Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4280 and EX28DRAFT_4281 overlap by 17 nucleotides EX28DRAFT_4281 and EX28DRAFT_4282 overlap by 4 nucleotides EX28DRAFT_4282 and EX28DRAFT_4283 are separated by 1 nucleotides EX28DRAFT_4283 and EX28DRAFT_4284 overlap by 4 nucleotides
EX28DRAFT_4280: EX28DRAFT_4280 - Protein of unknown function (DUF2756), at 84,781 to 85,158
_4280
EX28DRAFT_4281: EX28DRAFT_4281 - Glycerophosphoryl diester phosphodiesterase, at 85,142 to 85,888
_4281
EX28DRAFT_4282: EX28DRAFT_4282 - ABC-type sugar transport systems, ATPase components, at 85,885 to 86,955
_4282
EX28DRAFT_4283: EX28DRAFT_4283 - ABC-type sugar transport system, permease component, at 86,957 to 87,802
_4283
EX28DRAFT_4284: EX28DRAFT_4284 - ABC-type sugar transport systems, permease components, at 87,799 to 88,716
_4284
Position (kb)
85
86
87 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 84.942 kb on + strand, within EX28DRAFT_4280 at 85.137 kb on + strand at 85.143 kb on + strand at 85.143 kb on + strand at 85.144 kb on - strand at 85.156 kb on + strand at 85.252 kb on - strand, within EX28DRAFT_4281 at 85.262 kb on - strand, within EX28DRAFT_4281 at 85.720 kb on + strand, within EX28DRAFT_4281 at 85.840 kb on + strand at 85.841 kb on - strand at 86.288 kb on + strand, within EX28DRAFT_4282 at 86.387 kb on + strand, within EX28DRAFT_4282 at 86.387 kb on + strand, within EX28DRAFT_4282 at 86.388 kb on - strand, within EX28DRAFT_4282 at 86.703 kb on - strand, within EX28DRAFT_4282 at 87.174 kb on + strand, within EX28DRAFT_4283 at 87.175 kb on - strand, within EX28DRAFT_4283 at 87.178 kb on + strand, within EX28DRAFT_4283 at 87.178 kb on + strand, within EX28DRAFT_4283 at 87.178 kb on + strand, within EX28DRAFT_4283 at 87.360 kb on - strand, within EX28DRAFT_4283 at 87.418 kb on + strand, within EX28DRAFT_4283 at 87.541 kb on + strand, within EX28DRAFT_4283 at 87.542 kb on - strand, within EX28DRAFT_4283 at 87.720 kb on + strand at 87.721 kb on - strand at 87.763 kb on + strand at 87.763 kb on + strand at 87.764 kb on - strand at 87.816 kb on + strand at 87.828 kb on - strand at 87.934 kb on - strand, within EX28DRAFT_4284 at 87.935 kb on + strand, within EX28DRAFT_4284 at 87.936 kb on - strand, within EX28DRAFT_4284 at 87.936 kb on - strand, within EX28DRAFT_4284 at 87.940 kb on - strand, within EX28DRAFT_4284
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 84,942 + EX28DRAFT_4280 0.43 -0.3 85,137 + -1.4 85,143 + +0.4 85,143 + +0.1 85,144 - +0.3 85,156 + -0.5 85,252 - EX28DRAFT_4281 0.15 +0.3 85,262 - EX28DRAFT_4281 0.16 -0.2 85,720 + EX28DRAFT_4281 0.77 +0.2 85,840 + +0.2 85,841 - -0.1 86,288 + EX28DRAFT_4282 0.38 -0.2 86,387 + EX28DRAFT_4282 0.47 +0.7 86,387 + EX28DRAFT_4282 0.47 -0.4 86,388 - EX28DRAFT_4282 0.47 -0.6 86,703 - EX28DRAFT_4282 0.76 -0.4 87,174 + EX28DRAFT_4283 0.26 +0.1 87,175 - EX28DRAFT_4283 0.26 +1.4 87,178 + EX28DRAFT_4283 0.26 -3.5 87,178 + EX28DRAFT_4283 0.26 -1.0 87,178 + EX28DRAFT_4283 0.26 +0.3 87,360 - EX28DRAFT_4283 0.48 -0.4 87,418 + EX28DRAFT_4283 0.54 +0.1 87,541 + EX28DRAFT_4283 0.69 -1.5 87,542 - EX28DRAFT_4283 0.69 +0.9 87,720 + -0.1 87,721 - -1.7 87,763 + +0.3 87,763 + +0.3 87,764 - -0.8 87,816 + -0.8 87,828 - -1.4 87,934 - EX28DRAFT_4284 0.15 -0.8 87,935 + EX28DRAFT_4284 0.15 -0.3 87,936 - EX28DRAFT_4284 0.15 -0.3 87,936 - EX28DRAFT_4284 0.15 -1.0 87,940 - EX28DRAFT_4284 0.15 +1.1
Or see this region's nucleotide sequence