Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4164 and EX28DRAFT_4165 are separated by 278 nucleotides EX28DRAFT_4165 and EX28DRAFT_4166 are separated by 106 nucleotides EX28DRAFT_4166 and EX28DRAFT_4167 overlap by 11 nucleotides EX28DRAFT_4167 and EX28DRAFT_4168 are separated by 101 nucleotides
EX28DRAFT_4164: EX28DRAFT_4164 - Major Facilitator Superfamily, at 146,150 to 147,334
_4164
EX28DRAFT_4165: EX28DRAFT_4165 - Peptidyl-prolyl cis-trans isomerase (rotamase) - cyclophilin family, at 147,613 to 148,185
_4165
EX28DRAFT_4166: EX28DRAFT_4166 - Protein of unknown function (DUF2559), at 148,292 to 148,459
_4166
EX28DRAFT_4167: EX28DRAFT_4167 - Protein involved in cell division, at 148,449 to 149,042
_4167
EX28DRAFT_4168: EX28DRAFT_4168 - SIR2-like domain, at 149,144 to 152,260
_4168
Position (kb) 148
149 Strain fitness (log2 ratio) -2
-1
0
1
2 at 147.328 kb on + strand at 147.329 kb on - strand at 147.586 kb on + strand at 147.586 kb on + strand at 147.587 kb on - strand at 147.589 kb on - strand at 147.805 kb on + strand, within EX28DRAFT_4165 at 147.805 kb on + strand, within EX28DRAFT_4165 at 147.844 kb on - strand, within EX28DRAFT_4165 at 147.904 kb on - strand, within EX28DRAFT_4165 at 147.904 kb on - strand, within EX28DRAFT_4165 at 147.975 kb on + strand, within EX28DRAFT_4165 at 148.222 kb on + strand at 148.223 kb on - strand at 148.223 kb on - strand at 148.254 kb on + strand at 148.254 kb on + strand at 148.254 kb on + strand at 148.254 kb on + strand at 148.255 kb on - strand at 148.256 kb on + strand at 148.256 kb on + strand at 148.256 kb on + strand at 148.256 kb on + strand at 148.256 kb on + strand at 148.256 kb on + strand at 148.256 kb on + strand at 148.256 kb on + strand at 148.257 kb on - strand at 148.257 kb on - strand at 148.343 kb on - strand, within EX28DRAFT_4166 at 148.343 kb on - strand, within EX28DRAFT_4166 at 148.346 kb on - strand, within EX28DRAFT_4166 at 148.408 kb on - strand, within EX28DRAFT_4166 at 148.416 kb on + strand, within EX28DRAFT_4166 at 148.416 kb on + strand, within EX28DRAFT_4166 at 148.417 kb on - strand, within EX28DRAFT_4166 at 148.417 kb on - strand, within EX28DRAFT_4166 at 148.417 kb on - strand, within EX28DRAFT_4166 at 148.448 kb on + strand at 148.449 kb on - strand at 148.483 kb on - strand at 148.489 kb on - strand at 148.594 kb on + strand, within EX28DRAFT_4167 at 148.595 kb on - strand, within EX28DRAFT_4167 at 148.595 kb on - strand, within EX28DRAFT_4167 at 148.692 kb on - strand, within EX28DRAFT_4167 at 148.695 kb on + strand, within EX28DRAFT_4167 at 148.696 kb on - strand, within EX28DRAFT_4167 at 148.706 kb on + strand, within EX28DRAFT_4167 at 148.728 kb on + strand, within EX28DRAFT_4167 at 148.729 kb on - strand, within EX28DRAFT_4167 at 148.729 kb on - strand, within EX28DRAFT_4167 at 148.730 kb on + strand, within EX28DRAFT_4167 at 148.792 kb on + strand, within EX28DRAFT_4167 at 148.796 kb on + strand, within EX28DRAFT_4167 at 148.949 kb on + strand, within EX28DRAFT_4167 at 148.949 kb on + strand, within EX28DRAFT_4167 at 148.949 kb on + strand, within EX28DRAFT_4167 at 148.949 kb on + strand, within EX28DRAFT_4167 at 148.949 kb on + strand, within EX28DRAFT_4167 at 148.950 kb on - strand, within EX28DRAFT_4167 at 148.950 kb on - strand, within EX28DRAFT_4167 at 148.950 kb on - strand, within EX28DRAFT_4167 at 148.950 kb on - strand, within EX28DRAFT_4167 at 148.950 kb on - strand, within EX28DRAFT_4167 at 148.950 kb on - strand, within EX28DRAFT_4167 at 149.012 kb on - strand at 149.012 kb on - strand at 149.040 kb on + strand at 149.040 kb on + strand at 149.040 kb on + strand at 149.041 kb on - strand at 149.047 kb on + strand at 149.048 kb on - strand at 149.048 kb on - strand at 149.048 kb on - strand at 149.158 kb on + strand at 149.159 kb on - strand at 149.200 kb on - strand at 149.200 kb on - strand at 149.306 kb on - strand at 149.316 kb on - strand at 149.316 kb on - strand at 149.319 kb on + strand at 149.319 kb on + strand at 149.320 kb on - strand at 149.320 kb on - strand at 149.320 kb on - strand at 149.337 kb on - strand at 149.343 kb on + strand at 149.343 kb on + strand at 149.343 kb on + strand at 149.344 kb on - strand at 149.344 kb on - strand at 149.390 kb on + strand at 149.390 kb on + strand at 149.449 kb on + strand at 149.449 kb on + strand at 149.449 kb on + strand at 149.450 kb on - strand at 149.452 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 147,328 + -0.2 147,329 - +0.6 147,586 + +0.3 147,586 + -0.4 147,587 - +0.0 147,589 - +0.4 147,805 + EX28DRAFT_4165 0.34 -0.1 147,805 + EX28DRAFT_4165 0.34 -1.0 147,844 - EX28DRAFT_4165 0.40 -0.3 147,904 - EX28DRAFT_4165 0.51 +0.6 147,904 - EX28DRAFT_4165 0.51 -0.6 147,975 + EX28DRAFT_4165 0.63 +0.3 148,222 + +1.7 148,223 - -0.3 148,223 - +0.4 148,254 + +0.9 148,254 + -0.0 148,254 + -0.6 148,254 + -0.1 148,255 - -0.1 148,256 + +0.1 148,256 + -0.2 148,256 + +0.1 148,256 + +0.6 148,256 + -0.2 148,256 + +1.2 148,256 + -0.2 148,256 + -0.1 148,257 - -0.2 148,257 - +0.9 148,343 - EX28DRAFT_4166 0.30 +0.0 148,343 - EX28DRAFT_4166 0.30 -0.2 148,346 - EX28DRAFT_4166 0.32 -1.6 148,408 - EX28DRAFT_4166 0.69 +0.4 148,416 + EX28DRAFT_4166 0.74 +0.9 148,416 + EX28DRAFT_4166 0.74 +1.6 148,417 - EX28DRAFT_4166 0.74 +0.6 148,417 - EX28DRAFT_4166 0.74 -0.1 148,417 - EX28DRAFT_4166 0.74 -0.6 148,448 + -0.1 148,449 - +0.1 148,483 - +0.6 148,489 - -1.5 148,594 + EX28DRAFT_4167 0.24 -0.8 148,595 - EX28DRAFT_4167 0.25 +0.1 148,595 - EX28DRAFT_4167 0.25 +1.3 148,692 - EX28DRAFT_4167 0.41 -0.3 148,695 + EX28DRAFT_4167 0.41 -0.7 148,696 - EX28DRAFT_4167 0.42 -0.5 148,706 + EX28DRAFT_4167 0.43 -0.5 148,728 + EX28DRAFT_4167 0.47 -0.0 148,729 - EX28DRAFT_4167 0.47 +0.5 148,729 - EX28DRAFT_4167 0.47 +0.1 148,730 + EX28DRAFT_4167 0.47 +0.1 148,792 + EX28DRAFT_4167 0.58 +0.9 148,796 + EX28DRAFT_4167 0.58 +0.0 148,949 + EX28DRAFT_4167 0.84 +1.0 148,949 + EX28DRAFT_4167 0.84 -1.2 148,949 + EX28DRAFT_4167 0.84 +1.0 148,949 + EX28DRAFT_4167 0.84 +0.4 148,949 + EX28DRAFT_4167 0.84 +0.1 148,950 - EX28DRAFT_4167 0.84 -0.3 148,950 - EX28DRAFT_4167 0.84 +2.2 148,950 - EX28DRAFT_4167 0.84 +0.9 148,950 - EX28DRAFT_4167 0.84 +0.4 148,950 - EX28DRAFT_4167 0.84 -0.1 148,950 - EX28DRAFT_4167 0.84 -0.0 149,012 - -1.7 149,012 - -0.4 149,040 + +0.2 149,040 + -0.2 149,040 + +1.7 149,041 - -0.4 149,047 + -0.1 149,048 - +0.8 149,048 - +1.0 149,048 - +0.3 149,158 + -0.6 149,159 - -0.4 149,200 - +0.9 149,200 - +0.4 149,306 - -1.7 149,316 - -0.8 149,316 - +0.0 149,319 + +0.9 149,319 + +0.7 149,320 - -0.6 149,320 - -2.7 149,320 - +1.0 149,337 - +0.6 149,343 + +0.4 149,343 + -0.1 149,343 + +1.9 149,344 - +0.0 149,344 - +0.5 149,390 + -0.7 149,390 + -0.2 149,449 + +0.4 149,449 + -0.2 149,449 + -0.4 149,450 - -0.4 149,452 + +2.1
Or see this region's nucleotide sequence