Experiment: 30_mag_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS01195 and AMB_RS01200 are separated by 106 nucleotides AMB_RS01200 and AMB_RS01205 are separated by 3 nucleotides AMB_RS01205 and AMB_RS01210 are separated by 17 nucleotides AMB_RS01210 and AMB_RS01215 are separated by 147 nucleotides
AMB_RS01195: AMB_RS01195 - DUF4237 domain-containing protein, at 266,343 to 266,819
_RS01195
AMB_RS01200: AMB_RS01200 - DUF4237 domain-containing protein, at 266,926 to 267,396
_RS01200
AMB_RS01205: AMB_RS01205 - nucleoside deaminase, at 267,400 to 267,828
_RS01205
AMB_RS01210: AMB_RS01210 - FCD domain-containing protein, at 267,846 to 268,622
_RS01210
AMB_RS01215: AMB_RS01215 - pyruvate carboxylase, at 268,770 to 272,234
_RS01215
Position (kb) 267
268 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 266.628 kb on + strand, within AMB_RS01195 at 266.629 kb on - strand, within AMB_RS01195 at 266.629 kb on - strand, within AMB_RS01195 at 266.649 kb on + strand, within AMB_RS01195 at 266.649 kb on + strand, within AMB_RS01195 at 266.649 kb on + strand, within AMB_RS01195 at 266.650 kb on + strand, within AMB_RS01195 at 266.651 kb on + strand, within AMB_RS01195 at 266.651 kb on + strand, within AMB_RS01195 at 266.652 kb on - strand, within AMB_RS01195 at 266.658 kb on + strand, within AMB_RS01195 at 266.741 kb on + strand, within AMB_RS01195 at 266.741 kb on + strand, within AMB_RS01195 at 267.054 kb on + strand, within AMB_RS01200 at 267.054 kb on + strand, within AMB_RS01200 at 267.055 kb on - strand, within AMB_RS01200 at 267.055 kb on - strand, within AMB_RS01200 at 267.055 kb on - strand, within AMB_RS01200 at 267.055 kb on - strand, within AMB_RS01200 at 267.157 kb on + strand, within AMB_RS01200 at 267.157 kb on + strand, within AMB_RS01200 at 267.157 kb on + strand, within AMB_RS01200 at 267.157 kb on + strand, within AMB_RS01200 at 267.157 kb on + strand, within AMB_RS01200 at 267.158 kb on - strand, within AMB_RS01200 at 267.202 kb on + strand, within AMB_RS01200 at 267.202 kb on + strand, within AMB_RS01200 at 267.202 kb on + strand, within AMB_RS01200 at 267.202 kb on + strand, within AMB_RS01200 at 267.202 kb on + strand, within AMB_RS01200 at 267.202 kb on + strand, within AMB_RS01200 at 267.203 kb on - strand, within AMB_RS01200 at 267.203 kb on - strand, within AMB_RS01200 at 267.203 kb on - strand, within AMB_RS01200 at 267.203 kb on - strand, within AMB_RS01200 at 267.203 kb on - strand, within AMB_RS01200 at 267.203 kb on - strand, within AMB_RS01200 at 267.223 kb on + strand, within AMB_RS01200 at 267.223 kb on + strand, within AMB_RS01200 at 267.223 kb on + strand, within AMB_RS01200 at 267.226 kb on + strand, within AMB_RS01200 at 267.226 kb on + strand, within AMB_RS01200 at 267.226 kb on + strand, within AMB_RS01200 at 267.226 kb on + strand, within AMB_RS01200 at 267.226 kb on + strand, within AMB_RS01200 at 267.226 kb on + strand, within AMB_RS01200 at 267.227 kb on - strand, within AMB_RS01200 at 267.227 kb on - strand, within AMB_RS01200 at 267.227 kb on - strand, within AMB_RS01200 at 267.227 kb on - strand, within AMB_RS01200 at 267.233 kb on - strand, within AMB_RS01200 at 267.233 kb on - strand, within AMB_RS01200 at 267.233 kb on - strand, within AMB_RS01200 at 267.233 kb on - strand, within AMB_RS01200 at 268.079 kb on - strand, within AMB_RS01210 at 268.639 kb on - strand at 268.652 kb on - strand at 268.672 kb on + strand at 268.673 kb on - strand at 268.694 kb on + strand at 268.713 kb on + strand at 268.714 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_mag_3 remove 266,628 + AMB_RS01195 0.60 -1.0 266,629 - AMB_RS01195 0.60 +0.0 266,629 - AMB_RS01195 0.60 -0.6 266,649 + AMB_RS01195 0.64 -0.2 266,649 + AMB_RS01195 0.64 +0.1 266,649 + AMB_RS01195 0.64 +0.1 266,650 + AMB_RS01195 0.64 +1.3 266,651 + AMB_RS01195 0.65 -0.4 266,651 + AMB_RS01195 0.65 -0.3 266,652 - AMB_RS01195 0.65 -0.4 266,658 + AMB_RS01195 0.66 +0.4 266,741 + AMB_RS01195 0.83 -0.2 266,741 + AMB_RS01195 0.83 -0.8 267,054 + AMB_RS01200 0.27 +0.3 267,054 + AMB_RS01200 0.27 -0.5 267,055 - AMB_RS01200 0.27 -0.2 267,055 - AMB_RS01200 0.27 -0.4 267,055 - AMB_RS01200 0.27 -0.8 267,055 - AMB_RS01200 0.27 -2.3 267,157 + AMB_RS01200 0.49 -1.1 267,157 + AMB_RS01200 0.49 +0.2 267,157 + AMB_RS01200 0.49 +0.4 267,157 + AMB_RS01200 0.49 +0.3 267,157 + AMB_RS01200 0.49 +0.0 267,158 - AMB_RS01200 0.49 -0.1 267,202 + AMB_RS01200 0.59 +0.2 267,202 + AMB_RS01200 0.59 -0.5 267,202 + AMB_RS01200 0.59 -0.6 267,202 + AMB_RS01200 0.59 -0.3 267,202 + AMB_RS01200 0.59 -4.0 267,202 + AMB_RS01200 0.59 -0.4 267,203 - AMB_RS01200 0.59 -0.3 267,203 - AMB_RS01200 0.59 -0.2 267,203 - AMB_RS01200 0.59 -1.2 267,203 - AMB_RS01200 0.59 -0.5 267,203 - AMB_RS01200 0.59 -1.7 267,203 - AMB_RS01200 0.59 +0.4 267,223 + AMB_RS01200 0.63 +0.4 267,223 + AMB_RS01200 0.63 +1.9 267,223 + AMB_RS01200 0.63 -0.2 267,226 + AMB_RS01200 0.64 -0.5 267,226 + AMB_RS01200 0.64 -0.7 267,226 + AMB_RS01200 0.64 -0.4 267,226 + AMB_RS01200 0.64 +0.1 267,226 + AMB_RS01200 0.64 -0.7 267,226 + AMB_RS01200 0.64 -0.4 267,227 - AMB_RS01200 0.64 -1.9 267,227 - AMB_RS01200 0.64 -0.1 267,227 - AMB_RS01200 0.64 -0.7 267,227 - AMB_RS01200 0.64 -1.7 267,233 - AMB_RS01200 0.65 -0.5 267,233 - AMB_RS01200 0.65 -0.2 267,233 - AMB_RS01200 0.65 -1.3 267,233 - AMB_RS01200 0.65 +0.2 268,079 - AMB_RS01210 0.30 +0.3 268,639 - -0.5 268,652 - -2.4 268,672 + +0.3 268,673 - -0.8 268,694 + +0.1 268,713 + +1.0 268,714 - -0.4
Or see this region's nucleotide sequence