Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0191 and EX28DRAFT_0192 are separated by 142 nucleotides EX28DRAFT_0192 and EX28DRAFT_0193 are separated by 152 nucleotides
EX28DRAFT_0191: EX28DRAFT_0191 - The (Largely Gram-negative Bacterial) Hydrophobe/Amphiphile Efflux-1 (HAE1) Family, at 140,329 to 143,442
_0191
EX28DRAFT_0192: EX28DRAFT_0192 - Signal transduction histidine kinase, nitrate/nitrite-specific, at 143,585 to 145,279
_0192
EX28DRAFT_0193: EX28DRAFT_0193 - glutamate synthase small subunit family protein, proteobacterial, at 145,432 to 147,408
_0193
Position (kb) 143
144
145
146 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 142.939 kb on - strand, within EX28DRAFT_0191 at 142.959 kb on + strand, within EX28DRAFT_0191 at 142.959 kb on + strand, within EX28DRAFT_0191 at 142.973 kb on + strand, within EX28DRAFT_0191 at 143.213 kb on + strand at 143.213 kb on + strand at 143.213 kb on + strand at 143.213 kb on + strand at 143.213 kb on + strand at 143.214 kb on - strand at 143.214 kb on - strand at 143.214 kb on - strand at 143.214 kb on - strand at 143.214 kb on - strand at 143.474 kb on + strand at 143.475 kb on - strand at 143.475 kb on - strand at 143.493 kb on - strand at 143.544 kb on + strand at 143.544 kb on + strand at 143.544 kb on + strand at 143.581 kb on - strand at 143.581 kb on - strand at 143.586 kb on + strand at 143.587 kb on - strand at 143.667 kb on - strand at 143.698 kb on + strand at 143.713 kb on - strand at 143.805 kb on - strand, within EX28DRAFT_0192 at 144.435 kb on + strand, within EX28DRAFT_0192 at 144.436 kb on - strand, within EX28DRAFT_0192 at 144.436 kb on - strand, within EX28DRAFT_0192 at 144.449 kb on - strand, within EX28DRAFT_0192 at 144.451 kb on - strand, within EX28DRAFT_0192 at 144.451 kb on - strand, within EX28DRAFT_0192 at 144.472 kb on + strand, within EX28DRAFT_0192 at 144.610 kb on + strand, within EX28DRAFT_0192 at 144.611 kb on - strand, within EX28DRAFT_0192 at 144.694 kb on + strand, within EX28DRAFT_0192 at 144.739 kb on + strand, within EX28DRAFT_0192 at 145.022 kb on + strand, within EX28DRAFT_0192 at 145.023 kb on - strand, within EX28DRAFT_0192 at 145.150 kb on + strand at 145.151 kb on - strand at 145.226 kb on + strand at 145.228 kb on + strand at 145.229 kb on - strand at 145.324 kb on - strand at 145.377 kb on + strand at 145.377 kb on + strand at 145.377 kb on + strand at 145.381 kb on + strand at 145.382 kb on - strand at 145.393 kb on - strand at 145.393 kb on - strand at 145.443 kb on + strand at 145.444 kb on - strand at 145.671 kb on - strand, within EX28DRAFT_0193 at 145.702 kb on - strand, within EX28DRAFT_0193 at 145.885 kb on - strand, within EX28DRAFT_0193
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 142,939 - EX28DRAFT_0191 0.84 -1.2 142,959 + EX28DRAFT_0191 0.84 -0.3 142,959 + EX28DRAFT_0191 0.84 -0.2 142,973 + EX28DRAFT_0191 0.85 -0.1 143,213 + -0.1 143,213 + +0.6 143,213 + +0.6 143,213 + +0.4 143,213 + -0.0 143,214 - +0.1 143,214 - +0.6 143,214 - +0.7 143,214 - -0.2 143,214 - -0.2 143,474 + +0.4 143,475 - +0.1 143,475 - +0.3 143,493 - -0.5 143,544 + +0.2 143,544 + -0.2 143,544 + +0.3 143,581 - +0.4 143,581 - +0.1 143,586 + -0.5 143,587 - +0.4 143,667 - -0.3 143,698 + +0.0 143,713 - -1.2 143,805 - EX28DRAFT_0192 0.13 -0.4 144,435 + EX28DRAFT_0192 0.50 -2.8 144,436 - EX28DRAFT_0192 0.50 -0.3 144,436 - EX28DRAFT_0192 0.50 -0.2 144,449 - EX28DRAFT_0192 0.51 +0.0 144,451 - EX28DRAFT_0192 0.51 +0.5 144,451 - EX28DRAFT_0192 0.51 -0.4 144,472 + EX28DRAFT_0192 0.52 -0.4 144,610 + EX28DRAFT_0192 0.60 -0.7 144,611 - EX28DRAFT_0192 0.61 -0.1 144,694 + EX28DRAFT_0192 0.65 -0.2 144,739 + EX28DRAFT_0192 0.68 +0.2 145,022 + EX28DRAFT_0192 0.85 +0.2 145,023 - EX28DRAFT_0192 0.85 +0.6 145,150 + +0.3 145,151 - +1.3 145,226 + -1.5 145,228 + -0.7 145,229 - -0.5 145,324 - +0.5 145,377 + -0.4 145,377 + +0.5 145,377 + +0.3 145,381 + -1.9 145,382 - -0.4 145,393 - -0.7 145,393 - +1.1 145,443 + -0.2 145,444 - -0.2 145,671 - EX28DRAFT_0193 0.12 -1.1 145,702 - EX28DRAFT_0193 0.14 -1.6 145,885 - EX28DRAFT_0193 0.23 +1.9
Or see this region's nucleotide sequence