Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4495 and EX28DRAFT_4496 are separated by 255 nucleotides EX28DRAFT_4496 and EX28DRAFT_4497 are separated by 139 nucleotides
EX28DRAFT_4495: EX28DRAFT_4495 - Dienelactone hydrolase and related enzymes, at 62,121 to 62,927
_4495
EX28DRAFT_4496: EX28DRAFT_4496 - uridine phosphorylase, at 63,183 to 63,944
_4496
EX28DRAFT_4497: EX28DRAFT_4497 - Uncharacterized protein conserved in bacteria, at 64,084 to 65,541
_4497
Position (kb)
63
64 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 62.198 kb on - strand at 62.198 kb on - strand at 62.467 kb on + strand, within EX28DRAFT_4495 at 62.477 kb on + strand, within EX28DRAFT_4495 at 62.477 kb on + strand, within EX28DRAFT_4495 at 62.477 kb on + strand, within EX28DRAFT_4495 at 62.478 kb on - strand, within EX28DRAFT_4495 at 62.659 kb on + strand, within EX28DRAFT_4495 at 62.740 kb on - strand, within EX28DRAFT_4495 at 62.962 kb on - strand at 62.962 kb on - strand at 62.962 kb on - strand at 62.965 kb on + strand at 62.965 kb on + strand at 62.966 kb on - strand at 62.966 kb on - strand at 62.967 kb on + strand at 63.031 kb on - strand at 63.032 kb on + strand at 63.032 kb on + strand at 63.033 kb on - strand at 63.122 kb on - strand at 63.182 kb on + strand at 63.182 kb on + strand at 63.182 kb on + strand at 63.182 kb on + strand at 63.182 kb on + strand at 63.183 kb on - strand at 63.183 kb on - strand at 63.183 kb on - strand at 63.183 kb on - strand at 63.183 kb on - strand at 63.188 kb on + strand at 63.300 kb on - strand, within EX28DRAFT_4496 at 63.443 kb on + strand, within EX28DRAFT_4496 at 63.712 kb on + strand, within EX28DRAFT_4496 at 63.713 kb on - strand, within EX28DRAFT_4496 at 63.713 kb on - strand, within EX28DRAFT_4496 at 63.765 kb on + strand, within EX28DRAFT_4496 at 63.852 kb on - strand, within EX28DRAFT_4496 at 63.942 kb on + strand at 63.942 kb on + strand at 63.943 kb on - strand at 64.063 kb on + strand at 64.063 kb on + strand at 64.064 kb on - strand at 64.064 kb on - strand at 64.089 kb on + strand at 64.090 kb on - strand at 64.305 kb on + strand, within EX28DRAFT_4497 at 64.311 kb on + strand, within EX28DRAFT_4497 at 64.311 kb on + strand, within EX28DRAFT_4497 at 64.312 kb on - strand, within EX28DRAFT_4497 at 64.312 kb on - strand, within EX28DRAFT_4497 at 64.312 kb on - strand, within EX28DRAFT_4497 at 64.312 kb on - strand, within EX28DRAFT_4497 at 64.756 kb on + strand, within EX28DRAFT_4497 at 64.757 kb on - strand, within EX28DRAFT_4497 at 64.873 kb on + strand, within EX28DRAFT_4497 at 64.874 kb on - strand, within EX28DRAFT_4497 at 64.874 kb on - strand, within EX28DRAFT_4497 at 64.882 kb on + strand, within EX28DRAFT_4497 at 64.882 kb on + strand, within EX28DRAFT_4497 at 64.882 kb on + strand, within EX28DRAFT_4497 at 64.903 kb on + strand, within EX28DRAFT_4497 at 64.904 kb on - strand, within EX28DRAFT_4497
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 62,198 - +1.9 62,198 - +0.7 62,467 + EX28DRAFT_4495 0.43 +0.4 62,477 + EX28DRAFT_4495 0.44 +0.2 62,477 + EX28DRAFT_4495 0.44 +0.7 62,477 + EX28DRAFT_4495 0.44 +0.4 62,478 - EX28DRAFT_4495 0.44 -0.3 62,659 + EX28DRAFT_4495 0.67 -0.1 62,740 - EX28DRAFT_4495 0.77 +0.6 62,962 - +0.4 62,962 - -0.4 62,962 - -0.6 62,965 + -0.3 62,965 + -0.3 62,966 - +1.8 62,966 - +0.1 62,967 + +0.0 63,031 - -2.7 63,032 + -0.3 63,032 + -0.0 63,033 - +0.7 63,122 - +0.3 63,182 + -0.1 63,182 + -0.4 63,182 + -0.5 63,182 + -0.1 63,182 + +0.8 63,183 - +0.2 63,183 - +0.7 63,183 - -0.2 63,183 - -0.4 63,183 - +1.2 63,188 + +1.2 63,300 - EX28DRAFT_4496 0.15 -1.6 63,443 + EX28DRAFT_4496 0.34 -1.1 63,712 + EX28DRAFT_4496 0.69 -3.3 63,713 - EX28DRAFT_4496 0.70 -0.2 63,713 - EX28DRAFT_4496 0.70 -0.6 63,765 + EX28DRAFT_4496 0.76 -0.4 63,852 - EX28DRAFT_4496 0.88 -1.2 63,942 + +0.4 63,942 + -1.8 63,943 - -1.6 64,063 + -1.8 64,063 + +0.1 64,064 - +0.3 64,064 - -1.3 64,089 + +0.7 64,090 - -0.3 64,305 + EX28DRAFT_4497 0.15 -1.1 64,311 + EX28DRAFT_4497 0.16 +0.7 64,311 + EX28DRAFT_4497 0.16 -1.7 64,312 - EX28DRAFT_4497 0.16 +0.5 64,312 - EX28DRAFT_4497 0.16 +1.0 64,312 - EX28DRAFT_4497 0.16 -0.1 64,312 - EX28DRAFT_4497 0.16 -1.5 64,756 + EX28DRAFT_4497 0.46 -0.3 64,757 - EX28DRAFT_4497 0.46 +0.2 64,873 + EX28DRAFT_4497 0.54 +0.8 64,874 - EX28DRAFT_4497 0.54 +0.7 64,874 - EX28DRAFT_4497 0.54 -0.0 64,882 + EX28DRAFT_4497 0.55 -0.1 64,882 + EX28DRAFT_4497 0.55 +0.1 64,882 + EX28DRAFT_4497 0.55 +0.7 64,903 + EX28DRAFT_4497 0.56 +0.6 64,904 - EX28DRAFT_4497 0.56 -0.2
Or see this region's nucleotide sequence