Experiment: 30_mag_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS12320 and AMB_RS12325 are separated by 40 nucleotides AMB_RS12325 and AMB_RS12330 are separated by 20 nucleotides AMB_RS12330 and AMB_RS12335 are separated by 87 nucleotides
AMB_RS12320: AMB_RS12320 - septal ring lytic transglycosylase RlpA family protein, at 2,628,459 to 2,629,433
_RS12320
AMB_RS12325: AMB_RS12325 - lytic murein transglycosylase, at 2,629,474 to 2,630,544
_RS12325
AMB_RS12330: AMB_RS12330 - YdcF family protein, at 2,630,565 to 2,631,365
_RS12330
AMB_RS12335: AMB_RS12335 - ABC transporter permease, at 2,631,453 to 2,632,667
_RS12335
Position (kb)
2629
2630
2631 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2628.677 kb on - strand, within AMB_RS12320 at 2628.677 kb on - strand, within AMB_RS12320 at 2629.286 kb on - strand, within AMB_RS12320 at 2629.286 kb on - strand, within AMB_RS12320 at 2629.476 kb on - strand at 2629.565 kb on + strand at 2629.565 kb on + strand at 2629.565 kb on + strand at 2629.788 kb on - strand, within AMB_RS12325 at 2629.869 kb on + strand, within AMB_RS12325 at 2629.870 kb on - strand, within AMB_RS12325 at 2629.945 kb on - strand, within AMB_RS12325 at 2630.120 kb on + strand, within AMB_RS12325 at 2630.213 kb on + strand, within AMB_RS12325 at 2630.521 kb on - strand at 2630.546 kb on + strand at 2630.547 kb on - strand at 2630.567 kb on - strand at 2630.567 kb on - strand at 2630.608 kb on + strand at 2630.608 kb on + strand at 2630.609 kb on - strand at 2630.609 kb on - strand at 2630.609 kb on - strand at 2630.719 kb on + strand, within AMB_RS12330 at 2630.834 kb on + strand, within AMB_RS12330 at 2630.834 kb on + strand, within AMB_RS12330 at 2630.834 kb on + strand, within AMB_RS12330 at 2630.834 kb on + strand, within AMB_RS12330 at 2630.835 kb on - strand, within AMB_RS12330 at 2630.835 kb on - strand, within AMB_RS12330 at 2630.835 kb on - strand, within AMB_RS12330 at 2630.835 kb on - strand, within AMB_RS12330 at 2630.835 kb on - strand, within AMB_RS12330 at 2631.372 kb on + strand at 2631.372 kb on + strand at 2631.372 kb on + strand at 2631.372 kb on + strand at 2631.372 kb on + strand at 2631.372 kb on + strand at 2631.372 kb on + strand at 2631.372 kb on + strand at 2631.372 kb on + strand at 2631.372 kb on + strand at 2631.373 kb on - strand at 2631.373 kb on - strand at 2631.373 kb on - strand at 2631.373 kb on - strand at 2631.373 kb on - strand at 2631.373 kb on - strand at 2631.373 kb on - strand at 2631.454 kb on + strand at 2631.454 kb on + strand at 2631.454 kb on + strand at 2631.455 kb on - strand at 2631.455 kb on - strand at 2631.455 kb on - strand at 2631.533 kb on - strand at 2631.533 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_mag_3 remove 2,628,677 - AMB_RS12320 0.22 -1.7 2,628,677 - AMB_RS12320 0.22 -0.3 2,629,286 - AMB_RS12320 0.85 -1.5 2,629,286 - AMB_RS12320 0.85 -3.0 2,629,476 - -0.1 2,629,565 + -0.4 2,629,565 + -1.7 2,629,565 + -1.7 2,629,788 - AMB_RS12325 0.29 +0.0 2,629,869 + AMB_RS12325 0.37 -0.3 2,629,870 - AMB_RS12325 0.37 -0.8 2,629,945 - AMB_RS12325 0.44 -1.4 2,630,120 + AMB_RS12325 0.60 -0.1 2,630,213 + AMB_RS12325 0.69 -1.1 2,630,521 - -0.2 2,630,546 + -0.6 2,630,547 - -0.4 2,630,567 - +0.4 2,630,567 - +0.9 2,630,608 + -0.2 2,630,608 + +0.4 2,630,609 - -0.6 2,630,609 - -0.3 2,630,609 - +0.4 2,630,719 + AMB_RS12330 0.19 -0.7 2,630,834 + AMB_RS12330 0.34 +0.2 2,630,834 + AMB_RS12330 0.34 +0.5 2,630,834 + AMB_RS12330 0.34 -0.8 2,630,834 + AMB_RS12330 0.34 +1.1 2,630,835 - AMB_RS12330 0.34 +0.7 2,630,835 - AMB_RS12330 0.34 -0.3 2,630,835 - AMB_RS12330 0.34 -0.2 2,630,835 - AMB_RS12330 0.34 -0.9 2,630,835 - AMB_RS12330 0.34 +0.6 2,631,372 + -0.0 2,631,372 + -0.6 2,631,372 + -1.1 2,631,372 + +0.5 2,631,372 + -0.3 2,631,372 + -0.9 2,631,372 + -0.6 2,631,372 + -0.6 2,631,372 + +0.2 2,631,372 + -0.1 2,631,373 - -2.2 2,631,373 - -1.4 2,631,373 - +1.8 2,631,373 - -0.1 2,631,373 - +0.1 2,631,373 - -0.2 2,631,373 - +0.3 2,631,454 + -0.4 2,631,454 + -0.6 2,631,454 + -0.8 2,631,455 - -0.0 2,631,455 - +0.3 2,631,455 - +1.4 2,631,533 - -0.8 2,631,533 - -0.6
Or see this region's nucleotide sequence