0583 at 581.665 kb on - strand, within EX28DRAFT_0583 at 581.665 kb on - strand, within EX28DRAFT_0583 at 582.243 kb on + strand, within EX28DRAFT_0583 at 582.244 kb on - strand, within EX28DRAFT_0583 at 582.244 kb on - strand, within EX28DRAFT_0583 at 582.281 kb on + strand, within EX28DRAFT_0583 at 582.416 kb on + strand, within EX28DRAFT_0583 at 582.416 kb on + strand, within EX28DRAFT_0583 at 582.506 kb on + strand, within EX28DRAFT_0583 at 582.506 kb on + strand, within EX28DRAFT_0583 at 582.506 kb on + strand, within EX28DRAFT_0583 at 582.506 kb on + strand, within EX28DRAFT_0583 at 582.507 kb on - strand, within EX28DRAFT_0583 at 582.612 kb on + strand at 582.613 kb on - strand at 582.632 kb on + strand at 582.632 kb on + strand at 582.632 kb on + strand at 582.632 kb on + strand at 582.632 kb on + strand at 582.633 kb on - strand at 582.633 kb on - strand at 582.633 kb on - strand at 582.691 kb on + strand at 582.745 kb on + strand at 582.757 kb on - strand at 582.771 kb on - strand at 582.809 kb on + strand at 582.810 kb on - strand at 582.813 kb on + strand at 582.874 kb on - strand at 582.874 kb on - strand at 582.892 kb on - strand at 582.933 kb on - strand at 582.933 kb on - strand at 583.183 kb on - strand at 583.233 kb on - strand, within EX28DRAFT_0584 at 583.233 kb on - strand, within EX28DRAFT_0584 at 583.233 kb on - strand, within EX28DRAFT_0584 at 583.324 kb on + strand, within EX28DRAFT_0584 at 583.436 kb on + strand, within EX28DRAFT_0584 at 583.638 kb on + strand, within EX28DRAFT_0584 at 583.638 kb on + strand, within EX28DRAFT_0584 at 583.639 kb on - strand, within EX28DRAFT_0584 at 583.641 kb on - strand, within EX28DRAFT_0584 at 583.641 kb on - strand, within EX28DRAFT_0584
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 580,472 + EX28DRAFT_0581 0.78 +0.5 580,473 - EX28DRAFT_0581 0.78 +0.8 580,615 + +0.3 580,615 + -0.6 580,722 + EX28DRAFT_0582 0.10 +0.2 580,795 - EX28DRAFT_0582 0.19 -0.8 580,795 - EX28DRAFT_0582 0.19 -0.7 580,822 + EX28DRAFT_0582 0.23 -1.3 580,878 + EX28DRAFT_0582 0.30 +0.4 580,878 + EX28DRAFT_0582 0.30 +0.3 580,986 + EX28DRAFT_0582 0.43 +0.9 580,987 - EX28DRAFT_0582 0.44 -0.2 581,186 - EX28DRAFT_0582 0.69 +0.2 581,298 + EX28DRAFT_0582 0.83 -0.2 581,299 - EX28DRAFT_0582 0.83 -0.7 581,354 + -3.9 581,354 + -0.3 581,355 - +0.4 581,411 - +0.1 581,419 - -0.1 581,431 + +0.4 581,431 + -0.7 581,458 - -0.9 581,458 - +0.1 581,489 + -0.8 581,489 + -1.1 581,624 + EX28DRAFT_0583 0.15 -0.4 581,664 + EX28DRAFT_0583 0.18 -1.7 581,665 - EX28DRAFT_0583 0.18 +0.4 581,665 - EX28DRAFT_0583 0.18 -1.2 582,243 + EX28DRAFT_0583 0.62 +1.0 582,244 - EX28DRAFT_0583 0.62 +0.6 582,244 - EX28DRAFT_0583 0.62 +1.1 582,281 + EX28DRAFT_0583 0.65 -3.9 582,416 + EX28DRAFT_0583 0.75 -0.3 582,416 + EX28DRAFT_0583 0.75 +0.1 582,506 + EX28DRAFT_0583 0.82 +0.1 582,506 + EX28DRAFT_0583 0.82 -0.3 582,506 + EX28DRAFT_0583 0.82 -0.3 582,506 + EX28DRAFT_0583 0.82 -1.2 582,507 - EX28DRAFT_0583 0.82 -3.0 582,612 + +0.0 582,613 - -3.6 582,632 + +0.4 582,632 + -0.4 582,632 + -0.8 582,632 + +0.6 582,632 + -0.6 582,633 - +0.2 582,633 - +0.6 582,633 - +0.5 582,691 + +0.1 582,745 + -0.5 582,757 - +0.3 582,771 - -0.0 582,809 + +0.1 582,810 - -0.1 582,813 + -0.7 582,874 - -1.1 582,874 - -1.3 582,892 - +0.2 582,933 - +0.4 582,933 - -0.3 583,183 - +0.0 583,233 - EX28DRAFT_0584 0.13 -0.3 583,233 - EX28DRAFT_0584 0.13 -0.4 583,233 - EX28DRAFT_0584 0.13 +2.8 583,324 + EX28DRAFT_0584 0.18 -0.2 583,436 + EX28DRAFT_0584 0.25 -0.7 583,638 + EX28DRAFT_0584 0.37 +0.7 583,638 + EX28DRAFT_0584 0.37 +0.2 583,639 - EX28DRAFT_0584 0.37 +0.2 583,641 - EX28DRAFT_0584 0.37 -0.1 583,641 - EX28DRAFT_0584 0.37 -0.0
Or see this region's nucleotide sequence