Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0124 and EX28DRAFT_0125 are separated by 156 nucleotides EX28DRAFT_0125 and EX28DRAFT_0126 are separated by 169 nucleotides EX28DRAFT_0126 and EX28DRAFT_0127 are separated by 293 nucleotides
EX28DRAFT_0124: EX28DRAFT_0124 - penicillin-binding protein 1C, at 66,528 to 68,852
_0124
EX28DRAFT_0125: EX28DRAFT_0125 - nucleoside diphosphate kinase (EC 2.7.4.6), at 69,009 to 69,440
_0125
EX28DRAFT_0126: EX28DRAFT_0126 - 23S rRNA (adenine(2503)-C(2))-methyltransferase, at 69,610 to 70,776
_0126
EX28DRAFT_0127: EX28DRAFT_0127 - Uncharacterized protein conserved in bacteria, at 71,070 to 72,083
_0127
Position (kb) 69
70
71 Strain fitness (log2 ratio) -2
-1
0
1 at 68.949 kb on - strand at 68.950 kb on + strand at 68.975 kb on + strand at 69.327 kb on + strand, within EX28DRAFT_0125 at 69.439 kb on - strand at 69.457 kb on - strand at 69.464 kb on - strand at 69.472 kb on - strand at 69.513 kb on + strand at 69.514 kb on - strand at 69.587 kb on + strand at 69.609 kb on + strand at 69.610 kb on - strand at 69.620 kb on + strand at 69.620 kb on + strand at 69.628 kb on - strand at 69.718 kb on - strand at 69.770 kb on - strand, within EX28DRAFT_0126 at 69.775 kb on + strand, within EX28DRAFT_0126 at 69.943 kb on + strand, within EX28DRAFT_0126 at 69.943 kb on + strand, within EX28DRAFT_0126 at 69.944 kb on - strand, within EX28DRAFT_0126 at 70.113 kb on - strand, within EX28DRAFT_0126 at 70.254 kb on + strand, within EX28DRAFT_0126 at 70.255 kb on - strand, within EX28DRAFT_0126 at 70.255 kb on - strand, within EX28DRAFT_0126 at 70.533 kb on + strand, within EX28DRAFT_0126 at 70.534 kb on - strand, within EX28DRAFT_0126 at 70.584 kb on + strand, within EX28DRAFT_0126 at 70.653 kb on + strand, within EX28DRAFT_0126 at 70.725 kb on + strand at 70.740 kb on + strand at 70.740 kb on + strand at 70.741 kb on - strand at 70.779 kb on - strand at 70.800 kb on - strand at 70.800 kb on - strand at 70.818 kb on - strand at 70.864 kb on + strand at 70.865 kb on - strand at 70.894 kb on + strand at 70.898 kb on + strand at 70.898 kb on + strand at 70.918 kb on - strand at 70.963 kb on + strand at 70.963 kb on + strand at 70.963 kb on + strand at 70.963 kb on + strand at 70.964 kb on - strand at 70.964 kb on - strand at 70.964 kb on - strand at 71.052 kb on + strand at 71.498 kb on + strand, within EX28DRAFT_0127 at 71.498 kb on + strand, within EX28DRAFT_0127 at 71.501 kb on + strand, within EX28DRAFT_0127 at 71.501 kb on + strand, within EX28DRAFT_0127 at 71.504 kb on + strand, within EX28DRAFT_0127 at 71.720 kb on + strand, within EX28DRAFT_0127 at 71.730 kb on - strand, within EX28DRAFT_0127
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 68,949 - -0.2 68,950 + +0.7 68,975 + +0.4 69,327 + EX28DRAFT_0125 0.74 -1.5 69,439 - +1.0 69,457 - -1.2 69,464 - +0.2 69,472 - -0.2 69,513 + -2.2 69,514 - -1.5 69,587 + +0.2 69,609 + +0.3 69,610 - +0.2 69,620 + -0.6 69,620 + +0.2 69,628 - +1.4 69,718 - +1.1 69,770 - EX28DRAFT_0126 0.14 -0.6 69,775 + EX28DRAFT_0126 0.14 -0.4 69,943 + EX28DRAFT_0126 0.29 -0.2 69,943 + EX28DRAFT_0126 0.29 -0.6 69,944 - EX28DRAFT_0126 0.29 +0.0 70,113 - EX28DRAFT_0126 0.43 +0.2 70,254 + EX28DRAFT_0126 0.55 -1.3 70,255 - EX28DRAFT_0126 0.55 -1.6 70,255 - EX28DRAFT_0126 0.55 -0.3 70,533 + EX28DRAFT_0126 0.79 +0.8 70,534 - EX28DRAFT_0126 0.79 -0.4 70,584 + EX28DRAFT_0126 0.83 -0.3 70,653 + EX28DRAFT_0126 0.89 +0.5 70,725 + +0.0 70,740 + +1.0 70,740 + +0.4 70,741 - -1.2 70,779 - +0.2 70,800 - -1.8 70,800 - +0.9 70,818 - +0.2 70,864 + +0.9 70,865 - +0.6 70,894 + +1.2 70,898 + -0.6 70,898 + +0.0 70,918 - -0.4 70,963 + +0.0 70,963 + +0.5 70,963 + -0.1 70,963 + +0.6 70,964 - +0.5 70,964 - -0.0 70,964 - -0.1 71,052 + +0.1 71,498 + EX28DRAFT_0127 0.42 +0.5 71,498 + EX28DRAFT_0127 0.42 +1.2 71,501 + EX28DRAFT_0127 0.43 +0.2 71,501 + EX28DRAFT_0127 0.43 +0.1 71,504 + EX28DRAFT_0127 0.43 +0.5 71,720 + EX28DRAFT_0127 0.64 -2.0 71,730 - EX28DRAFT_0127 0.65 -0.2
Or see this region's nucleotide sequence