Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3966 and EX28DRAFT_3967 are separated by 82 nucleotides EX28DRAFT_3967 and EX28DRAFT_3968 overlap by 11 nucleotides EX28DRAFT_3968 and EX28DRAFT_3969 are separated by 92 nucleotides
EX28DRAFT_3966: EX28DRAFT_3966 - D-serine ammonia-lyase (EC 4.3.1.18), at 174,553 to 175,839
_3966
EX28DRAFT_3967: EX28DRAFT_3967 - Domain of unknown function (DUF202), at 175,922 to 176,269
_3967
EX28DRAFT_3968: EX28DRAFT_3968 - Predicted membrane protein, at 176,259 to 176,606
_3968
EX28DRAFT_3969: EX28DRAFT_3969 - Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases, at 176,699 to 178,021
_3969
Position (kb) 175
176
177 Strain fitness (log2 ratio) -2
-1
0
1
2 at 174.922 kb on + strand, within EX28DRAFT_3966 at 174.922 kb on + strand, within EX28DRAFT_3966 at 174.922 kb on + strand, within EX28DRAFT_3966 at 174.923 kb on - strand, within EX28DRAFT_3966 at 174.923 kb on - strand, within EX28DRAFT_3966 at 174.923 kb on - strand, within EX28DRAFT_3966 at 174.989 kb on - strand, within EX28DRAFT_3966 at 175.014 kb on + strand, within EX28DRAFT_3966 at 175.119 kb on + strand, within EX28DRAFT_3966 at 175.119 kb on + strand, within EX28DRAFT_3966 at 175.119 kb on + strand, within EX28DRAFT_3966 at 175.119 kb on + strand, within EX28DRAFT_3966 at 175.119 kb on + strand, within EX28DRAFT_3966 at 175.120 kb on - strand, within EX28DRAFT_3966 at 175.120 kb on - strand, within EX28DRAFT_3966 at 175.370 kb on - strand, within EX28DRAFT_3966 at 175.376 kb on - strand, within EX28DRAFT_3966 at 175.502 kb on - strand, within EX28DRAFT_3966 at 175.633 kb on + strand, within EX28DRAFT_3966 at 175.658 kb on - strand, within EX28DRAFT_3966 at 175.658 kb on - strand, within EX28DRAFT_3966 at 175.820 kb on - strand at 175.820 kb on - strand at 175.838 kb on - strand at 175.838 kb on - strand at 175.841 kb on + strand at 175.842 kb on - strand at 175.873 kb on + strand at 175.891 kb on + strand at 175.891 kb on + strand at 175.891 kb on + strand at 176.003 kb on - strand, within EX28DRAFT_3967 at 176.078 kb on + strand, within EX28DRAFT_3967 at 176.270 kb on - strand at 176.339 kb on + strand, within EX28DRAFT_3968 at 176.341 kb on + strand, within EX28DRAFT_3968 at 176.648 kb on + strand at 176.648 kb on + strand at 176.649 kb on - strand at 176.718 kb on + strand at 176.719 kb on - strand at 176.959 kb on + strand, within EX28DRAFT_3969 at 177.060 kb on + strand, within EX28DRAFT_3969 at 177.060 kb on + strand, within EX28DRAFT_3969 at 177.104 kb on - strand, within EX28DRAFT_3969 at 177.223 kb on - strand, within EX28DRAFT_3969 at 177.225 kb on + strand, within EX28DRAFT_3969 at 177.225 kb on + strand, within EX28DRAFT_3969 at 177.225 kb on + strand, within EX28DRAFT_3969 at 177.225 kb on + strand, within EX28DRAFT_3969 at 177.226 kb on - strand, within EX28DRAFT_3969 at 177.226 kb on - strand, within EX28DRAFT_3969 at 177.226 kb on - strand, within EX28DRAFT_3969 at 177.234 kb on + strand, within EX28DRAFT_3969 at 177.234 kb on + strand, within EX28DRAFT_3969 at 177.234 kb on + strand, within EX28DRAFT_3969
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 174,922 + EX28DRAFT_3966 0.29 +0.3 174,922 + EX28DRAFT_3966 0.29 -0.3 174,922 + EX28DRAFT_3966 0.29 +0.9 174,923 - EX28DRAFT_3966 0.29 -0.5 174,923 - EX28DRAFT_3966 0.29 -1.0 174,923 - EX28DRAFT_3966 0.29 -0.1 174,989 - EX28DRAFT_3966 0.34 -2.6 175,014 + EX28DRAFT_3966 0.36 +1.3 175,119 + EX28DRAFT_3966 0.44 -2.1 175,119 + EX28DRAFT_3966 0.44 -0.6 175,119 + EX28DRAFT_3966 0.44 +1.0 175,119 + EX28DRAFT_3966 0.44 +1.4 175,119 + EX28DRAFT_3966 0.44 +0.3 175,120 - EX28DRAFT_3966 0.44 -1.1 175,120 - EX28DRAFT_3966 0.44 +2.6 175,370 - EX28DRAFT_3966 0.63 -0.1 175,376 - EX28DRAFT_3966 0.64 +0.0 175,502 - EX28DRAFT_3966 0.74 +0.2 175,633 + EX28DRAFT_3966 0.84 -1.0 175,658 - EX28DRAFT_3966 0.86 +0.0 175,658 - EX28DRAFT_3966 0.86 -0.3 175,820 - +0.8 175,820 - +0.7 175,838 - +0.7 175,838 - +0.1 175,841 + +0.5 175,842 - +1.0 175,873 + -2.1 175,891 + -0.3 175,891 + +0.1 175,891 + -0.6 176,003 - EX28DRAFT_3967 0.23 +0.8 176,078 + EX28DRAFT_3967 0.45 +0.0 176,270 - +1.1 176,339 + EX28DRAFT_3968 0.23 +0.2 176,341 + EX28DRAFT_3968 0.24 +0.4 176,648 + -1.3 176,648 + -0.2 176,649 - +0.1 176,718 + -0.1 176,719 - +0.7 176,959 + EX28DRAFT_3969 0.20 -0.4 177,060 + EX28DRAFT_3969 0.27 +1.2 177,060 + EX28DRAFT_3969 0.27 +0.3 177,104 - EX28DRAFT_3969 0.31 -0.9 177,223 - EX28DRAFT_3969 0.40 -0.7 177,225 + EX28DRAFT_3969 0.40 -0.2 177,225 + EX28DRAFT_3969 0.40 +1.1 177,225 + EX28DRAFT_3969 0.40 -0.4 177,225 + EX28DRAFT_3969 0.40 +0.6 177,226 - EX28DRAFT_3969 0.40 +0.2 177,226 - EX28DRAFT_3969 0.40 -0.1 177,226 - EX28DRAFT_3969 0.40 +0.5 177,234 + EX28DRAFT_3969 0.40 +0.5 177,234 + EX28DRAFT_3969 0.40 -1.0 177,234 + EX28DRAFT_3969 0.40 -1.0
Or see this region's nucleotide sequence