Experiment: 30_mag_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS12290 and AMB_RS12295 overlap by 4 nucleotides AMB_RS12295 and AMB_RS12300 are separated by 0 nucleotides AMB_RS12300 and AMB_RS12305 are separated by 32 nucleotides
AMB_RS12290: AMB_RS12290 - MBL fold metallo-hydrolase, at 2,622,412 to 2,623,185
_RS12290
AMB_RS12295: AMB_RS12295 - TatD family deoxyribonuclease, at 2,623,182 to 2,623,961
_RS12295
AMB_RS12300: AMB_RS12300 - methionine--tRNA ligase, at 2,623,962 to 2,625,512
_RS12300
AMB_RS12305: AMB_RS12305 - DNA polymerase III subunit delta', at 2,625,545 to 2,626,636
_RS12305
Position (kb)
2623
2624
2625
2626 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2622.971 kb on + strand, within AMB_RS12290 at 2622.971 kb on + strand, within AMB_RS12290 at 2623.298 kb on - strand, within AMB_RS12295 at 2623.298 kb on - strand, within AMB_RS12295 at 2623.556 kb on + strand, within AMB_RS12295 at 2623.670 kb on - strand, within AMB_RS12295 at 2623.873 kb on - strand, within AMB_RS12295 at 2623.873 kb on - strand, within AMB_RS12295 at 2623.985 kb on - strand at 2623.985 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_mag_3 remove 2,622,971 + AMB_RS12290 0.72 -1.0 2,622,971 + AMB_RS12290 0.72 -0.9 2,623,298 - AMB_RS12295 0.15 -0.1 2,623,298 - AMB_RS12295 0.15 -2.9 2,623,556 + AMB_RS12295 0.48 -2.0 2,623,670 - AMB_RS12295 0.63 +1.1 2,623,873 - AMB_RS12295 0.89 -2.7 2,623,873 - AMB_RS12295 0.89 +1.3 2,623,985 - -0.4 2,623,985 - +1.6
Or see this region's nucleotide sequence