Experiment: 30_mag_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS04015 and AMB_RS04020 are separated by 102 nucleotides AMB_RS04020 and AMB_RS04025 are separated by 120 nucleotides AMB_RS04025 and AMB_RS04030 are separated by 2 nucleotides
AMB_RS04015: AMB_RS04015 - phosphoenolpyruvate carboxykinase (GTP), at 841,033 to 842,886
_RS04015
AMB_RS04020: AMB_RS04020 - hypothetical protein, at 842,989 to 843,423
_RS04020
AMB_RS04025: AMB_RS04025 - glutathione S-transferase, at 843,544 to 844,149
_RS04025
AMB_RS04030: AMB_RS04030 - cupin, at 844,152 to 844,577
_RS04030
Position (kb) 842
843
844 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 842.952 kb on + strand at 842.952 kb on + strand at 842.953 kb on - strand at 842.953 kb on - strand at 842.953 kb on - strand at 842.953 kb on - strand at 842.953 kb on - strand at 842.958 kb on + strand at 842.991 kb on - strand at 843.215 kb on + strand, within AMB_RS04020 at 843.215 kb on + strand, within AMB_RS04020 at 843.215 kb on + strand, within AMB_RS04020 at 843.215 kb on + strand, within AMB_RS04020 at 843.215 kb on + strand, within AMB_RS04020 at 843.215 kb on + strand, within AMB_RS04020 at 843.215 kb on + strand, within AMB_RS04020 at 843.215 kb on + strand, within AMB_RS04020 at 843.216 kb on - strand, within AMB_RS04020 at 843.216 kb on - strand, within AMB_RS04020 at 843.216 kb on - strand, within AMB_RS04020 at 843.216 kb on - strand, within AMB_RS04020 at 843.216 kb on - strand, within AMB_RS04020 at 843.216 kb on - strand, within AMB_RS04020 at 843.216 kb on - strand, within AMB_RS04020 at 843.216 kb on - strand, within AMB_RS04020 at 843.418 kb on + strand at 843.419 kb on - strand at 843.419 kb on - strand at 843.465 kb on + strand at 843.465 kb on + strand at 843.465 kb on + strand at 843.465 kb on + strand at 843.465 kb on + strand at 843.465 kb on + strand at 843.465 kb on + strand at 843.465 kb on + strand at 843.466 kb on - strand at 843.466 kb on - strand at 843.466 kb on - strand at 843.466 kb on - strand at 843.466 kb on - strand at 843.556 kb on + strand at 843.557 kb on - strand at 843.575 kb on - strand at 843.575 kb on - strand at 843.755 kb on - strand, within AMB_RS04025 at 844.033 kb on + strand, within AMB_RS04025 at 844.033 kb on + strand, within AMB_RS04025 at 844.033 kb on + strand, within AMB_RS04025 at 844.034 kb on - strand, within AMB_RS04025 at 844.034 kb on - strand, within AMB_RS04025 at 844.275 kb on + strand, within AMB_RS04030 at 844.275 kb on + strand, within AMB_RS04030 at 844.275 kb on + strand, within AMB_RS04030 at 844.275 kb on + strand, within AMB_RS04030 at 844.275 kb on + strand, within AMB_RS04030 at 844.275 kb on + strand, within AMB_RS04030 at 844.275 kb on + strand, within AMB_RS04030 at 844.276 kb on - strand, within AMB_RS04030 at 844.276 kb on - strand, within AMB_RS04030 at 844.276 kb on - strand, within AMB_RS04030 at 844.276 kb on - strand, within AMB_RS04030 at 844.276 kb on - strand, within AMB_RS04030 at 844.276 kb on - strand, within AMB_RS04030 at 844.276 kb on - strand, within AMB_RS04030 at 844.278 kb on + strand, within AMB_RS04030 at 844.278 kb on + strand, within AMB_RS04030 at 844.279 kb on - strand, within AMB_RS04030 at 844.333 kb on + strand, within AMB_RS04030 at 844.333 kb on + strand, within AMB_RS04030 at 844.333 kb on + strand, within AMB_RS04030 at 844.333 kb on + strand, within AMB_RS04030 at 844.341 kb on + strand, within AMB_RS04030 at 844.341 kb on + strand, within AMB_RS04030 at 844.342 kb on - strand, within AMB_RS04030 at 844.342 kb on - strand, within AMB_RS04030 at 844.342 kb on - strand, within AMB_RS04030 at 844.342 kb on - strand, within AMB_RS04030
Per-strain Table
Position Strand Gene LocusTag Fraction 30_mag_3 remove 842,952 + -0.7 842,952 + -0.0 842,953 - -0.7 842,953 - +1.8 842,953 - +0.2 842,953 - -0.2 842,953 - +0.8 842,958 + -1.0 842,991 - -0.2 843,215 + AMB_RS04020 0.52 -0.4 843,215 + AMB_RS04020 0.52 -0.2 843,215 + AMB_RS04020 0.52 +0.0 843,215 + AMB_RS04020 0.52 -0.2 843,215 + AMB_RS04020 0.52 +0.6 843,215 + AMB_RS04020 0.52 +0.6 843,215 + AMB_RS04020 0.52 -0.8 843,215 + AMB_RS04020 0.52 -0.7 843,216 - AMB_RS04020 0.52 +0.2 843,216 - AMB_RS04020 0.52 -0.2 843,216 - AMB_RS04020 0.52 -0.9 843,216 - AMB_RS04020 0.52 -0.7 843,216 - AMB_RS04020 0.52 -0.2 843,216 - AMB_RS04020 0.52 -0.9 843,216 - AMB_RS04020 0.52 -0.8 843,216 - AMB_RS04020 0.52 -0.3 843,418 + -1.3 843,419 - +1.1 843,419 - +0.5 843,465 + -0.3 843,465 + -0.3 843,465 + -0.4 843,465 + +0.5 843,465 + +0.0 843,465 + +0.3 843,465 + -0.6 843,465 + +0.6 843,466 - -0.4 843,466 - -0.5 843,466 - +0.4 843,466 - -0.1 843,466 - -0.8 843,556 + -0.4 843,557 - -0.2 843,575 - -0.4 843,575 - -0.1 843,755 - AMB_RS04025 0.35 -0.1 844,033 + AMB_RS04025 0.81 -1.3 844,033 + AMB_RS04025 0.81 -0.6 844,033 + AMB_RS04025 0.81 -1.0 844,034 - AMB_RS04025 0.81 -1.3 844,034 - AMB_RS04025 0.81 -0.8 844,275 + AMB_RS04030 0.29 -0.1 844,275 + AMB_RS04030 0.29 -1.2 844,275 + AMB_RS04030 0.29 -0.2 844,275 + AMB_RS04030 0.29 -0.2 844,275 + AMB_RS04030 0.29 +0.8 844,275 + AMB_RS04030 0.29 -0.9 844,275 + AMB_RS04030 0.29 -1.8 844,276 - AMB_RS04030 0.29 -0.9 844,276 - AMB_RS04030 0.29 -0.9 844,276 - AMB_RS04030 0.29 -0.6 844,276 - AMB_RS04030 0.29 -0.9 844,276 - AMB_RS04030 0.29 -3.7 844,276 - AMB_RS04030 0.29 -0.2 844,276 - AMB_RS04030 0.29 +2.6 844,278 + AMB_RS04030 0.30 -0.5 844,278 + AMB_RS04030 0.30 -0.4 844,279 - AMB_RS04030 0.30 -0.5 844,333 + AMB_RS04030 0.42 -0.6 844,333 + AMB_RS04030 0.42 +1.0 844,333 + AMB_RS04030 0.42 +0.7 844,333 + AMB_RS04030 0.42 -0.2 844,341 + AMB_RS04030 0.44 +1.0 844,341 + AMB_RS04030 0.44 +0.0 844,342 - AMB_RS04030 0.45 -1.4 844,342 - AMB_RS04030 0.45 -1.1 844,342 - AMB_RS04030 0.45 -0.7 844,342 - AMB_RS04030 0.45 -0.4
Or see this region's nucleotide sequence