Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3313 and EX28DRAFT_3314 are separated by 23 nucleotides EX28DRAFT_3314 and EX28DRAFT_3315 are separated by 177 nucleotides EX28DRAFT_3315 and EX28DRAFT_3316 are separated by 139 nucleotides EX28DRAFT_3316 and EX28DRAFT_3317 overlap by 85 nucleotides
EX28DRAFT_3313: EX28DRAFT_3313 - putative TIM-barrel protein, nifR3 family, at 613,350 to 614,315
_3313
EX28DRAFT_3314: EX28DRAFT_3314 - Factor for inversion stimulation Fis, transcriptional activator, at 614,339 to 614,635
_3314
EX28DRAFT_3315: EX28DRAFT_3315 - Protein of unknown function (DUF2556), at 614,813 to 614,977
_3315
EX28DRAFT_3316: EX28DRAFT_3316 - diguanylate cyclase (GGDEF) domain, at 615,117 to 617,198
_3316
EX28DRAFT_3317: EX28DRAFT_3317 - transcriptional regulator, TetR family, at 617,114 to 617,833
_3317
Position (kb) 615
616
617
618 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 614.257 kb on + strand at 614.272 kb on + strand at 614.499 kb on + strand, within EX28DRAFT_3314 at 614.637 kb on - strand at 614.647 kb on + strand at 614.726 kb on + strand at 614.727 kb on - strand at 614.768 kb on + strand at 614.768 kb on + strand at 614.769 kb on - strand at 614.771 kb on - strand at 614.771 kb on - strand at 614.817 kb on + strand at 614.821 kb on + strand at 614.822 kb on - strand at 614.822 kb on - strand at 614.822 kb on - strand at 614.822 kb on - strand at 614.826 kb on - strand at 614.895 kb on + strand, within EX28DRAFT_3315 at 614.926 kb on + strand, within EX28DRAFT_3315 at 615.060 kb on - strand at 615.106 kb on + strand at 615.132 kb on + strand at 615.132 kb on + strand at 615.132 kb on + strand at 615.183 kb on + strand at 615.184 kb on - strand at 615.260 kb on + strand at 615.336 kb on + strand, within EX28DRAFT_3316 at 615.337 kb on - strand, within EX28DRAFT_3316 at 615.358 kb on + strand, within EX28DRAFT_3316 at 615.376 kb on + strand, within EX28DRAFT_3316 at 615.421 kb on + strand, within EX28DRAFT_3316 at 615.422 kb on - strand, within EX28DRAFT_3316 at 615.476 kb on + strand, within EX28DRAFT_3316 at 615.484 kb on - strand, within EX28DRAFT_3316 at 615.484 kb on - strand, within EX28DRAFT_3316 at 615.684 kb on + strand, within EX28DRAFT_3316 at 616.025 kb on + strand, within EX28DRAFT_3316 at 616.130 kb on + strand, within EX28DRAFT_3316 at 616.253 kb on - strand, within EX28DRAFT_3316 at 616.320 kb on - strand, within EX28DRAFT_3316 at 616.338 kb on + strand, within EX28DRAFT_3316 at 616.352 kb on + strand, within EX28DRAFT_3316 at 616.471 kb on - strand, within EX28DRAFT_3316 at 616.542 kb on - strand, within EX28DRAFT_3316 at 616.831 kb on + strand, within EX28DRAFT_3316 at 616.911 kb on + strand, within EX28DRAFT_3316 at 616.994 kb on + strand at 616.995 kb on - strand at 617.126 kb on + strand at 617.149 kb on + strand at 617.149 kb on + strand at 617.150 kb on - strand at 617.150 kb on - strand at 617.150 kb on - strand at 617.150 kb on - strand at 617.150 kb on - strand at 617.180 kb on - strand at 617.196 kb on + strand at 617.196 kb on + strand at 617.197 kb on - strand at 617.243 kb on + strand, within EX28DRAFT_3317 at 617.243 kb on + strand, within EX28DRAFT_3317 at 617.282 kb on - strand, within EX28DRAFT_3317 at 617.315 kb on + strand, within EX28DRAFT_3317 at 617.316 kb on - strand, within EX28DRAFT_3317 at 617.415 kb on - strand, within EX28DRAFT_3317 at 617.467 kb on + strand, within EX28DRAFT_3317 at 617.488 kb on + strand, within EX28DRAFT_3317 at 617.537 kb on - strand, within EX28DRAFT_3317 at 617.538 kb on + strand, within EX28DRAFT_3317 at 617.538 kb on + strand, within EX28DRAFT_3317 at 617.539 kb on - strand, within EX28DRAFT_3317 at 617.711 kb on - strand, within EX28DRAFT_3317 at 617.890 kb on - strand at 618.038 kb on + strand at 618.156 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 614,257 + +0.1 614,272 + +1.8 614,499 + EX28DRAFT_3314 0.54 -0.1 614,637 - -0.5 614,647 + +0.2 614,726 + -2.3 614,727 - +0.4 614,768 + -0.6 614,768 + +0.0 614,769 - +0.5 614,771 - +0.0 614,771 - +1.7 614,817 + +0.2 614,821 + -2.2 614,822 - +0.1 614,822 - +0.5 614,822 - -0.3 614,822 - +0.4 614,826 - -1.1 614,895 + EX28DRAFT_3315 0.50 -2.3 614,926 + EX28DRAFT_3315 0.68 -1.2 615,060 - -0.5 615,106 + +1.3 615,132 + -0.2 615,132 + +0.0 615,132 + +0.4 615,183 + +0.5 615,184 - -1.1 615,260 + -0.4 615,336 + EX28DRAFT_3316 0.11 -0.1 615,337 - EX28DRAFT_3316 0.11 -0.6 615,358 + EX28DRAFT_3316 0.12 +0.2 615,376 + EX28DRAFT_3316 0.12 +0.5 615,421 + EX28DRAFT_3316 0.15 +1.1 615,422 - EX28DRAFT_3316 0.15 -0.8 615,476 + EX28DRAFT_3316 0.17 +0.8 615,484 - EX28DRAFT_3316 0.18 +0.4 615,484 - EX28DRAFT_3316 0.18 -3.7 615,684 + EX28DRAFT_3316 0.27 -0.1 616,025 + EX28DRAFT_3316 0.44 +0.4 616,130 + EX28DRAFT_3316 0.49 +0.1 616,253 - EX28DRAFT_3316 0.55 +0.8 616,320 - EX28DRAFT_3316 0.58 +0.4 616,338 + EX28DRAFT_3316 0.59 +1.7 616,352 + EX28DRAFT_3316 0.59 -0.5 616,471 - EX28DRAFT_3316 0.65 +0.4 616,542 - EX28DRAFT_3316 0.68 +1.1 616,831 + EX28DRAFT_3316 0.82 +0.5 616,911 + EX28DRAFT_3316 0.86 +0.0 616,994 + -3.3 616,995 - +0.5 617,126 + -0.1 617,149 + +0.6 617,149 + -0.1 617,150 - -0.3 617,150 - -0.5 617,150 - -0.1 617,150 - +0.7 617,150 - +0.4 617,180 - +1.1 617,196 + -1.4 617,196 + -0.3 617,197 - -0.2 617,243 + EX28DRAFT_3317 0.18 +1.7 617,243 + EX28DRAFT_3317 0.18 -0.8 617,282 - EX28DRAFT_3317 0.23 -0.1 617,315 + EX28DRAFT_3317 0.28 -0.4 617,316 - EX28DRAFT_3317 0.28 -0.3 617,415 - EX28DRAFT_3317 0.42 -1.1 617,467 + EX28DRAFT_3317 0.49 -0.1 617,488 + EX28DRAFT_3317 0.52 +1.8 617,537 - EX28DRAFT_3317 0.59 -0.3 617,538 + EX28DRAFT_3317 0.59 -1.3 617,538 + EX28DRAFT_3317 0.59 +0.3 617,539 - EX28DRAFT_3317 0.59 +1.5 617,711 - EX28DRAFT_3317 0.83 -0.4 617,890 - +0.7 618,038 + -0.2 618,156 + +0.8
Or see this region's nucleotide sequence