Experiment: 30_mag_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS04600 and AMB_RS04605 are separated by 825 nucleotides AMB_RS04605 and AMB_RS04610 are separated by 98 nucleotides AMB_RS04610 and AMB_RS04615 are separated by 45 nucleotides AMB_RS04615 and AMB_RS04620 are separated by 243 nucleotides
AMB_RS04600: AMB_RS04600 - membrane protein, at 962,681 to 964,069
_RS04600
AMB_RS04605: AMB_RS04605 - 16S ribosomal RNA, at 964,895 to 966,392
_RS04605
AMB_RS04610: AMB_RS04610 - tRNA-Ile, at 966,491 to 966,567
_RS04610
AMB_RS04615: AMB_RS04615 - tRNA-Ala, at 966,613 to 966,688
_RS04615
AMB_RS04620: AMB_RS04620 - 23S ribosomal RNA, at 966,932 to 969,685
_RS04620
Position (kb)
964
965
966
967 Strain fitness (log2 ratio)
-2
-1
0
1 at 964.088 kb on - strand at 964.088 kb on - strand at 964.091 kb on - strand at 964.091 kb on - strand at 964.091 kb on - strand at 964.091 kb on - strand at 964.091 kb on - strand at 964.091 kb on - strand at 964.153 kb on - strand at 964.153 kb on - strand at 964.155 kb on - strand at 964.155 kb on - strand at 964.225 kb on + strand at 964.225 kb on + strand at 964.226 kb on - strand at 964.226 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_mag_3 remove 964,088 - -0.7 964,088 - +0.0 964,091 - +0.4 964,091 - +0.2 964,091 - -1.8 964,091 - +0.3 964,091 - -0.7 964,091 - -0.5 964,153 - +0.1 964,153 - -0.4 964,155 - -0.3 964,155 - -0.6 964,225 + +0.3 964,225 + +1.4 964,226 - +1.0 964,226 - -0.4
Or see this region's nucleotide sequence