Experiment: 30_mag_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS03140 and AMB_RS03145 are separated by 355 nucleotides AMB_RS03145 and AMB_RS03150 overlap by 4 nucleotides AMB_RS03150 and AMB_RS03155 are separated by 2 nucleotides AMB_RS03155 and AMB_RS03160 overlap by 4 nucleotides
AMB_RS03140: AMB_RS03140 - isocitrate dehydrogenase (NADP(+)), at 656,080 to 657,300
_RS03140
AMB_RS03145: AMB_RS03145 - flagellar biosynthetic protein FliP, at 657,656 to 658,408
_RS03145
AMB_RS03150: AMB_RS03150 - hypothetical protein, at 658,405 to 658,737
_RS03150
AMB_RS03155: AMB_RS03155 - hypothetical protein, at 658,740 to 659,006
_RS03155
AMB_RS03160: AMB_RS03160 - flagellar protein FhlB, at 659,003 to 659,356
_RS03160
Position (kb)
657
658
659 Strain fitness (log2 ratio)
-2
-1
0
1 at 657.541 kb on - strand at 657.580 kb on - strand at 657.598 kb on + strand at 657.599 kb on - strand at 657.664 kb on - strand at 657.664 kb on - strand at 657.684 kb on - strand at 657.684 kb on - strand at 657.704 kb on + strand at 657.705 kb on - strand at 657.705 kb on - strand at 657.705 kb on - strand at 657.705 kb on - strand at 657.709 kb on + strand at 657.710 kb on - strand at 658.133 kb on - strand, within AMB_RS03145 at 658.721 kb on + strand at 658.721 kb on + strand at 658.721 kb on + strand at 658.721 kb on + strand at 658.722 kb on - strand at 658.722 kb on - strand at 658.722 kb on - strand at 658.722 kb on - strand at 659.055 kb on + strand, within AMB_RS03160 at 659.061 kb on + strand, within AMB_RS03160 at 659.061 kb on + strand, within AMB_RS03160 at 659.061 kb on + strand, within AMB_RS03160 at 659.061 kb on + strand, within AMB_RS03160 at 659.061 kb on + strand, within AMB_RS03160 at 659.061 kb on + strand, within AMB_RS03160 at 659.061 kb on + strand, within AMB_RS03160 at 659.061 kb on + strand, within AMB_RS03160 at 659.061 kb on + strand, within AMB_RS03160 at 659.061 kb on + strand, within AMB_RS03160 at 659.062 kb on - strand, within AMB_RS03160 at 659.062 kb on - strand, within AMB_RS03160 at 659.062 kb on - strand, within AMB_RS03160 at 659.062 kb on - strand, within AMB_RS03160 at 659.062 kb on - strand, within AMB_RS03160 at 659.062 kb on - strand, within AMB_RS03160 at 659.062 kb on - strand, within AMB_RS03160 at 659.062 kb on - strand, within AMB_RS03160
Per-strain Table
Position Strand Gene LocusTag Fraction 30_mag_3 remove 657,541 - -0.3 657,580 - -1.0 657,598 + -0.6 657,599 - -0.2 657,664 - -0.4 657,664 - +0.9 657,684 - -0.2 657,684 - +0.1 657,704 + +0.0 657,705 - -0.6 657,705 - -0.6 657,705 - -0.3 657,705 - -0.2 657,709 + -0.0 657,710 - +0.3 658,133 - AMB_RS03145 0.63 +0.7 658,721 + +1.5 658,721 + -0.1 658,721 + -0.0 658,721 + -0.8 658,722 - -0.5 658,722 - -0.7 658,722 - +0.1 658,722 - -1.1 659,055 + AMB_RS03160 0.15 -0.2 659,061 + AMB_RS03160 0.16 -0.5 659,061 + AMB_RS03160 0.16 -0.3 659,061 + AMB_RS03160 0.16 -0.4 659,061 + AMB_RS03160 0.16 -1.9 659,061 + AMB_RS03160 0.16 -0.5 659,061 + AMB_RS03160 0.16 -0.3 659,061 + AMB_RS03160 0.16 +0.2 659,061 + AMB_RS03160 0.16 -0.3 659,061 + AMB_RS03160 0.16 +0.3 659,061 + AMB_RS03160 0.16 -0.2 659,062 - AMB_RS03160 0.17 -0.7 659,062 - AMB_RS03160 0.17 -0.2 659,062 - AMB_RS03160 0.17 +0.2 659,062 - AMB_RS03160 0.17 -0.6 659,062 - AMB_RS03160 0.17 -0.6 659,062 - AMB_RS03160 0.17 +0.3 659,062 - AMB_RS03160 0.17 +1.6 659,062 - AMB_RS03160 0.17 -0.8
Or see this region's nucleotide sequence