Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3652 and EX28DRAFT_3653 are separated by 74 nucleotides EX28DRAFT_3653 and EX28DRAFT_3654 are separated by 47 nucleotides EX28DRAFT_3654 and EX28DRAFT_3655 are separated by 10 nucleotides
EX28DRAFT_3652: EX28DRAFT_3652 - DNA polymerase elongation subunit (family B), at 316,848 to 319,205
_3652
EX28DRAFT_3653: EX28DRAFT_3653 - L-ribulose-5-phosphate 4-epimerase, at 319,280 to 319,975
_3653
EX28DRAFT_3654: EX28DRAFT_3654 - L-arabinose isomerase, at 320,023 to 321,525
_3654
EX28DRAFT_3655: EX28DRAFT_3655 - ribulokinase, at 321,536 to 323,245
_3655
Position (kb) 320
321
322 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 319.179 kb on + strand at 319.211 kb on + strand at 319.255 kb on - strand at 319.260 kb on - strand at 319.269 kb on - strand at 319.270 kb on + strand at 319.270 kb on + strand at 319.271 kb on - strand at 319.272 kb on + strand at 319.272 kb on + strand at 319.273 kb on - strand at 319.275 kb on - strand at 319.294 kb on - strand at 319.320 kb on + strand at 319.392 kb on + strand, within EX28DRAFT_3653 at 319.393 kb on - strand, within EX28DRAFT_3653 at 319.393 kb on - strand, within EX28DRAFT_3653 at 319.393 kb on - strand, within EX28DRAFT_3653 at 319.393 kb on - strand, within EX28DRAFT_3653 at 319.444 kb on + strand, within EX28DRAFT_3653 at 319.608 kb on + strand, within EX28DRAFT_3653 at 319.609 kb on - strand, within EX28DRAFT_3653 at 319.769 kb on - strand, within EX28DRAFT_3653 at 319.919 kb on - strand at 319.970 kb on + strand at 319.971 kb on - strand at 319.971 kb on - strand at 320.020 kb on + strand at 320.021 kb on - strand at 320.024 kb on + strand at 320.025 kb on - strand at 320.417 kb on + strand, within EX28DRAFT_3654 at 320.417 kb on + strand, within EX28DRAFT_3654 at 320.418 kb on - strand, within EX28DRAFT_3654 at 320.418 kb on - strand, within EX28DRAFT_3654 at 320.516 kb on + strand, within EX28DRAFT_3654 at 320.517 kb on - strand, within EX28DRAFT_3654 at 320.517 kb on - strand, within EX28DRAFT_3654 at 320.522 kb on + strand, within EX28DRAFT_3654 at 321.018 kb on + strand, within EX28DRAFT_3654 at 321.048 kb on + strand, within EX28DRAFT_3654 at 321.282 kb on + strand, within EX28DRAFT_3654 at 321.282 kb on + strand, within EX28DRAFT_3654 at 321.283 kb on - strand, within EX28DRAFT_3654 at 321.312 kb on + strand, within EX28DRAFT_3654 at 321.321 kb on - strand, within EX28DRAFT_3654 at 321.321 kb on - strand, within EX28DRAFT_3654 at 321.321 kb on - strand, within EX28DRAFT_3654 at 321.506 kb on + strand at 321.506 kb on + strand at 321.506 kb on + strand at 321.506 kb on + strand at 321.506 kb on + strand at 321.506 kb on + strand at 321.506 kb on + strand at 321.506 kb on + strand at 321.506 kb on + strand at 321.507 kb on - strand at 321.507 kb on - strand at 321.507 kb on - strand at 321.507 kb on - strand at 321.507 kb on - strand at 321.639 kb on + strand at 322.221 kb on + strand, within EX28DRAFT_3655 at 322.222 kb on - strand, within EX28DRAFT_3655 at 322.222 kb on - strand, within EX28DRAFT_3655 at 322.226 kb on - strand, within EX28DRAFT_3655
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 319,179 + -0.1 319,211 + +0.3 319,255 - -0.1 319,260 - -0.2 319,269 - -0.7 319,270 + +0.7 319,270 + +0.0 319,271 - +1.3 319,272 + +0.6 319,272 + -1.5 319,273 - +0.2 319,275 - -1.4 319,294 - -0.1 319,320 + +0.1 319,392 + EX28DRAFT_3653 0.16 +0.5 319,393 - EX28DRAFT_3653 0.16 +0.4 319,393 - EX28DRAFT_3653 0.16 +2.4 319,393 - EX28DRAFT_3653 0.16 +0.1 319,393 - EX28DRAFT_3653 0.16 +1.0 319,444 + EX28DRAFT_3653 0.24 +0.1 319,608 + EX28DRAFT_3653 0.47 -0.5 319,609 - EX28DRAFT_3653 0.47 -0.3 319,769 - EX28DRAFT_3653 0.70 -0.2 319,919 - +0.3 319,970 + +0.4 319,971 - +0.2 319,971 - +1.5 320,020 + -2.0 320,021 - +0.3 320,024 + +0.1 320,025 - +0.0 320,417 + EX28DRAFT_3654 0.26 -1.3 320,417 + EX28DRAFT_3654 0.26 +0.7 320,418 - EX28DRAFT_3654 0.26 -1.0 320,418 - EX28DRAFT_3654 0.26 -0.2 320,516 + EX28DRAFT_3654 0.33 -0.4 320,517 - EX28DRAFT_3654 0.33 -0.1 320,517 - EX28DRAFT_3654 0.33 +0.5 320,522 + EX28DRAFT_3654 0.33 -1.1 321,018 + EX28DRAFT_3654 0.66 -1.1 321,048 + EX28DRAFT_3654 0.68 -2.4 321,282 + EX28DRAFT_3654 0.84 +1.6 321,282 + EX28DRAFT_3654 0.84 +0.5 321,283 - EX28DRAFT_3654 0.84 +1.5 321,312 + EX28DRAFT_3654 0.86 -1.0 321,321 - EX28DRAFT_3654 0.86 -1.1 321,321 - EX28DRAFT_3654 0.86 +0.0 321,321 - EX28DRAFT_3654 0.86 -2.9 321,506 + -0.9 321,506 + +0.3 321,506 + -0.1 321,506 + +0.6 321,506 + +0.5 321,506 + -0.1 321,506 + -0.0 321,506 + -1.3 321,506 + -0.0 321,507 - -0.3 321,507 - +0.9 321,507 - -0.1 321,507 - +0.3 321,507 - +0.5 321,639 + -0.3 322,221 + EX28DRAFT_3655 0.40 -1.3 322,222 - EX28DRAFT_3655 0.40 +0.2 322,222 - EX28DRAFT_3655 0.40 -1.4 322,226 - EX28DRAFT_3655 0.40 -0.5
Or see this region's nucleotide sequence