Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3516 and EX28DRAFT_3517 are separated by 152 nucleotides EX28DRAFT_3517 and EX28DRAFT_3518 overlap by 8 nucleotides EX28DRAFT_3518 and EX28DRAFT_3519 overlap by 26 nucleotides
EX28DRAFT_3516: EX28DRAFT_3516 - NAD-dependent aldehyde dehydrogenases, at 181,306 to 182,721
_3516
EX28DRAFT_3517: EX28DRAFT_3517 - Uncharacterized enzyme involved in biosynthesis of extracellular polysaccharides, at 182,874 to 183,197
_3517
EX28DRAFT_3518: EX28DRAFT_3518 - ACT domain-containing protein, at 183,190 to 183,585
_3518
EX28DRAFT_3519: EX28DRAFT_3519 - Predicted transcriptional regulators, at 183,560 to 184,255
_3519
Position (kb)
182
183
184 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 182.118 kb on + strand, within EX28DRAFT_3516 at 182.300 kb on - strand, within EX28DRAFT_3516 at 182.300 kb on - strand, within EX28DRAFT_3516 at 182.515 kb on + strand, within EX28DRAFT_3516 at 182.644 kb on - strand at 182.644 kb on - strand at 182.799 kb on + strand at 182.799 kb on + strand at 182.799 kb on + strand at 182.800 kb on - strand at 183.129 kb on + strand, within EX28DRAFT_3517 at 183.130 kb on - strand, within EX28DRAFT_3517 at 183.193 kb on + strand at 183.193 kb on + strand at 183.193 kb on + strand at 183.194 kb on - strand at 183.364 kb on - strand, within EX28DRAFT_3518 at 183.522 kb on + strand, within EX28DRAFT_3518 at 183.522 kb on + strand, within EX28DRAFT_3518 at 183.523 kb on - strand, within EX28DRAFT_3518 at 183.523 kb on - strand, within EX28DRAFT_3518 at 183.559 kb on + strand at 183.559 kb on + strand at 183.559 kb on + strand at 183.560 kb on - strand at 183.560 kb on - strand at 183.577 kb on - strand at 183.577 kb on - strand at 183.586 kb on + strand at 183.806 kb on + strand, within EX28DRAFT_3519 at 183.926 kb on + strand, within EX28DRAFT_3519 at 183.962 kb on + strand, within EX28DRAFT_3519
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 182,118 + EX28DRAFT_3516 0.57 -0.1 182,300 - EX28DRAFT_3516 0.70 +0.8 182,300 - EX28DRAFT_3516 0.70 -0.1 182,515 + EX28DRAFT_3516 0.85 -0.7 182,644 - -0.4 182,644 - -2.2 182,799 + -2.0 182,799 + -0.6 182,799 + +1.0 182,800 - +0.1 183,129 + EX28DRAFT_3517 0.79 -0.5 183,130 - EX28DRAFT_3517 0.79 -1.1 183,193 + +0.6 183,193 + +0.5 183,193 + +0.3 183,194 - +0.3 183,364 - EX28DRAFT_3518 0.44 +0.2 183,522 + EX28DRAFT_3518 0.84 +0.3 183,522 + EX28DRAFT_3518 0.84 +0.3 183,523 - EX28DRAFT_3518 0.84 -1.1 183,523 - EX28DRAFT_3518 0.84 +0.0 183,559 + -1.0 183,559 + -0.8 183,559 + -0.2 183,560 - +1.2 183,560 - +0.2 183,577 - +0.6 183,577 - +0.5 183,586 + +0.5 183,806 + EX28DRAFT_3519 0.35 +2.5 183,926 + EX28DRAFT_3519 0.53 +3.3 183,962 + EX28DRAFT_3519 0.58 +1.6
Or see this region's nucleotide sequence