Experiment: 30_mag_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS10590 and AMB_RS10600 are separated by 1 nucleotides AMB_RS10600 and AMB_RS10605 are separated by 109 nucleotides AMB_RS10605 and AMB_RS10610 overlap by 1 nucleotides AMB_RS10610 and AMB_RS10615 are separated by 155 nucleotides
AMB_RS10590: AMB_RS10590 - 50S ribosomal protein L9, at 2,274,409 to 2,274,972
_RS10590
AMB_RS10600: AMB_RS10600 - hypothetical protein, at 2,274,974 to 2,275,839
_RS10600
AMB_RS10605: AMB_RS10605 - 30S ribosomal protein S18, at 2,275,949 to 2,276,227
_RS10605
AMB_RS10610: AMB_RS10610 - 30S ribosomal protein S6, at 2,276,227 to 2,276,661
_RS10610
AMB_RS10615: AMB_RS10615 - HAMP domain-containing protein, at 2,276,817 to 2,278,982
_RS10615
Position (kb)
2275
2276
2277 Strain fitness (log2 ratio)
-1
0
1 at 2274.976 kb on - strand at 2274.976 kb on - strand at 2275.092 kb on + strand, within AMB_RS10600 at 2275.093 kb on - strand, within AMB_RS10600 at 2275.093 kb on - strand, within AMB_RS10600 at 2275.093 kb on - strand, within AMB_RS10600 at 2275.093 kb on - strand, within AMB_RS10600 at 2275.107 kb on + strand, within AMB_RS10600 at 2275.210 kb on - strand, within AMB_RS10600 at 2275.210 kb on - strand, within AMB_RS10600 at 2276.720 kb on - strand at 2276.720 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_mag_3 remove 2,274,976 - -0.3 2,274,976 - -0.4 2,275,092 + AMB_RS10600 0.14 -0.7 2,275,093 - AMB_RS10600 0.14 -1.4 2,275,093 - AMB_RS10600 0.14 -0.6 2,275,093 - AMB_RS10600 0.14 +1.3 2,275,093 - AMB_RS10600 0.14 +1.0 2,275,107 + AMB_RS10600 0.15 +0.3 2,275,210 - AMB_RS10600 0.27 -0.6 2,275,210 - AMB_RS10600 0.27 -0.7 2,276,720 - -0.2 2,276,720 - -1.7
Or see this region's nucleotide sequence