Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3333 and EX28DRAFT_3334 overlap by 25 nucleotides EX28DRAFT_3334 and EX28DRAFT_3335 are separated by 113 nucleotides
EX28DRAFT_3333: EX28DRAFT_3333 - Thiol:disulfide interchange protein, at 2,937 to 4,652
_3333
EX28DRAFT_3334: EX28DRAFT_3334 - Uncharacterized protein involved in tolerance to divalent cations, at 4,628 to 4,951
_3334
EX28DRAFT_3335: EX28DRAFT_3335 - anaerobic c4-dicarboxylate membrane transporter family protein, at 5,065 to 6,366
_3335
Position (kb) 4
5 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 3.644 kb on - strand, within EX28DRAFT_3333 at 3.644 kb on - strand, within EX28DRAFT_3333 at 3.644 kb on - strand, within EX28DRAFT_3333 at 3.644 kb on - strand, within EX28DRAFT_3333 at 3.685 kb on - strand, within EX28DRAFT_3333 at 3.688 kb on + strand, within EX28DRAFT_3333 at 3.688 kb on + strand, within EX28DRAFT_3333 at 3.689 kb on - strand, within EX28DRAFT_3333 at 3.689 kb on - strand, within EX28DRAFT_3333 at 3.689 kb on - strand, within EX28DRAFT_3333 at 3.689 kb on - strand, within EX28DRAFT_3333 at 3.904 kb on + strand, within EX28DRAFT_3333 at 3.904 kb on + strand, within EX28DRAFT_3333 at 3.905 kb on - strand, within EX28DRAFT_3333 at 3.905 kb on - strand, within EX28DRAFT_3333 at 3.905 kb on - strand, within EX28DRAFT_3333 at 3.934 kb on - strand, within EX28DRAFT_3333 at 3.970 kb on + strand, within EX28DRAFT_3333 at 3.971 kb on - strand, within EX28DRAFT_3333 at 3.994 kb on + strand, within EX28DRAFT_3333 at 3.994 kb on + strand, within EX28DRAFT_3333 at 3.998 kb on + strand, within EX28DRAFT_3333 at 3.998 kb on + strand, within EX28DRAFT_3333 at 3.998 kb on + strand, within EX28DRAFT_3333 at 3.999 kb on - strand, within EX28DRAFT_3333 at 4.073 kb on - strand, within EX28DRAFT_3333 at 4.215 kb on - strand, within EX28DRAFT_3333 at 4.354 kb on - strand, within EX28DRAFT_3333 at 4.354 kb on - strand, within EX28DRAFT_3333 at 4.477 kb on + strand, within EX28DRAFT_3333 at 4.477 kb on + strand, within EX28DRAFT_3333 at 4.477 kb on + strand, within EX28DRAFT_3333 at 4.478 kb on - strand, within EX28DRAFT_3333 at 4.478 kb on - strand, within EX28DRAFT_3333 at 4.478 kb on - strand, within EX28DRAFT_3333 at 4.478 kb on - strand, within EX28DRAFT_3333 at 4.478 kb on - strand, within EX28DRAFT_3333 at 4.478 kb on - strand, within EX28DRAFT_3333 at 4.478 kb on - strand, within EX28DRAFT_3333 at 4.481 kb on - strand at 4.502 kb on + strand at 4.660 kb on - strand at 4.818 kb on + strand, within EX28DRAFT_3334 at 4.818 kb on + strand, within EX28DRAFT_3334 at 4.983 kb on + strand at 4.983 kb on + strand at 4.983 kb on + strand at 4.983 kb on + strand at 4.983 kb on + strand at 4.984 kb on - strand at 4.984 kb on - strand at 4.984 kb on - strand at 4.984 kb on - strand at 4.986 kb on - strand at 5.039 kb on + strand at 5.040 kb on - strand at 5.043 kb on + strand at 5.066 kb on + strand at 5.156 kb on + strand at 5.157 kb on - strand at 5.157 kb on - strand at 5.381 kb on + strand, within EX28DRAFT_3335 at 5.442 kb on + strand, within EX28DRAFT_3335 at 5.442 kb on + strand, within EX28DRAFT_3335 at 5.649 kb on - strand, within EX28DRAFT_3335 at 5.873 kb on + strand, within EX28DRAFT_3335 at 5.874 kb on - strand, within EX28DRAFT_3335
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 3,644 - EX28DRAFT_3333 0.41 +1.1 3,644 - EX28DRAFT_3333 0.41 -0.5 3,644 - EX28DRAFT_3333 0.41 -1.5 3,644 - EX28DRAFT_3333 0.41 +1.1 3,685 - EX28DRAFT_3333 0.44 -0.5 3,688 + EX28DRAFT_3333 0.44 -0.0 3,688 + EX28DRAFT_3333 0.44 +0.8 3,689 - EX28DRAFT_3333 0.44 -0.1 3,689 - EX28DRAFT_3333 0.44 +2.8 3,689 - EX28DRAFT_3333 0.44 -1.8 3,689 - EX28DRAFT_3333 0.44 -0.5 3,904 + EX28DRAFT_3333 0.56 +0.4 3,904 + EX28DRAFT_3333 0.56 +0.5 3,905 - EX28DRAFT_3333 0.56 -0.2 3,905 - EX28DRAFT_3333 0.56 +0.4 3,905 - EX28DRAFT_3333 0.56 +1.1 3,934 - EX28DRAFT_3333 0.58 +1.4 3,970 + EX28DRAFT_3333 0.60 -0.5 3,971 - EX28DRAFT_3333 0.60 +0.5 3,994 + EX28DRAFT_3333 0.62 +0.7 3,994 + EX28DRAFT_3333 0.62 +1.0 3,998 + EX28DRAFT_3333 0.62 -0.0 3,998 + EX28DRAFT_3333 0.62 +0.1 3,998 + EX28DRAFT_3333 0.62 +0.1 3,999 - EX28DRAFT_3333 0.62 +0.0 4,073 - EX28DRAFT_3333 0.66 -0.1 4,215 - EX28DRAFT_3333 0.74 -0.1 4,354 - EX28DRAFT_3333 0.83 +0.7 4,354 - EX28DRAFT_3333 0.83 +0.4 4,477 + EX28DRAFT_3333 0.90 -1.5 4,477 + EX28DRAFT_3333 0.90 -2.5 4,477 + EX28DRAFT_3333 0.90 -0.4 4,478 - EX28DRAFT_3333 0.90 -0.2 4,478 - EX28DRAFT_3333 0.90 -0.5 4,478 - EX28DRAFT_3333 0.90 -0.5 4,478 - EX28DRAFT_3333 0.90 +0.6 4,478 - EX28DRAFT_3333 0.90 -0.4 4,478 - EX28DRAFT_3333 0.90 -1.1 4,478 - EX28DRAFT_3333 0.90 -0.4 4,481 - -0.6 4,502 + +0.2 4,660 - -1.0 4,818 + EX28DRAFT_3334 0.59 +1.3 4,818 + EX28DRAFT_3334 0.59 -0.2 4,983 + -0.0 4,983 + +0.9 4,983 + +2.5 4,983 + -1.2 4,983 + +0.5 4,984 - -0.5 4,984 - -1.8 4,984 - -1.3 4,984 - -0.3 4,986 - -3.0 5,039 + +0.5 5,040 - +0.6 5,043 + -1.6 5,066 + +0.4 5,156 + +0.7 5,157 - -2.1 5,157 - -0.8 5,381 + EX28DRAFT_3335 0.24 +0.2 5,442 + EX28DRAFT_3335 0.29 +0.2 5,442 + EX28DRAFT_3335 0.29 -0.1 5,649 - EX28DRAFT_3335 0.45 +0.3 5,873 + EX28DRAFT_3335 0.62 -0.5 5,874 - EX28DRAFT_3335 0.62 +1.5
Or see this region's nucleotide sequence