Experiment: 30_mag_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS12930 and AMB_RS12935 are separated by 97 nucleotides AMB_RS12935 and AMB_RS12940 are separated by 5 nucleotides AMB_RS12940 and AMB_RS12945 overlap by 8 nucleotides
AMB_RS12930: AMB_RS12930 - DNA polymerase III subunit alpha, at 2,752,177 to 2,755,641
_RS12930
AMB_RS12935: AMB_RS12935 - hypothetical protein, at 2,755,739 to 2,756,119
_RS12935
AMB_RS12940: AMB_RS12940 - lipoprotein-releasing system ATP-binding protein LolD, at 2,756,125 to 2,756,805
_RS12940
AMB_RS12945: AMB_RS12945 - lipoprotein-releasing system transmembrane subunit LolC, at 2,756,798 to 2,758,042
_RS12945
Position (kb)
2755
2756
2757 Strain fitness (log2 ratio)
-2
-1
0
1 at 2755.699 kb on - strand at 2755.699 kb on - strand at 2755.888 kb on + strand, within AMB_RS12935 at 2756.091 kb on + strand at 2756.092 kb on - strand at 2756.092 kb on - strand at 2756.092 kb on - strand at 2756.094 kb on + strand at 2756.094 kb on + strand at 2756.094 kb on + strand at 2756.095 kb on - strand at 2756.095 kb on - strand at 2756.095 kb on - strand at 2756.126 kb on + strand at 2756.126 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_mag_3 remove 2,755,699 - +0.5 2,755,699 - -0.9 2,755,888 + AMB_RS12935 0.39 -0.8 2,756,091 + -1.0 2,756,092 - -0.5 2,756,092 - -0.6 2,756,092 - -1.7 2,756,094 + -0.1 2,756,094 + -0.1 2,756,094 + -1.9 2,756,095 - +0.9 2,756,095 - -0.6 2,756,095 - -1.4 2,756,126 + -1.1 2,756,126 + -0.1
Or see this region's nucleotide sequence